2025-12-24 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2024-12-19 2025-12-24 2025-12-24 2025-12-24 Richard and Susan Smith Family Foundation United States Damon Runyon Cancer Research Foundation United States National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS) DP2-ES036404 United States Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome CTR9 TPR local map L Syau D Farnung L polymerase, elongation factor, nucleosome, TRANSCRIPTION Syau D Farnung L Structure and function of IWS1 in transcription elongation To Be Published 0353 PDB 9MLC unspecified
9MLC contains the model for the composite map associated with this entry
 EMDB EMD-48366 associated EM volume RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome complex RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome complex 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Homo sapiens 1.7 RNA polymerase II 1 Sus scrofa SPT6 1 Homo sapiens DSIF (SPT4 and SPT5) 1 Homo sapiens PAF1 complex (CTR9, CDC73, PAF1, LEO1, WDR61, RTF1) 1 Homo sapiens PAF1 complex (CTR9, CDC73, PAF1, LEO1, WDR61) 5
CTR9, CDC73, PAF1, LEO1, WDR61 expressed and purified together
Homo sapiens RTF1 5
RTF1 expressed and purified separately
Homo sapiens TFIIS 1 Homo sapiens IWS1 1 Homo sapiens ELOF1 1 Homo sapiens LEDGF 1 Homo sapiens Nucleosome 1
Nucleosome containing H3K36cme3-modified histone octamer and synthetic DNA
Xenopus laevis singleParticle particle 7.4 20.0 HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid 50.0 NaCl sodium chloride 3.0 MgCl2 magnesium chloride 1.0 TCEP (tris(2-carboxyethyl)phosphine) ETHANE 100 277 FEI VITROBOT MARK IV TFS KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 0.6 1.8 105000.0 VOLTA PHASE PLATE TFS Selectris TFS FALCON 4i (4k x 4k) 2 30316 4.21 37.0 1 4597368 cryoSPARC 3.2.0 PHASE FLIPPING AND AMPLITUDE CORRECTION 2.6 FSC 0.143 CUT-OFF cryoSPARC 4.7.0 63837 MAXIMUM LIKELIHOOD cryoSPARC 3.2.0 MAXIMUM LIKELIHOOD cryoSPARC 3.2.0 emd_48366.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 460 460 460 0 0 0 460 460 460 547.4 547.4 547.4 90.0 90.0 90.0 X Y Z -0.15196584 0.36762065 -0.0002481941 0.00668384 1.19 1.19 1.19 0.05 AUTHOR Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome CTR9 TPR local map L emd_48366_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 460 460 460 0 0 0 460 460 460 547.4 547.4 547.4 90.0 90.0 90.0 X Y Z -0.18389642 0.42397645 0.00021222532 0.018991124 1.19 1.19 1.19 AUTHOR Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome CTR9 TPR local map L half A emd_48366_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 460 460 460 0 0 0 460 460 460 547.4 547.4 547.4 90.0 90.0 90.0 X Y Z -0.18034609 0.41970402 0.00021098697 0.018909618 1.19 1.19 1.19 AUTHOR Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome CTR9 TPR local map L half B