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    <admin>
        <current_status>
            <date>2026-01-21</date>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <revision_history>
            <revision version="1.0" date="2026-01-21">
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                        <revision_type>INITIAL_RELEASE</revision_type>
                        <provider>REPOSITORY</provider>
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        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2024-10-10</deposition>
            <header_release>2026-01-21</header_release>
            <map_release>2026-01-21</map_release>
            <update>2026-01-21</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</funding_body>
                <code>GR128399</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</funding_body>
                <code>T32GM144293</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)</funding_body>
                <code>GR137328</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>EcRuvB T102R hexamer assembly</title>
        <authors_list>
            <author>Rish AD</author>
            <author>Fu TM</author>
        </authors_list>
        <keywords>ATPase, pre-assembly, complex, beta-hairpin, MOTOR PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author ORCID="0000-0002-2789-9699" order="1">Rish AD</author>
                    <author ORCID="0000-0002-6281-1752" order="2">Fu TM</author>
                    <title>Pre-assembly regulation of AAA+ DNA motor protein</title>
                    <journal_abbreviation>To Be Published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
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                <pdb_id>9dx5</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
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                <db_name>EMDB</db_name>
                <accession_id>EMD-47281</accession_id>
                <content_type>associated EM volume</content_type>
                <details>EcRuvB T102R hexamer assembly</details>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>Pre-assembled RuvB</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Pre-assembled RuvB</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <details>T102R hexameric assembly</details>
                <natural_source database="NCBI">
                    <organism ncbi="83333">Escherichia coli K-12</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Holliday junction branch migration complex subunit RuvB</name>
                <natural_source database="NCBI">
                    <organism ncbi="83333">Escherichia coli K-12</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.037271773</theoretical>
                </molecular_weight>
                <details>T102R mutant</details>
                <number_of_copies>6</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="469008">Escherichia coli BL21(DE3)</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLRNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP</string>
                    <external_references type="UNIPROTKB">P0A812</external_references>
                </sequence>
                <ec_number>3.6.4.-</ec_number>
            </protein_or_peptide>
            <ligand macromolecule_id="2">
                <name>ADENOSINE-5'-DIPHOSPHATE</name>
                <molecular_weight>
                    <theoretical units="MDa">0.000427201</theoretical>
                </molecular_weight>
                <number_of_copies>1</number_of_copies>
                <formula>ADP</formula>
            </ligand>
        </macromolecule_list>
    </sample>
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        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">1.5</concentration>
                    <buffer>
                        <ph>7.5</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_defocus_min units="µm">0.5</nominal_defocus_min>
                    <nominal_defocus_max units="µm">1.5</nominal_defocus_max>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 (6k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">50.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
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                <image_recording_id>1</image_recording_id>
                <ctf_correction>
                    <type>NONE</type>
                </ctf_correction>
                <startup_model type_of_model="NONE"/>
                <final_reconstruction>
                    <resolution units="Å" res_type="BY AUTHOR">3.74</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <number_images_used>185318</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>NOT APPLICABLE</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>NOT APPLICABLE</type>
                </final_angle_assignment>
            </singleparticle_processing>
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            <c units="Å">273.92</c>
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        <label>::::EMDATABANK.org::::EMD-47281::::</label>
        <annotation_details>DeepEmhanced map</annotation_details>
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        <modelling_list>
            <modelling>
                <refinement_protocol>RIGID BODY FIT</refinement_protocol>
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                <label>::::EMDATABANK.org::::EMD-47281::::</label>
                <annotation_details>half map B</annotation_details>
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                    <b units="Å">273.92</b>
                    <c units="Å">273.92</c>
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                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
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                    <y units="Å">1.07</y>
                    <z units="Å">1.07</z>
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                    <contour primary="true">
                        <source>AUTHOR</source>
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                <label>::::EMDATABANK.org::::EMD-47281::::</label>
                <annotation_details>half map A</annotation_details>
            </half_map>
        </half_map_list>
    </interpretation>
</emd>
