2025-07-30 REL RCSB RCSB RCSB 2024-09-02 2025-07-30 2025-07-30 2025-07-30 National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) 75N93019C00051 United States Han J Rodriguez AJ Ferguson JA Ward AB influenza, hemagglutinin, H1, polyclonal, complex, Fab complex, viral fusion protein, VIRAL PROTEIN Sangesland M Li N Tsybovsky Y Rodgers MD Han J Rodriguez AJ Ferguson JA Henry AR Smith SC Roberts-Torres J Gillespie RA Liu C Merriam JS Stephens T Williams C Maestle E Corcoran M Ravichandran M Creanga A Andrews SF Pierson TC Karlsson Hedestam GB Schramm CA Reed DS Douek DC Zhou T Ward AB Kanekiyo M Biorxiv US 2025 40568173 doi:10.1101/2025.02.21.639368 2692-8205 EMD-46824 other EM volume EMD-46825 other EM volume EMD-46827 other EM volume EMD-46829 other EM volume EMD-46830 other EM volume EMD-46832 other EM volume Polyclonal immune complex of Fab from Cynomolgus Macaque T009 at week 12 binding H1 HA Polyclonal immune complex of Fab from Cynomolgus Macaque T009 at week 12 binding H1 HA 0 Macaca fascicularis 0.28 singleParticle particle 0.015 7.4 NEGATIVE 2% w/v uranyl formate Homemade COPPER 400 CARBON FEI TECNAI SPIRIT FLOOD BEAM DARK FIELD LAB6 200 0.5 2.0 SIDE ENTRY, EUCENTRIC NITROGEN FEI EAGLE (4k x 4k) 25.0 1 NONE 4M4Y 20.0 FSC 0.5 CUT-OFF RELION 3.0 8974 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_46831_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 208 208 208 0 0 0 208 208 208 428.47998 428.47998 428.47998 90.0 90.0 90.0 X Y Z -0.025982106 0.08939674 0.00026664903 0.0038667514 2.06 2.06 2.06 AUTHOR ::::EMDATABANK.org::::EMD-46831:::: Negative stain map of polyclonal Fab binding the H1 HA anchor epitope from Rhesus Macaque T009 at week 12 emd_46831_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 208 208 208 0 0 0 208 208 208 428.47998 428.47998 428.47998 90.0 90.0 90.0 X Y Z -0.023081506 0.08495325 0.00033772373 0.0034825506 2.06 2.06 2.06 AUTHOR ::::EMDATABANK.org::::EMD-46831:::: Negative stain map of polyclonal Fab binding the H1 HA central stem 2 epitope from Rhesus Macaque T009 at week 12 emd_46831_additional_3.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 208 208 208 0 0 0 208 208 208 428.47998 428.47998 428.47998 90.0 90.0 90.0 X Y Z -0.020264173 0.085271955 0.00027696084 0.0034436649 2.06 2.06 2.06 AUTHOR ::::EMDATABANK.org::::EMD-46831:::: Negative stain map of polyclonal Fab binding the H1 HA low stem epitope from Rhesus Macaque T009 at week 12 emd_46831_additional_4.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 208 208 208 0 0 0 208 208 208 428.47998 428.47998 428.47998 90.0 90.0 90.0 X Y Z -0.033554908 0.10386684 0.0002611043 0.0041505885 2.06 2.06 2.06 AUTHOR ::::EMDATABANK.org::::EMD-46831:::: Negative stain map of polyclonal Fab binding the H1 HA central stem 1 epitope from Rhesus Macaque T009 at week 12 emd_46831_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 208 208 208 0 0 0 208 208 208 428.47998 428.47998 428.47998 90.0 90.0 90.0 X Y Z -0.032248627 0.097070545 0.0002636929 0.003997783 2.06 2.06 2.06 AUTHOR ::::EMDATABANK.org::::EMD-46831:::: Negative stain half map A for global refinement of particle stack used to sort polyclonal responses to H1 HA from Cynomolgus Macaque T009 at week 12 emd_46831_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 208 208 208 0 0 0 208 208 208 428.47998 428.47998 428.47998 90.0 90.0 90.0 X Y Z -0.028897846 0.09392203 0.00025872525 0.0039690416 2.06 2.06 2.06 AUTHOR ::::EMDATABANK.org::::EMD-46831:::: Negative stain half map B for global refinement of particle stack used to sort polyclonal responses to H1 HA from Cynomolgus Macaque T009 at week 12