REL PDBe PDBe PDBe 2014-11-19 2014-12-10 2014-12-10 2015-03-11 Semi-automated selection of cryo-EM particles in RELION-1.3 Scheres SHW single-particle analysis, automated particle picking, beta-galactosidase Scheres SH Semi-automated selection of cryo-EM particles in RELION-1.3. J.STRUCT.BIOL. 189 114 122 2015 25486611 doi:10.1016/j.jsb.2014.11.010 beta-galactosidase beta-galactosidase hometetramer 1 0.45 beta-galactosidase Escherichia coli K-12 0.465 4 tetramer false P00722 GO:0004565 singleParticle particle 1
glow-discharged Quantifoil grids
 ETHANE 90 HOMEMADE PLUNGER manual blotting FEI POLARA 300 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2 1.359 4.962 81000.0 79096.0 OTHER 2012-06-12 FEI FALCON II (4k x 4k) 84 24
Every image is the average of 24 raw (unaligned) movie frames. The complete set of electron micrographs used to obtain the density map presented here is available through the Electron Microscopy Pilot Image ARchive (EMPIAR).
 0
The particles were selected using the new semi-automatic particle selection procedures in RELION-1.3. This reconstruction was done to test these procedures.
RELION
 D2 4.2 OTHER RELION, CTFFIND3 42755
RELION
 emd_2824.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 180 180 180 0 0 0 180 180 180 318.6 318.6 318.6 90.0 90.0 90.0 X Y Z -0.41184917 0.74745452 0.00064884 0.03866324 1.77 1.77 1.77 0.2 AUTHOR Reconstruction with semi-automatically selected particles.
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 emd_2824_fsc.xml