2025-12-03 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY EXPERIMENTAL_SUMMARY em_admin em_author_list _em_admin.last_update _em_author_list.author STRUCTURE_SUMMARY audit_author em_admin em_author_list _audit_author.name _em_admin.last_update _em_author_list.author DATABASE_REFERENCES citation citation_author em_admin _em_admin.last_update RCSB RCSB 2024-07-26 2025-08-13 2025-08-13 2025-12-03 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) NIGMS R01-128087 United States BmrCD in the outward-facing conformation bound to Hoechsts Tang Q Mchaourab HS ABC transporter, Exporter, membrane protein, transport Tang Q Sinclair M Bisignano P Zhang Y Tajkhorshid E Mchaourab HS Drug-bound outward-facing conformation of a heterodimeric ABC exporter suggests a putative mechanism of drug translocation. Nat Commun UK 16 10403 10403 2025 41285748 doi:10.1038/s41467-025-65318-6 2041-1723 Tang Q Sinclair M Bisignano P Zhang Y Tajkhorshid E Mchaourab HS Lipid-mediated mechanism of drug extrusion by a heterodimeric ABC exporter. Biorxiv US 2025 40060395 doi:10.1101/2025.02.26.640354 2692-8205 EMD-40974 other EM volume EMD-29297 other EM volume EMD-46002 consensus EM volume EMD-46003 focused EM volume 9cus FULLOVERLAP EMDB EMD-40974 other EM volume EMDB EMD-29297 other EM volume EMDB EMD-45940 associated EM volume
BmrCD in outward-facing conformation with Hoechsts
 EMDB EMD-46002 consensus EM volume EMDB EMD-46003 focused EM volume PDB 8T1P unspecified PDB 8FMV unspecified Heterodimeric BmrCD with ligands ATP and lipids Heterodimeric BmrCD with ligands ATP and lipids 0 1 2 Bacillus subtilis subsp. subtilis str. 168 Probable multidrug resistance ABC transporter ATP-binding/permease protein YheI Bacillus subtilis subsp. subtilis str. 168 0.067589922 1 Escherichia coli 'BL21-Gold(DE3)pLysS AG' LEVO MGSSHHHHHHSSGLVPRGSHMLEFSVLKKLGWFFKAYWLRYTIAIVLLLAVNVIEMFPPKLLGNAIDDMKAGAFTAEGLL FYIGIFFVLTAAVYIMSYFWMHQLFGGANLMEKILRTKLMGHLLTMSPPFYEKNRTGDLMARGTNDLQAVSLTTGFGILT LVDSTMFMMTIFLTMGFLISWKLTFAAIIPLPVMAIAISLYGSKIHERFTEAQNAFGALNDRVLESVSGVRVIRAYVQET NDVRRFNEMTADVYQKNMKVAFIDSLFEPTVKLLVGASYLIGLGYGAFLVFRNELTLGELVSFNVYLGMMIWPMFAIGEL INVMQRGNASLDRVNETLSYETDVTDPKQPADLKEPGDIVFSHVSFTYPSSTSDNLQDISFTVRKGQTVGIAGKTGSGKT TIIKQLLRQYPPGEGSITFSGVPIQQIPLDRLRGWIGYVPQDHLLFSRTVKENILYGKQDATDKEVQQAIAEAHFEKDLH MLPSGLETMVGEKGVALSGGQKQRISIARALMANPEILILDDSLSAVDAKTEAAIIKNIRENRKGKTTFILTHRLSAVEH ADLILVMDGGVIAERGTHQELLANNGWYREQYERQQLFTAEEGGAGA O07550 7.6.2.- Probable multidrug resistance ABC transporter ATP-binding/permease protein YheH Bacillus subtilis subsp. subtilis str. 168 0.077467078 1 Escherichia coli 'BL21-Gold(DE3)pLysS AG' LEVO MKIGKTLWRYALLYRKLLITAVLLLTVAVGAELTGPFIGKKMIDDHILGIEKTWYEAAEKDKNAVQFHGVSYVREDRLQE PVSKAKEAHIYQVGMAFYFVDQAVSFDGNRTVSDGKLTITNGDKSRAYAAEKLTKQELFQFYQPEIKGMVLLICLYGGLL VFSVFFQYGQHYLLQMSANRIIQKMRQDVFSHIQKMPIRYFDNLPAGKVVARITNDTEAIRDLYVTVLSTFVTSGIYMFG IFTALFLLDVKLAFVCLAIVPIIWLWSVIYRRYASYYNQKIRSINSDINAKMNESIQGMTIIQAFRHQKETMREFEELNE SHFYFQNRMLNLNSLMSHNLVNVIRNLAFVCLIWHFGGASLNAAGIVSIGVLYAFVDYLNRLFQPITGIVNQFSKLELAR VSAGRVFELLEEKNTEEAGEPAKERALGRVEFRDVSFAYQEGEEVLKHISFTAQKGETVALVGHTGSGKSSILNLLFRFY DAQKGDVLIDGKSIYNMSRQELRSHMGIVLQDPYLFSGTIGSNVSLDDERMTEEEIKNALRQVGAEPLLKKLPKGINEPV IEKGSTLSSGERQLISFARALAFDPAILILDEATAHIDTETEAVIQKALDVVKQGRTTFVIAHRLSTIRNADQILVLDKG EIVERGNHEELMALEGQYYQMYELQKGQKHSIALEHHHHHH O07549 7.6.2.- MAGNESIUM ION 2.4305e-05 2 MG ADENOSINE-5'-TRIPHOSPHATE 0.000507181 1 ATP 2'-(4-ETHOXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5'-BI-BENZIMIDAZOLE 0.000452551 2 HT1 (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate 0.0007600759999999999 20 POV ADP ORTHOVANADATE 0.000544156 1 AOV singleParticle particle 3 8.0 ETHANE 100 277 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.4 2.2 NITROGEN GATAN K3 (6k x 4k) 15.0 1 NONE 8FHK 3.1 FSC 0.143 CUT-OFF 27802 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_45940.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 310.56 310.56 310.56 90.0 90.0 90.0 X Y Z -0.23529638 0.5577568 0.003139707 0.012311411 0.647 0.647 0.647 0.05 AUTHOR BmrCD in outward-facing conformation with Hoechsts emd_45940_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 310.56 310.56 310.56 90.0 90.0 90.0 X Y Z -0.33862033 0.49054942 -0.00028632677 0.0075804093 0.647 0.647 0.647 AUTHOR Additional Map A emd_45940_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 310.56 310.56 310.56 90.0 90.0 90.0 X Y Z -0.30560574 0.5577568 -0.00027922855 0.015513088 0.647 0.647 0.647 AUTHOR Additional Map B