Human ASIC1a at pH 5.7 with domain-swapped transmembrane domain

2026-01-21 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2024-10-24 2026-01-21 2026-01-21 2026-01-21 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R01GM138862 United States Human ASIC1a at pH 5.7 with domain-swapped transmembrane domain Hartfield KA Cahill J Baconguis I Ion channel, Trimer, MEMBRANE PROTEIN Cahill J Hartfield KA Heusser SA Ritter N Poulsen MH Yoshioka C Pless SA Baconguis I Conformational plasticity of human acid-sensing ion channel 1a To Be Published 0353 9e4f FULLOVERLAP EMDB EMD-47508 associated EM volume

Homotrimeric complex of acid-sensing channel 1a Homotrimeric complex of acid-sensing channel 1a 0 1 Homo sapiens Acid-sensing ion channel 1 Homo sapiens 0.060252625

Thrombin cleavage product of construct with N-terminal fusion of 8xHis-EGFP-Thrombin site

3 Homo sapiens LEVO GGRMELKAEEEEVGGVQPVSIQAFASSSTLHGLAHIFSYERLSLKRALWALCFLGSLAVLLCVCTERVQYYFHYHHVTKL DEVAASQLTFPAVTLCNLNEFRFSQVSKNDLYHAGELLALLNNRYEIPDTQMADEKQLEILQDKANFRSFKPKPFNMREF YDRAGHDIRDMLLSCHFRGEVCSAEDFKVVFTRYGKCYTFNSGRDGRPRLKTMKGGTGNGLEIMLDIQQDEYLPVWGETD ETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVACQEQRLIYLPPPWGTCKAVTMDSDLDFFDSYSITACRIDCETR YLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKS EQYIGENILVLDIFFEVLNYETIEQKKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHKLCRRGKCQKEAKR SSADKGVALSLDDVKRHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC P78348 singleParticle particle 4 5.7 50.0 Na2HPO4/NaH2PO4 sodium phosphate 200.0 NaCl sodium chloride 1.0 digitonin 1.0 C9H15O6P Tris(2-carboxyethyl)phosphine hydrochloride Quantifoil R2/1 GOLD 200 CARBON HOLEY GLOW DISCHARGE 30 ETHANE 100 285.15 FEI VITROBOT MARK IV

Monodisperse sample of homotrimeric ASIC1a

TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.01 0.8 2.5 GATAN K3 BIOCONTINUUM (6k x 4k) 65.0 1 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION C3 2.69 FSC 0.143 CUT-OFF cryoSPARC 54398 MAXIMUM LIKELIHOOD cryoSPARC MAXIMUM LIKELIHOOD cryoSPARC emd_47508.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 322.176 322.176 322.176 90.0 90.0 90.0 X Y Z -0.2909437 0.7033403 0.00044178206 0.014464637 0.839 0.839 0.839 0.11 AUTHOR ::::EMDATABANK.org::::EMD-47508:::: Map of human ASIC1a at pH 5.7, Domain-swapped, Desensitized emd_47508_msk_1.map emd_47508_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 322.176 322.176 322.176 90.0 90.0 90.0 X Y Z -0.77260035 0.92255366 0.00056005607 0.0907779 0.839 0.839 0.839 AUTHOR ::::EMDATABANK.org::::EMD-47508:::: Half map B corresponding to the primary map emd_47508_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 322.176 322.176 322.176 90.0 90.0 90.0 X Y Z -0.81033117 0.91308224 0.00057573 0.090772316 0.839 0.839 0.839 AUTHOR ::::EMDATABANK.org::::EMD-47508:::: Half map B corresponding to the primary map