REL PDBe PDBe PDBe 2014-12-10 2014-12-24 2014-12-24 2015-03-11 Cryo-EM structure of TMV reconstructed from data ranging from 6.1-10.2 e-/A^2 accumulated dose Fromm SA Bharat TAM Jakobi AJ Hagen WJH Sachse C tobacco mosaic virus, direct electron detectors, single particle helical reconstruction, radiation damage Fromm SA Bharat TA Jakobi AJ Hagen WJ Sachse C Seeing tobacco mosaic virus through direct electron detectors. J.STRUCT.BIOL. 189 87 97 2015 25528571 doi:10.1016/j.jsb.2014.12.002 Tobacco Mosaic Virus Tobacco Mosaic Virus
The sample was purified from infected leaves.
helical 1 39.5 Tobacco mosaic virus Tobacco mosaic virus vulgare Nicotiana tabacum PLANTAE(HIGHER PLANTS) 39.5 CP 180 VIRION STRAIN false false TMV helical filament 11.0 7.0
50 mM NaPO4
Quantifoil 2/2 Cu 200 mesh grids
 ETHANE 90 95 FEI VITROBOT MARK III Blot for 8 seconds with an offset of -2 mm ~30-45 seconds after sample application FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 1.0 4.5 75000.0 131827.0 FEI TITAN KRIOS AUTOGRID HOLDER CTFTILT
Nanoprobe mode, dose rate ~41 e-/px/s on the camera level, fully automated acquisition using FEI EPU software
2013-10-16 FEI FALCON II (4k x 4k) 109 30.7
each micrograph was recorded as a movie of 7 frames excluding the roll-in frame corresponding to 2 e-/A^2 accumulated dose which was discarded
Image processing was done using SPRING.
1.408 22.03 C1 OTHER 3.5 OTHER SPRING
final map was calculated only from frame 2 of each micrograph corresponding to an accumulated dose range of 6.1-10.2 e-/A^2
CTFTILT (specific for each segment)
SPIDER
 emd_2836.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 210 210 190 -105 -105 -95 210 210 190 223.02 223.02 201.78001 90.0 90.0 90.0 X Y Z -3.52556205 5.769382 0.01445695 0.72779012 1.062 1.062 1.062 1.2 AUTHOR Cryo-EM structure of TMV reconstructed from data ranging from 6.1-10.2 e-/A^2 accumulated dose
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emd_2836_fsc.xml