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        <current_status>
            <date>2026-01-21</date>
            <code>REL</code>
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        <key_dates>
            <deposition>2024-10-11</deposition>
            <header_release>2026-01-21</header_release>
            <map_release>2026-01-21</map_release>
            <update>2026-01-21</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Science Foundation (NSF, United States)</funding_body>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>attPmm and attBmm bound serine integrase complex in the pre-rotation state</title>
        <authors_list>
            <author>Shin H</author>
            <author>Rice PA</author>
            <author>Olorunniji FJ</author>
        </authors_list>
        <keywords>Viral protein, Integrase, Recombinase, Complex, Recombination Directionality Factor, Integration, Excision, DNA BINDING PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author order="1">Shin HS</author>
                    <author order="2">Rice PA</author>
                    <title>Structural Basis of Directionality Control in Large Serine Integrases</title>
                    <journal_abbreviation>To Be Published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
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                <details>consensus map of attPmm and attBmm bound integrase complex in the  pre-rotation state</details>
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                <details>focused map of attPmm and attBmm bound serine integrase complex</details>
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                <emdb_id>EMD-47287</emdb_id>
                <relationship>
                    <other>other EM volume</other>
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                <details>cleaved attPmm bound serine integrase and RDF complex in the dimeric state</details>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-47286</emdb_id>
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                    <other>other EM volume</other>
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                <details>attLmm bound serine integrase and RDF complex in the post-rotation state</details>
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                <emdb_id>EMD-47284</emdb_id>
                <relationship>
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                <details>attLmm bound serine integrase and RDF complex in the pre-rotation state</details>
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                <emdb_id>EMD-72632</emdb_id>
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                <details>uncleaved attPsym bound serine integrase and RDF omplex in the dimeric state</details>
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                <emdb_id>EMD-72552</emdb_id>
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                <details>attLsym bound serine integrase complex in the dimeric state</details>
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                <details>attPmm bound serine integrase complex in the tetrameric state</details>
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                <details>attPmm and attBmm bound serine integrase complex in the post-rotation state</details>
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                <details>consensus map of attPmm and attBmm bound integrase complex in the  pre-rotation state</details>
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                <details>attPmm and attBmm bound serine integrase complex in the pre-rotation state</details>
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                <details>cleaved attPmm bound serine integrase and RDF complex in the dimeric state</details>
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                <details>attLmm bound serine integrase and RDF complex in the pre-rotation state</details>
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        <name>attLmm bound serine integrase and RDF complex in the pre-rotation state</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>attLmm bound serine integrase and RDF complex in the pre-rotation state</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="1423">Bacillus subtilis</organism>
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        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Resolvase homolog YokA</name>
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                    <organism ncbi="1423">Bacillus subtilis</organism>
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                <molecular_weight>
                    <theoretical units="MDa">0.06323206599999999</theoretical>
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                <number_of_copies>4</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="866768">Escherichia coli 'BL21-Gold(DE3)pLysS AG'</recombinant_organism>
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                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MELKNIVNSYNITNILGYLRRSRQDMEREKRTGEDTLTEQKELMNKILTAIEIPYELKMEIGSGESIDGRPVFKECLKDL
EEGKYQAIAVKEITRLSRGSYSDAGQIVNLLQSKRLIIITPYKVYDPRNPVDMRQIRFELFMAREEFEMTRERMTGAKYT
YAAQGKWISGLAPYGYQLNKKTSKLDPVEDEAKVVQLIFNIFLNGLNGKDYSYTAIASHLTNLQIPTPSGKKRWNQYTIK
AILQNEVYIGTVKYKVREKTKDGKRTIRPEKEQIVVQDAHAPIIDKEQFQQSQVKIANKVPLLPNKDEFELSELAGVCTC
SKCGEPLSKYESKRIRKNKDGTESVYHVKSLTCKKNKCTYVRYNDVENAILDYLSSLNDLNDSTLTKHINSMLSKYEDDN
SNMKTKKQMSEHLSQKEKELKNKENFIFDKYESGIYSDELFLKRKAALDEEFKELQNAKNELNGLQDTQSEIDSNTVRNN
INKIIDQYHIESSSEKKNELLRMVLKDVIVNMTQKRKGPIPAQFEITPILRFNFIFDLTATNSFH</string>
