2025-09-17 REL PDBj INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBj PDBj 2024-03-11 2025-09-17 2025-09-17 2025-09-17 Japan Agency for Medical Research and Development (AMED) JP17am0101093 Japan Japan Agency for Medical Research and Development (AMED) JP20ae0101047 Japan Japan Agency for Medical Research and Development (AMED) JP21fk0108463 Japan Japan Agency for Medical Research and Development (AMED) JP22ama121037 Japan Japan Agency for Medical Research and Development (AMED) JP223fa627005 Japan Japan Agency for Medical Research and Development (AMED) JP23ama121001 Japan Japan Science and Technology JPMJCR20H8 Japan Ministry of Education, Culture, Sports, Science and Technology (Japan) JP20H05873 Japan STA map of C3 symmetry spike protein from active SARS-CoV-2 vririons Dokainish HM Maenaka K virions, spike protein, VIRAL PROTEIN Dokainish HM Fukuhara H Maenaka K Unprecedented spike flexibility revealed by BSL3 Cryo-ET of active SARS-CoV-2 virions To Be Published 0353 EMDB EMD-39422 associated EM volume Severe acute respiratory syndrome coronavirus 2 Severe acute respiratory syndrome coronavirus 2 0 1 Severe acute respiratory syndrome coronavirus 2 Wuhan VeroE6/TMPRSS2 VIRION STRAIN true false SARS-CoV-2 Spike protein Severe acute respiratory syndrome coronavirus 2 Wuhan LEVO MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFS NVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIV NNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLE GKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQT LLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETK CTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISN CVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIAD YNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPC NGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVN FNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITP GTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSY ECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTI SVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQE VFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDC LGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAM QMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALN TLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRA SANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPA ICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDP LQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDL QELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDD SEPVLKGVKLHYT subtomogramAveraging particle 7.4
Opti-MEM
 ETHANE 90 291 LEICA EM GP TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.2 0.2 64000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN VOLTA PHASE PLATE TFS Selectris FEI FALCON IV (4k x 4k) 4096 4096 0.73 1.5 1 1 C3 FOURIER SPACE 14.3 FSC 0.143 CUT-OFF RELION 4.0 237 42 237 manual RELION 4.0 RELION 4.0 PHASE FLIPPING AND AMPLITUDE CORRECTION MAXIMUM LIKELIHOOD RELION 4.0 emd_39422.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 144 144 144 0 0 0 144 144 144 271.296 271.296 271.296 90.0 90.0 90.0 X Y Z -3.5068357 10.916169999999999 0.03317533 1.195578 1.884 1.884 1.884 4.75 AUTHOR emd_39422_msk_1.map emd_39422_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 144 144 144 0 0 0 144 144 144 271.296 271.296 271.296 90.0 90.0 90.0 X Y Z -18.068366999999999 28.559989999999999 0.027784381 2.2481956 1.884 1.884 1.884 AUTHOR emd_39422_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 144 144 144 0 0 0 144 144 144 271.296 271.296 271.296 90.0 90.0 90.0 X Y Z -33.757232999999999 34.755671999999997 0.03697271 3.1758301 1.884 1.884 1.884 AUTHOR