2026-04-15 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.pdbx_database_id_PubMed _citation.title _citation_author.name _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.pdbx_database_id_PubMed _citation.title _citation_author.name _em_admin.last_update DATABASE_REFERENCES citation citation_author em_admin _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _citation_author.identifier_ORCID _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _citation_author.identifier_ORCID _em_admin.last_update RCSB RCSB 2024-12-10 2025-12-17 2025-12-17 2026-04-15 National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R01AI081059 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) U19AI158028 United States beta-barrel assembly machine from Escherichia coli in a late state of substrate assembly Thomson BD Kahne D beta-barrel assembly machine, outer membrane, folding intermediate, MEMBRANE PROTEIN Thomson BD Marquez MD Rawson S Dos Santos TMA Harrison SC Kahne D Structures of folding intermediates on BAM show diverse substrates fold by a conserved mechanism. Proc.Natl.Acad.Sci.USA US 123 e2534936123 e2534936123 2026 41926538 doi:10.1073/pnas.2534936123 1091-6490 0040 PNASA6 EMD-48251 consensus EM volume EMD-48252 focused EM volume 9mgg FULLOVERLAP EMDB EMD-48251 consensus EM volume EMDB EMD-48252 focused EM volume EMDB EMD-48255 associated EM volume
beta-barrel assembly machine from Escherichia coli in an late state of substrate assembly
 BamABCDE bound to BamA substrate in a late folding intermediate state BamABCDE bound to BamA substrate in a late folding intermediate state 0 1 2 3 4 5 6 Escherichia coli K-12 Outer membrane protein assembly factor BamA Escherichia coli K-12 0.089607969 1 Escherichia coli BL21(DE3) LEVO AEGHHHHHHHHFVVKDIHFEGLQRVAVGAALLSMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERP TIASITFSGNKSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPRNRVDLKLVFQEG VSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQKLAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKK GIYVTVNITEGDQYKLSGVEVSGNLAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDAD KTVKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRLGFFETVDTDTQRVPGSPDQV DVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWLGTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYN DFQADDADLSDYTNKSYGTCVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFKTDDFTF NYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDDHKWVVLGRTRWGYGDGLGGKEMPFYENF YAGGSSTVRGFQSNTIGPKAVYFPHQASNYDPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANS VRTSFFWDMGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDKAEQFQFNIGKTW P0A940 Outer membrane protein assembly factor BamC Escherichia coli K-12 0.036875277 1 Escherichia coli BL21(DE3) LEVO MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEAAPLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKAL DIRPPAQPLALVSGARTQFTGDTASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAANAAQNRASTTMDVQSAADDTGLP MLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNMAVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFID PKGHTLTQSQNDALVAVFQAAFSK P0A903 Outer membrane protein assembly factor BamD Escherichia coli K-12 0.027858349999999997 1 Escherichia coli BL21(DE3) LEVO MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQDGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYY KNADLPLAQAAIDRFIRLNPTHPNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAA NSSNT P0AC02 Outer membrane protein assembly factor BamE Escherichia coli K-12 0.012310977 1 Escherichia coli BL21(DE3) LEVO MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVSKIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQ PGHEGVTQQTLTLTFNSSGVLTNIDNKPALSGN P0A937 Outer membrane protein assembly factor BamB Escherichia coli K-12 0.041918945 1 Escherichia coli BL21(DE3) LEVO MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTPTTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGL VKALNADDGKEIWSVSLAEKDGWFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPTTAFGAAVVGGDNGRVSAVLMEQGQMIWQQRISQATGSTE IDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQIMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQS DLLHRLLTSPVLYNGNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR P77774 Outer membrane protein assembly factor BamA Escherichia coli K-12 0.062852711 1 Escherichia coli BL21(DE3) LEVO AWSHPQFEKGGGSGGGSGGSAWSHPQFEKEGFVVKDIHFEGLQRVAVGAALLSMPVRTGDTVNDEDISNTIRALFATGNF EDVRVLRDGDTLLVQVKERPTIASITFSGNKSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVK AVVTPLPRNRVDLKLVFQENTGSFNFGIGYGTESGVSFQAGVQQDNWLGTGYAVGCNGTKNDYQTYAELSVTNPYFTVDG VSLGGRLFYNDFQADDADLSDYTNKSYGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQD NSFKTDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDDHKWVVLGRTRWGYGDGLG GKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNYDPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPT PFISDKYANSVRTSFFWDMGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDKAEQF QFNIGKTW P0A940 P0A940 singleParticle particle 1 8.0 20.0 Tris-HCl Tris(hydroxymethyl)aminomethane hydrochloride 150.0 NaCl Sodium chloride 0.004 GDN Glyco-diosgenin UltrAuFoil R1.2/1.3 GOLD 300 GOLD GLOW DISCHARGE AIR 0.039 ETHANE 100 277 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 1.0 2.4 105000.0 GATAN K3 BIOQUANTUM (6k x 4k) 1 21962 2.5 49.6 1 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION
Ab-initio model generated within cryoSPARC based on the data
 3.9 FSC 0.143 CUT-OFF cryoSPARC 39744 RANDOM ASSIGNMENT cryoSPARC MAXIMUM LIKELIHOOD cryoSPARC emd_48255.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 265.6 265.6 265.6 90.0 90.0 90.0 Z Y X -23.844887 34.842711999999999 0.0059813005 1.0154202 0.83000004 0.83000004 0.83000004 5.4 AUTHOR Composite map 5D0O PDB experimental model 5D0Q PDB experimental model