2026-04-15 REL PDBj INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY STRUCTURE_SUMMARY citation citation_author em_admin struct _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.pdbx_database_id_DOI _citation.title _citation.year _em_admin.last_update _em_admin.title _struct.title EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.pdbx_database_id_DOI _citation.title _citation.year _em_admin.last_update _em_admin.title PDBj PDBj 2024-04-30 2025-11-05 2025-11-05 2026-04-15 Japan Agency for Medical Research and Development (AMED) Japan Japan Society for the Promotion of Science (JSPS) Japan Cryo-EM structure of eSaCas9_NNG-guide RNA-target DNA complex in an intermediate state Omura SN Nakagawa R Yamashita K Nishimasu H Nureki O CRISPR-CAS9, GENOME ENGINEERING, HYDROLASE-RNA-DNA COMPLEX, DNA BINDING PROTEIN Omura SN Nakagawa R Kajimoto S Okazaki S Ishiguro S Mori H Onishi K Kashiwakura Y Hiramoto T Hirano H Yamashita K Jividen K Tsai SQ Yachie N Ohmori T Nishimasu H Nureki O Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation. Nat Commun UK 2026 doi:10.1038/s41467-026-71626-2 2041-1723 8zcz FULLOVERLAP EMDB EMD-39942 associated EM volume
Cryo-EM structure of eSaCas9_NNG-guide RNA-target DNA complex in an interrogation state
 eSaCas9-NNG-sgRNA-target DNA ternary complex in an intermediaitie state eSaCas9-NNG-sgRNA-target DNA ternary complex in an intermediaitie state 0 1 2 3 4 Staphylococcus aureus CRISPR-associated endonuclease Cas9 Staphylococcus aureus 0.124305898 1 Escherichia coli LEVO GSHMKRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEGRRSKRGARRLKRRRRHRIQRVKKLLFDYNLL TDHSELSGINPYEARVKGLSQKLSEEEFSAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLER LKKDGEVRGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHC TYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTS TGKPEFTNLKVYHDIKDITARKEIIENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSL KAINLILDELWHTNDAQIAIFNALKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIKVINAIIKKYGLPNDIIIE LAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDH IIPRSVSFDNSFNNKVLVKQEENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFSV QKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERNKGYKHHAEDALIIANADFIFKE WKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIFITPHQIKHIKDFKDYKYSHRVDKKPNRKLINDTLYSTRKDDKG NTRIVNNLNGLYDKDNDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKDNGPVI KKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVKVNNLDVIKKENYYEVNSKCYEEAKKLKKIS NQAEFIASFYRNDLIKINGELYRVIGVASDLLNAIEVNMIDITYREYLENMNDKRPPRIFKTISSKTQSIKKYSTDILGN LYEVKSKKHPQIIKKG J7RUA5 3.1.-.- sgRNA Staphylococcus aureus 0.031892889 1 GGGAAAUUAGGUGCGCUUGGCGUUUUAGUACUCUGGAAACAGAAUCUACUAAAACAAGGCAAAAUGCCGUGUUUAUCUCG UCAACUUGUUGGCGAGAUA Target DNA strand Staphylococcus aureus 0.013314968999999998 1 (DA)(DT)(DT)(DT)(DG)(DA)(DA)(DT)(DA)(DG)(DG)(DC)(DA)(DA)(DG)(SC)(SC)(AS)(AS)(GS) (SC)(GS)(DC)(DA)(DC)(DC)(DT)(DA)(DA)(DT)(DT)(DT)(DC)(DC)(DC)(DG)(DT)(DA)(DT)(DT) (DT)(DA)(DG) DNA Non-target DNA strand Staphylococcus aureus 0.013386005999999999 1 (DC)(DT)(DA)(DA)(DA)(DT)(DA)(DC)(DG)(DG)(DG)(DA)(DA)(DA)(DT)(DT)(DA)(DG)(DG)(DT) (DG)(SC)(GS)(SC)(PST)(PST)(GS)(GS)(DC)(DT)(DT)(DG)(DC)(DC)(DT)(DA)(DT)(DT)(DC) (DA)(DA)(DA)(DT) DNA singleParticle particle 7.6 ETHANE TFS KRIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 0.8 1.6 GATAN K3 (6k x 4k) 50.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 2.9 FSC 0.143 CUT-OFF 156154 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_39942.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 154 154 154 58 58 58 154 154 154 170.4318 170.4318 170.4318 90.0 90.0 90.0 X Y Z -0.46317148 0.7884243 0.0025240323 0.022741605 1.1067 1.1067 1.1067 0.06 AUTHOR Locally filtered a sharpened map using a local resolution map. emd_39942_msk_1.map emd_39942_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 154 154 154 58 58 58 154 154 154 170.4318 170.4318 170.4318 90.0 90.0 90.0 X Y Z -0.15358546 0.4590651 0.00088094873 0.020739684 1.1067 1.1067 1.1067 AUTHOR emd_39942_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 154 154 154 58 58 58 154 154 154 170.4318 170.4318 170.4318 90.0 90.0 90.0 X Y Z -0.1588998 0.46198878 0.0008945049 0.020749547 1.1067 1.1067 1.1067 AUTHOR