Montaged cryo-ET map of individual particle 3D reconstructions of ~2 kb negatively supercoiled plasmids (delta Lk centered on -15) under near physiological salt condition (40 mM K+, 5 mM Mg2+)

2026-03-18 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2024-11-11 2026-03-18 2026-03-18 2026-03-18 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) United States Montaged cryo-ET map of individual particle 3D reconstructions of ~2 kb negatively supercoiled plasmids (delta Lk centered on -15) under near physiological salt condition (40 mM K+, 5 mM Mg2+) Zhang M Bustamante C DNA Supercoiling, Transcription regulation, Cryo-Electron Tomography (cryo-ET), Topological Domains, DNA Zhang M Bustamante C Interplay Between DNA Supercoiling and RNA Polymerase Localization at Plectoneme Apices Modulate Transcription Dynamics To Be Published 0353 EMDB EMD-47844 associated EM volume 2 kb negatively supercoiled pUC19 plasmids containing a T7A1-U promoter 2 kb negatively supercoiled pUC19 plasmids containing a T7A1-U promoter 0 Escherichia coli

pUC19 plasmid

tomography filament 7.4 ETHANE 95 288 LEICA EM GP NO SECTIONING TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 2.0 4.0 53000.0 NITROGEN GATAN K3 (6k x 4k) 130.0 1 EMAN2 SPIDER 35 EMAN2 PHASE FLIPPING ONLY emd_47844.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 1200 1000 200 0 0 0 1200 1000 200 14027.999 11690.0 2338.0 90.0 90.0 90.0 X Y Z -0.5162568 4.5810475 0.0009016721 0.031435337 11.69 11.69 11.69 AUTHOR ::::EMDATABANK.org::::EMD-47844:::: Montaged cryo-ET map of individual particle 3D reconstructions of ~2 kb negatively supercoiled plasmids (delta Lk centered on -15) under near physiological salt condition (40 mM K , 5 mM Mg2 )