2026-01-21 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2024-08-28 2026-01-21 2026-01-21 2026-01-21 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R01GM071940 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R01AI101057 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) RO1AI152408 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R01AI177658 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R01AI113157 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35GM145249 United States National Natural Science Foundation of China (NSFC) 32570759 China Trypanosoma brucei mitochondrial RNA-editing catalytic complex 1, U-deletion (RECC1), right wing focused refinement map Liu YT Jih J Zhou ZH Aphasizhev R RNA editing, tRNA, OB-fold, mitochondria, RNA BINDING PROTEIN Liu YT Vacas AF Jih J Zhao X Yu C Lee JKJ Suematsu T Solayman M Wang H Wang X Huang L Zhang L Aphasizheva I Zhou ZH Aphasizhev R Structural basis of the mitochondrial RNA editing cascade in trypanosomes To Be Published 0353 EMDB EMD-46787 associated EM volume RECC1 RECC1 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
From T. brucei mitochondrial T2 isolate
Trypanosoma brucei Lister 427 Mitochondria singleParticle particle 7.6 60.0 KCl Potassium chloride 25.0 Tris-HCl Tris hydrochloride 10.0 MgCl2 Magnesium chloride 5.0 OG n-Octylglucoside 1.0 CaCl2 Calcium chloride Quantifoil R1.2/1.3 GOLD 300 GRAPHENE CONTINUOUS PLASMA CLEANING ETHANE 100 277 FEI VITROBOT MARK IV
From T. brucei mitochondrial T2 isolate
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 1.0 3.0 81000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Bioquantum 20 GATAN K3 BIOQUANTUM (6k x 4k) 23092 2.0 45.0 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 0.6 2.8000000000000003 130000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN TFS Selectris X 10 FEI FALCON IV (4k x 4k) 40988 3.5 45.0 2
Falcon 4i and K3 Bioquantum images were combined for processing.
cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION C1 FOURIER SPACE 4.07 FSC 0.143 CUT-OFF cryoSPARC 93504 MAXIMUM LIKELIHOOD cryoSPARC MAXIMUM LIKELIHOOD cryoSPARC cryoSPARC emd_46787.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 422.40002 422.40002 422.40002 90.0 90.0 90.0 X Y Z -0.71033406 1.2369173 0.0008488558 0.020558294 1.1 1.1 1.1 0.24 AUTHOR RNA-editing catalytic complex 1 (RECC1), right wing focused refinement map AB INITIO MODEL
Consensus map and focused refinement maps were combined to generate a composite map for final model refinement.
REAL emd_46787_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 422.40002 422.40002 422.40002 90.0 90.0 90.0 X Y Z -1.597594 1.5080863 0.00086540915 0.13523331 1.1 1.1 1.1 AUTHOR RNA-editing catalytic complex 1 (RECC1), right wing focused refinement half map A emd_46787_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 422.40002 422.40002 422.40002 90.0 90.0 90.0 X Y Z -1.4903113 1.5006995 0.0008742051 0.13707489 1.1 1.1 1.1 AUTHOR RNA-editing catalytic complex 1 (RECC1), right wing focused refinement half map B