2026-03-04 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2023-12-09 2026-03-04 2026-03-04 2026-03-04 National Science Foundation (NSF, United States) 1818255 United States Ecoli DnaB helicase and Phage Lambda loader P with ADP-Mg in a 6:5 stoichiometry ratio Shatarupa A Brown D Olinares PDB Chase J Isiorho E Chait BT Jeruzalmi D Hexameric DnaB helicase, Phage Lambda P helicase loader, bacterial DNA replication initiation, auto inhibition, DNA BINDING PROTEIN Shatarupa A Brown D Olinares PDB Chase J Isiorho E Chait BT Jeruzalmi D Distinct quaternary states, intermediates, and autoinhibition during loading of the DnaB-replicative helicase by the phage lambda P helicase loader. Nucleic Acids Res. UK 53 2025 41312769 doi:10.1093/nar/gkaf1139 1362-4962 0389 NARHAD Brown D Shatarupa A Olinares PDB Chase J Isiorho E Chait BT Jeruzalmi D An Autoinhibited Conformation of the DnaB-Replicative Helicase- phage LP Helicase Loader Complex Biorxiv US 2025 doi:10.1101/2022.12.30.522210 2692-8205 0353 8v9t FULLOVERLAP EMDB EMD-43082 associated EM volume
Escherichia coli helicase DnaB and Phage Lambda loader P in an auto-inhibited conformation, at 2.84 resolution
 Ecoli DnaB helicase and Phage Lambda loader P with ADP-Mg in a 6:5 stoichiometry ratio. Ecoli DnaB helicase and Phage Lambda loader P with ADP-Mg in a 6:5 stoichiometry ratio. 0 1 2 0.44694 Replicative DNA helicase Escherichia coli 0.052450945 6 Escherichia coli BL21(DE3) LEVO MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGS PIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTF AMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTP TEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNS DLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE P0ACB0 3.6.4.12 Helicase loader Escherichia phage Lambda 0.026551326 5 Escherichia coli BL21(DE3) LEVO MENIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVFSQLLATFPASLANRDQNEVNEIRRQWVLAFRENGIT TMEQVNAGMRVARRQNRPFLPSPGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL YQNMRANALTDAELRRKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAKIAEIKAKFGLKGASV P03689 ADENOSINE-5'-DIPHOSPHATE 0.000427201 5 ADP MAGNESIUM ION 2.4305e-05 5 MG singleParticle particle 4.98 7.5 20.0 C8H17N2NaO4S HEPES-Sodium salt 450.0 NaCl Sodium Chloride 2.0 C4H10O2S2 Dithiothreitol (DTT) 0.5 MgCl2 Magnesium Chloride 0.2 C10H16N5O13P3 Adenosine triphosphate (ATP) 0.25 C3H8O3 Glycerol
20 mM Na-HEPES pH 7.5, 450mM NaCl, 2mM DTT, 0.5mM MgCl2, 0.2mM ATP, 0.25% Glycerol
 Quantifoil R0.6/1 GOLD 300 GOLD HOLEY PLASMA CLEANING OTHER ETHANE 100 277 FEI VITROBOT MARK IV
Protein BP (1.5 uM) and DNA (1.875 uM) was mixed in a 1.25 molar excess. 3uL of the sample was added to a plasma-cleaned grid at 4 degrees celsius, 100 percent humidity, blot force 4, blot time 4s, wait time 30s, total blots 1, and plunge-frozen into liquid nitrogen-cooled ethane..
TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 1.0 2.5 81000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Bioquantum 20 GATAN K3 (6k x 4k) 1 25595 2.0 51.01 1 12437695 PHASE FLIPPING AND AMPLITUDE CORRECTION 1B79 C1 FOURIER SPACE 2.84 FSC 0.143 CUT-OFF cryoSPARC 1137545 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_43082.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 346.56 346.56 346.56 90.0 90.0 90.0 X Y Z -0.0018494652 2.2886662 0.00223351 0.034839943 1.083 1.083 1.083 0.135 AUTHOR The attached map has been sharpened using the DeepEMhancer software. 1B79 A 16-127 PDB experimental model A 128-471 AlphaFold in silico model A 2-118 AlphaFold in silico model 8V9S A 119-192 PDB experimental model A 193-233 AlphaFold in silico model OTHER REAL emd_43082_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 346.56 346.56 346.56 90.0 90.0 90.0 X Y Z -0.37596276 1.049091 0.0010788254 0.028983463 1.083 1.083 1.083 AUTHOR emd_43082_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 346.56 346.56 346.56 90.0 90.0 90.0 X Y Z -0.38535908 1.0986984 0.0010772168 0.02903293 1.083 1.083 1.083 AUTHOR