2026-01-21 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2024-10-10 2026-01-21 2026-01-21 2026-01-21 National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK) R01 DK131118-01 United States National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK) R01 DK123463-01 United States National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK) (F32DK137477-01A1 United States National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK) TL1DK132769 United States National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK) U2CDK129917 United States PKD2 ion channel, F634A mutant Esarte Palomero O DeCaen PG Ion channel, TRP channel, polycystin, membrane protein Esarte Palomero O DeCaen PG PKD2 ion channel, F629S variant To Be Published 0353 9dwt FULLOVERLAP EMDB EMD-47264 associated EM volume
PKD2 ion channel, F634A mutant
 PKD2 ion channel F634A mutant protomer PKD2 ion channel F634A mutant protomer 0 1 Homo sapiens 0.3396 Polycystin-2 Homo sapiens 0.056038362999999994 4 Homo sapiens LEVO EKYLKSVLRELVTYLLFLIVLCILTYGMMSSNVYYYTRMMSQLFLDTPVSKTEKTNFKTLSSMEDFWKFTEGSLLDGLYW KMQPSNQTEADNRSFIFYENLLLGVPRIRQLRVRNGSCSIPQDLRDEIKECYDVYSVSSEDRAPFGPRNGTAWIYTSEKD LNGSSHWGIIATYSGAGYYLDLSRTREETAAQVASLKKNVWLDRGTRATFIDFSVYNANINLFCVVRLLVEFPATGGVIP SWQFQPLKLIRYVTTFDFFLAACEIIFCFFIFYYVVEEILEIRIHKLHYFRSFWNCLDVVIVVLSVVAIGINIYRTSNVE VLLQFLEDQNTFPNFEHLAYWQIQFNNIAAVTVFFVWIKLFKFINFNRTMSQLSTTMSRCAKDLFGFAIMFFIIFLAYAQ LAYLVFGTQVDDFSTFQECIATQFRIILGDINFAEIEEANRVLGPIYFTTFVFFMFFILLNMFLAIINDTYSEVKSDLAQ Q13563 CALCIUM ION 4.0078e-05 1 CA singleParticle particle 1.0 7.4 150.0 NaCl Sodium Chloride 25.0 HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) 1.0 CaCl2 Calcium Chloride 1.0 TCEP tris(2-carboxyethyl)phosphine
25 mM HEPES-NaOH, 150 mM NaCl, 1 mM CaCl2, 1 mM TCEP
 Quantifoil R2/1 COPPER 300 CARBON HOLEY ARRAY GLOW DISCHARGE AIR 0.026000000000000002 ETHANE 95 278 LEICA EM GP
Vitrification carried in air. Ethane temperature -183 C.
Stabilized in amphipol A8-35. Monodisperse sample after gel filtration in a Superdex 200 column.
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.6 2.4 130000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Bioquantum 10 GATAN K3 (6k x 4k) 11520 8184 1 14969 60.0
50 frame movie stacks. Super-resolution mode
 1 1441572
Micrographs were blob picked (130-170 angstrom)
 cryoSPARC 4.5 PHASE FLIPPING AND AMPLITUDE CORRECTION Ab-initio reconstruction C4 FOURIER SPACE 3.01 FSC 0.143 CUT-OFF cryoSPARC 4.5
Final reconstruction was generated using C4 symmetry
98122 MAXIMUM LIKELIHOOD cryoSPARC 4.5 MAXIMUM LIKELIHOOD cryoSPARC 4.5 3 cryoSPARC emd_47264.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 259.52002 259.52002 259.52002 90.0 90.0 90.0 X Y Z -0.2961952 0.4370261 0.0003364614 0.015294541 0.6488001 0.6488001 0.6488001 0.071 AUTHOR PKD2 mutant F634A sharpened EM map 180-925 AlphaFold in silico model
The initial Alphafold model was fitted to the map using ChimeraX. Refinement was performed using ISOLDE and PHENIX.
REAL 101.0 emd_47264_msk_1.map emd_47264_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 259.52002 259.52002 259.52002 90.0 90.0 90.0 X Y Z -0.07286199 0.14416093 0.0003364614 0.005837615 0.6488001 0.6488001 0.6488001 AUTHOR PKD2 mutant F634A unsharpened EM map emd_47264_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 259.52002 259.52002 259.52002 90.0 90.0 90.0 X Y Z -0.6307246 0.5381444 0.0003419772 0.04235154 0.6488001 0.6488001 0.6488001 AUTHOR PKD2 mutant F634A EM Half map B emd_47264_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 259.52002 259.52002 259.52002 90.0 90.0 90.0 X Y Z -0.40837428 0.3976595 0.0003433511 0.04239377 0.6488001 0.6488001 0.6488001 AUTHOR PKD2 mutant F634A EM Half map A