REL PDBe PDBe PDBe 2013-11-04 2013-12-11 2014-03-26 2014-03-26 Cryo-electron tomography average of an C1-IgG complex Diebolder CA Beurskens FJ de Jong RN Koning RI Strumane K Lindorfer MA Voorhorst M Ugurlar D Rosati S Heck AJR van de Winkel JGJ Wilson IA Koster AJ Taylor RP Ollmann-Saphire E Burton DR Schuurman J Gros P Parren PWHI C1-IgG complex, C1-immune complex, C1, C1qr2S2, IgG, classical complement activation Diebolder CA Beurskens FJ de Jong RN Koning RI Strumane K Lindorfer MA Voorhorst M Ugurlar D Rosati S Heck AJR van de Winkel JGJ Wilson IA Koster AJ Taylor RP Ollmann Saphire E Burton DR Schuurman J Gros P Parren PWHI Complement is activated by IgG hexamers assembled at the cell surface. SCIENCE 343 1260 1263 2014 24626930 doi:10.1126/science.1248943 DNP-haptenated liposomes, polyclonal goat anti-DNP Antibody, human C1 (originating from C4 depleted serum) DNP-haptenated liposomes, polyclonal goat anti-DNP Antibody, human C1 (originating from C4 depleted serum) one C1 complex binds to one hexamer of membrane bound IgG 2 1.65 polyclonal IgG Capra hircus Goat blood 0.15 6 Hexamer false C1 Homo sapiens Human Blood 0.766 1 monomer false subtomogramAveraging particle ETHANE-PROPANE MIXTURE 95 LEICA EM GP Blot 3 seconds before plunging FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.7 5.0 7.5 50000.0 FEI TITAN KRIOS AUTOGRID HOLDER Gatan Quantum SE/963 2012-06-06 GATAN ULTRASCAN 4000 (4k x 4k) 51 100 16. -60 60
particle selected using IMOD stalkInit. C6 for IgG hexamer (lower platform) C2 for C1r2s2 hetero tetramer (upper platform)
C2 OTHER 66.0 OTHER IMOD, PEET, Spider
Final map is an average of 107 particles, representing the first class after pca classification and k-means clustering of 200 particles. The 200 particles originate from ~50 single and dual axis tomograms
107
Phase flipping in IMOD after defocus esimation in TOMOCTF
 emd_2506.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 174 174 174 0 0 0 174 174 174 748.2 748.2 748.2 90.0 90.0 90.0 X Y Z -0.00031829 1.00010979 0.3666459 0.09346347 4.3 4.3 4.3 0.5 AUTHOR C1-IgG
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