2026-02-11 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-02-04 2026-02-11 2026-02-11 2026-02-11 National Institutes of Health/National Cancer Institute (NIH/NCI) ZIA BC 011627 United States Cryo-ET of FIB-milled synapse region between CD19+ NALM6 leukemia cells and T-cells expressing 8H47 CD19 CAR construct Chen X Walters KJ CAR T-cells, Hinge design, Immune Synapse, IMMUNE SYSTEM Mirazee J Aranganathan A Chen X Tiwary P Ding J Mizuno N Walters KJ Taylor N Gregoire AB CAR-BOOSTing T-Cell Responsiveness through Synaptic Cleft Engineering To Be Published 0353 EMDB EMD-48920 associated EM volume cryo-FIB milled synapse between CD19+ NALM6 leukemia cells and T-cells expressing 8H47 CD19 CAR construct cryo-FIB milled synapse between CD19+ NALM6 leukemia cells and T-cells expressing 8H47 CD19 CAR construct 0 Homo sapiens tomography cell 7.4
RPMI (Thermo Fisher Scientific) supplemented with 8.9% FBS, Glutamax, and penicillin/streptomycin
 Quantifoil R1.2/1.3 COPPER 300 GLOW DISCHARGE AIR
20 mA
 ETHANE 80 303 LEICA EM GP OTHER OTHER 30 0.03 3600 80 1000 200
The value given for _em_focused_ion_beam.instrument is Aquilos 2 FIB-SEM. This is not in a list of allowed values {'OTHER', 'DB235'} so OTHER is written into the XML file.
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 3.0 4.0 26000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K3 BIOQUANTUM (6k x 4k) 5760 4092 61 1.35 1 BACK PROJECTION 6.69 IMOD 4.11.25 34 CTFFIND NONE emd_48920.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 2014 2638 518 0 0 0 2014 2638 518 13463.59 17635.03 3462.83 90.0 90.0 90.0 X Y Z -166.913060000000002 232.205500000000001 -0.000000001351517 22.720879 6.685 6.685 6.685 AUTHOR Lamella2_Position2