2026-01-14 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation.year _citation_author.identifier_ORCID _em_admin.last_update DATABASE_REFERENCES citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation.year _citation_author.identifier_ORCID _em_admin.last_update RCSB RCSB 2024-10-23 2025-10-08 2025-10-08 2026-01-14 Canadian Institutes of Health Research (CIHR) 175223 Canada Natural Sciences and Engineering Research Council (NSERC, Canada) RGPIN-2022-04723 Canada European Research Council (ERC) 637733 European Union Natural Sciences and Engineering Research Council (NSERC, Canada) RGPIN-2016-04801 Canada Canada Foundation for Innovation 34475 Canada Canadian Institutes of Health Research (CIHR) 377068 Canada Torpedo muscle-type nicotinic acetylcholine receptor - Unliganded State Thompson MJ Nury H Zarkadas E Baenziger JE Nicotinic acetylcholine receptor, Ion channel, Cys-loop receptor, Pentameric ligand-gated ion channel, Neurotransmitter-gated receptor, Ligand-gated ion channel, Primed state, MEMBRANE PROTEIN Thompson MJ Tessier CJG Ananchenko A Henault C Emlaw JR Dehez F Zarkadas E daCosta CJB Nury H Baenziger JE Asynchronous subunit transitions prime acetylcholine receptor activation. Science US 391 eadw1264 eadw1264 2026 41037590 doi:10.1126/science.adw1264 1095-9203 0038 SCIEAS 9e3f FULLOVERLAP EMDB EMD-47481 associated EM volume
Torpedo muscle-type nicotinic acetylcholine receptor - Unliganded State
 Torpedo muscle-type nicotinic acetylcholine receptor Torpedo muscle-type nicotinic acetylcholine receptor 0 1 2 3 4 Tetronarce californica 0.25 Acetylcholine receptor subunit alpha Tetronarce californica 0.050168163999999994 2 LEVO SEHETRLVANLLENYNKVIRPVEHHTHFVDITVGLQLIQLISVDEVNQIVETNVRLRQQWIDVRLRWNPADYGGIKKIRL PSDDVWLPDLVLYNNADGDFAIVHMTKLLLDYTGKIMWTPPAIFKSYCEIIVTHFPFDQQNCTMKLGIWTYDGTKVSISP ESDRPDLSTFMESGEWVMKDYRGWKHWVYYTCCPDTPYLDITYHFIMQRIPLYFVVNVIIPCLLFSFLTGLVFYLPTDSG EKMTLSISVLLSLTVFLLVIVELIPSTSSAVPLIGKYMLFTMIFVISSIIITVVVINTHHRSPSTHTMPQWVRKIFIDTI PNVMFFSTMKRASKEKQENKIFADDIDISDISGKQVTGEVIFQTPLIKNPDVKSAIEGVKYIAEHMKSDEESSNAAEEWK YVAMVIDHILLCVFMLICIIGTVSVFAGRLIELSQEG P02710 Acetylcholine receptor subunit beta Tetronarce californica 0.053731773 1 LEVO SVMEDTLLSVLFETYNPKVRPAQTVGDKVTVRVGLTLTNLLILNEKIEEMTTNVFLNLAWTDYRLQWDPAAYEGIKDLRI PSSDVWQPDIVLMNNNDGSFEITLHVNVLVQHTGAVSWQPSAIYRSSCTIKVMYFPFDWQNCTMVFKSYTYDTSEVTLQH ALDAKGEREVKEIVINKDAFTENGQWSIEHKPSRKNWRSDDPSYEDVTFYLIIQRKPLFYIVYTIIPCILISILAILVFY LPPDAGEKMSLSISALLAVTVFLLLLADKVPETSLSVPIIIRYLMFIMILVAFSVILSVVVLNLHHRSPNTHTMPNWIRQ IFIETLPPFLWIQRPVTTPSPDSKPTIISRANDEYFIRKPAGDFVCPVDNARVAVQPERLFSEMKWHLNGLTQPVTLPQD LKEAVEAIKYIAEQLESASEFDDLKKDWQYVAMVADRLFLYVFFVICSIGTFSIFLDASHNVPPDNPFA P02712 