Structure of AG2-G02 Fab in complex with influenza H3N8 A/Mallard/Alberta/362/2017 Hemagglutinin

2025-10-08 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-02-02 2025-10-08 2025-10-08 2025-10-08 National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R01 AI167910 United States Structure of AG2-G02 Fab in complex with influenza H3N8 A/Mallard/Alberta/362/2017 Hemagglutinin Gopal AB Wu NC Lv H Pholcharee T Influenza, Antibody, Immune system, Hemagglutinin, VIRAL PROTEIN-IMMUNE SYSTEM complex Gopal AB Wu NC Lv H Pholcharee T Structure of AG2-G02 Fab in complex with influenza H3N8 A/Mallard/Alberta/362/2017 Hemagglutinin To Be Published 0353 9n4e FULLOVERLAP EMDB EMD-48873 associated EM volume

AG2-G02 Fab in complex with influenza H3N8 A/Mallard/Alberta/362/2017 hemagglutinin AG2-G02 Fab in complex with influenza H3N8 A/Mallard/Alberta/362/2017 hemagglutinin 0 1 2 3

Hemagglutinin was expressed recombinantly in SF9 cells. AG2-G02 Fab was expressed recombinantly in Expi293F cells.

Homo sapiens 0.42 Hemagglutinin Influenza A virus A/Mallard/Alberta/362/2017 0.055422828 3 Spodoptera frugiperda LEVO ATLCLGHHAVPNGTIVKTITDDQIEVTNATELVQSSSTGKICNNPHRILDGRDCTLIDALLGDPHCDVFQDETWDLYVER SSAFSNCYPYDVPDYASLRSLVASSGTLEFITEGFTWTGVTQNGGSNACKRGPASGFFSRLNWLTKSGSAYPVLNVTMPN NDNFDKLYVWGVHHPSTNQEQTSLYVQASGRVTVSTRRSQQTIIPNIGSRPWVRGQSGRISIYWTIVKPGDVLVINSNGN LIAPRGYFKMRTGKSSIMRSDAPIDTCISECITPNGSIPNDKPFQNVNKITYGACPKYVKQSTLKLATGMRNVPEKQTRG LFGAKAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEKFHQIEKEFSEVEGRIQDLE KYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTRRQLRENAEDMGNGCFKIYHKCDNACIESIRNGTYDHDI YRDEALNNRFQIKGV A0A2Z4WHK0 Variable domain of the heavy chain of Fab AG2-G02 Homo sapiens 0.013807327999999999 3 Homo sapiens LEVO VQLVQSGAEMKKPGASVKVSCKASGYTFSAYYIHWVRQAPGQGLEWMGRINTDSGGTNFAQQFEGRVTMTRDTSISTAYM ELSSLRSDDTAVYYCARGYDFWLGSYRAGGWFDPWGQGTLVTVSS Variable domain of the light chain of Fab AG2-G02 Homo sapiens 0.011858150999999999 3 Homo sapiens LEVO EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFRGSGSGTDFTLTISRLE PEDFAVYYCQQYGSSPQGFTFGPGTKVDIK Hemagglutinin Influenza A virus A/Mallard/Alberta/362/2017 0.055449851999999994 3 Spodoptera frugiperda LEVO ATLCLGHHAVPNGTIVKTITDDQIEVTNATELVQSSSTGKICNNPHRILDGRDCTLIDALLGDPHCDVFQDETWDLYVER SSAFSNCYPYDVPDYASLRSLVASSGTLEFITEGFTWTGVTQNGGSNACKRGPASGFFSRLNWLTKSGSAYPVLNVTMPN NDNFDKLYVWGVHHPSTNQEQTNLYVQASGRVTVSTRRSQQTIIPNIGSRPWVRGQSGRISIYWTIVKPGDVLVINSNGN LIAPRGYFKMRTGKSSIMRSDAPIDTCISECITPNGSIPNDKPFQNVNKITYGACPKYVKQSTLKLATGMRNVPEKQTRG LFGAKAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEKFHQIEKEFSEVEGRIQDLE KYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTRRQLRENAEDMGNGCFKIYHKCDNACIESIRNGTYDHDI YRDEALNNRFQIKGV A0A2Z4WF96 singleParticle particle 1 8.0

20 mM Tris-HCl, 100 mM NaCl, 10 mM CaCl2, pH 8.0

Quantifoil R1.2/1.3 COPPER 300 GLOW DISCHARGE ETHANE 90 277.15 FEI VITROBOT MARK III

The sample was also mixed with octyl glucoside to 0.1% w/v of the detergent final concentration prior to applying to the grid.

TFS KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.5 3.0 GATAN K3 (6k x 4k) 57.35 1 NONE

Initial model was built directly into the map using automated model building algorithm ModelAngelo (DOI: 10.1038/s41586-024-07215-4)

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