                    <external_references type="UNIPROTKB">O32006</external_references>
                </sequence>
            </protein_or_peptide>
            <dna macromolecule_id="2">
                <name>DNA (34-MER)</name>
                <natural_source database="NCBI">
                    <organism ncbi="1423">Bacillus subtilis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.010433742</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <sequence>
                    <string>(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DG)(DT)(DA)(DG)(DT)(DA)(DA)(DG)(DT)(DA)(DT)(DC)
(DT)(DT)(DA)(DA)(DT)(DA)(DT)(DA)(DC)(DA)(DG)(DC)(DT)(DT)</string>
                </sequence>
                <classification>DNA</classification>
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            <dna macromolecule_id="3">
                <name>DNA (33-MER)</name>
                <natural_source database="NCBI">
                    <organism ncbi="1423">Bacillus subtilis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.010161636</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <sequence>
                    <string>(DA)(DG)(DC)(DT)(DG)(DT)(DA)(DT)(DA)(DT)(DT)(DA)(DA)(DG)(DA)(DT)(DA)(DC)(DT)(DT)
(DA)(DC)(DT)(DA)(DC)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)</string>
                </sequence>
                <classification>DNA</classification>
            </dna>
            <dna macromolecule_id="4">
                <name>DNA (25-MER)</name>
                <natural_source database="NCBI">
                    <organism ncbi="1423">Bacillus subtilis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.007655984</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <sequence>
                    <string>(DC)(DA)(DT)(DG)(DT)(DC)(DA)(DT)(DT)(DA)(DA)(DT)(DA)(DT)(DC)(DA)(DG)(DT)(DA)(DC)
(DA)(DG)(DA)(DT)(DT)</string>
                </sequence>
                <classification>DNA</classification>
            </dna>
            <dna macromolecule_id="5">
                <name>DNA (24-MER)</name>
                <natural_source database="NCBI">
                    <organism ncbi="1423">Bacillus subtilis</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.0073828010000000005</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <sequence>
                    <string>(DA)(DT)(DC)(DT)(DG)(DT)(DA)(DC)(DT)(DG)(DA)(DT)(DA)(DT)(DT)(DA)(DA)(DT)(DG)(DA)
(DC)(DA)(DT)(DG)</string>
                </sequence>
                <classification>DNA</classification>
            </dna>
            <ligand macromolecule_id="6">
                <name>ZINC ION</name>
                <molecular_weight>
                    <theoretical units="MDa">6.5409e-05</theoretical>
                </molecular_weight>
                <number_of_copies>4</number_of_copies>
                <formula>ZN</formula>
            </ligand>
        </macromolecule_list>
    </sample>
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            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
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                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>8.0</ph>
                    </buffer>
                    <sugar_embedding>
                        <material>vitreous ice</material>
                    </sugar_embedding>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">100</chamber_humidity>
                        <chamber_temperature units="K">291.15</chamber_temperature>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
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                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_defocus_min units="µm">1.0</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.5</nominal_defocus_max>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 (6k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">65.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <ctf_correction>
                    <type>PHASE FLIPPING ONLY</type>
                </ctf_correction>
                <startup_model type_of_model="OTHER"/>
                <final_reconstruction>
                    <resolution units="Å" res_type="BY AUTHOR">4.69</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                        </software>
                    </software_list>
                    <number_images_used>44115</number_images_used>
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                <initial_angle_assignment>
                    <type>OTHER</type>
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                <final_angle_assignment>
                    <type>OTHER</type>
                </final_angle_assignment>
            </singleparticle_processing>
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    </structure_determination_list>
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            <row>453</row>
            <sec>387</sec>
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            <sec>-68</sec>
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            <y>453</y>
            <z>387</z>
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            <b units="Å">482.44504</b>
            <c units="Å">412.15503</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
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            <average>0.0006965972</average>
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            <y units="Å">1.065</y>
            <z units="Å">1.065</z>
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                <level>0.01</level>
                <source>AUTHOR</source>
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