Acetylcholine receptor subunit delta Tetronarce californica 0.057625711 1 LEVO VNEEERLINDLLIVNKYNKHVRPVKHNNEVVNIALSLTLSNLISLKETDETLTSNVWMDHAWYDHRLTWNASEYSDISIL RLPPELVWIPDIVLQNNNDGQYHVAYFCNVLVRPNGYVTWLPPAIFRSSCPINVLYFPFDWQNCSLKFTALNYDANEITM DLMTDTIDGKDYPIEWIIIDPEAFTENGEWEIIHKPAKKNIYPDKFPNGTNYQDVTFYLIIRRKPLFYVINFITPCVLIS FLASLAFYLPAESGEKMSTAISVLLAQAVFLLLTSQRLPETALAVPLIGKYLMFIMSLVTGVIVNCGIVLNFHFRTPSTH VLSTRVKQIFLEKLPRILHMSRADESEQPDWQNDLKLRRSSSVGYISKAQEYFNIKSRSELMFEKQSERHGLVPRVTPRI GFGNNNENIAASDQLHDEIKSGIDSTNYIVKQIKEKNAYDEEVGNWNLVGQTIDRLSMFIITPVMVLGTIFIFVMGNFNH PPAKPFEGDPFDYSSDHPRCA P02718 Acetylcholine receptor subunit gamma Tetronarce californica 0.056206573999999995 1 LEVO NEEGRLIEKLLGDYDKRIIPAKTLDHIIDVTLKLTLTNLISLNEKEEALTTNVWIEIQWNDYRLSWNTSEYEGIDLVRIP SELLWLPDVVLENNVDGQFEVAYYANVLVYNDGSMYWLPPAIYRSTCPIAVTYFPFDWQNCSLVFRSQTYNAHEVNLQLS AEEGEAVEWIHIDPEDFTENGEWTIRHRPAKKNYNWQLTKDDTDFQEIIFFLIIQRKPLFYIINIIAPCVLISSLVVLVY FLPAQAGGQKCTLSISVLLAQTIFLFLIAQKVPETSLNVPLIGKYLIFVMFVSMLIVMNCVIVLNVSLRTPNTHSLSEKI KHLFLGFLPKYLGMQLEPSEETPEKPQPRRRSSFGIMIKAEEYILKKPRSELMFEEQKDRHGLKRVNKMTSDIDIGTTVD LYKDLANFAPEIKSCVEACNFIAKSTKEQNDSGSENENWVLIGKVIDKACFWIALLLFSIGTLAIFLTGHFNQVPEFPFP GDPRKYVP P02714 (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate 0.0007600759999999999 8 POV singleParticle particle 0.65 7.4 25.0 Tris-HCl Tris(hydroxymethyl)aminomethane hydrochloride 125.0 NaCl sodium chloride Quantifoil R1.2/1.3 GOLD CARBON HOLEY GLOW DISCHARGE ETHANE 100 277 FEI VITROBOT MARK IV
Sample was monodisperce but suffered from orientation bias.
 TFS GLACIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.7 0.7000000000000001 2.2 NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 40.0 1 664005 CTFFIND 4.0 PHASE FLIPPING AND AMPLITUDE CORRECTION 7QKO C1 FOURIER SPACE 3.2 FSC 0.143 CUT-OFF cryoSPARC 4.0 169980 MAXIMUM LIKELIHOOD cryoSPARC 4.0 MAXIMUM LIKELIHOOD cryoSPARC 4.0 emd_47481.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 293.12 293.12 293.12 90.0 90.0 90.0 X Y Z -0.29051763 0.74551225 0.0001438959 0.024917496 1.145 1.145 1.145 0.2 AUTHOR Unsharpened map emd_47481_msk_1.map emd_47481_msk_2.map emd_47481_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 293.12 293.12 293.12 90.0 90.0 90.0 X Y Z -0.016513411 1.6943891 0.001619499 0.028440025 1.145 1.145 1.145 AUTHOR DeepEM sharpended map emd_47481_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 293.12 293.12 293.12 90.0 90.0 90.0 X Y Z -0.7644182 0.88507754 0.0010493026 0.08319194 1.145 1.145 1.145 AUTHOR Half map B emd_47481_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 293.12 293.12 293.12 90.0 90.0 90.0 X Y Z -0.63203996 0.9140398 0.0010451619 0.083184 1.145 1.145 1.145 AUTHOR Half map A