2025-12-31 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation_author.identifier_ORCID _em_admin.last_update DATABASE_REFERENCES citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation_author.identifier_ORCID _em_admin.last_update RCSB RCSB 2025-02-05 2025-11-05 2025-11-05 2025-12-31 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) 5R01GM145950-03 United States Chan Zuckerberg Initiative 2023-332391 United States SSU processome maturation and disassembly, State N Buzovetsky O Klinge S SSU processome, ribosome assembly, RNA folding, RNA-protein interactions, RIBOSOME Buzovetsky O Klinge S Helicase-mediated mechanism of SSU processome maturation and disassembly. Nature UK 648 746 754 2025 41162712 doi:10.1038/s41586-025-09688-3 1476-4687 0006 NATUAS EMD-49029 consensus EM volume
Overall
 EMD-49030 focused EM volume
Core
 EMD-49031 focused EM volume
Dhr1
 EMD-49032 focused EM volume
Utp20
 EMD-49033 focused EM volume
Noc4Nop14
 9n7a FULLOVERLAP EMDB EMD-49029 consensus EM volume
Overall
 EMDB EMD-49030 focused EM volume
Core
 EMDB EMD-49031 focused EM volume
Dhr1
 EMDB EMD-49032 focused EM volume
Utp20
 EMDB EMD-49033 focused EM volume
Noc4Nop14
 EMDB EMD-49090 associated EM volume
SSU processome maturation and disassembly, State N
 SSU processome maturation and disassembly, State N SSU processome maturation and disassembly, State N 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 Saccharomyces cerevisiae 18S rRNA Saccharomyces cerevisiae S288C 0.580765688 1 UAUCUGGUUGAUCCUGCCAGUAGUCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUAUAAGCAAUUUAUACA GUGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUCGUUUAUUUGAUAGUUCCUUUACUACAUGGUAUAACUGUGGUAAUUC UAGAGCUAAUACAUGCUUAAAAUCUCGACCCUUUGGAAGAGAUGUAUUUAUUAGAUAAAAAAUCAAUGUCUUCGGACUCU UUGAUGAUUCAUAAUAACUUUUCGAAUCGCAUGGCCUUGUGCUGGCGAUGGUUCAUUCAAAUUUCUGCCCUAUCAACUUU CGAUGGUAGGAUAGUGGCCUACCAUGGUUUCAACGGGUAACGGGGAAUAAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAA ACGGCUACCACAUCCAAGGAAGGCAGCAGGCGCGCAAAUUACCCAAUCCUAAUUCAGGGAGGUAGUGACAAUAAAUAACG AUACAGGGCCCAUUCGGGUCUUGUAAUUGGAAUGAGUACAAUGUAAAUACCUUAACGAGGAACAAUUGGAGGGCAAGUCU GGUGCCAGCAGCCGCGGUAAUUCCAGCUCCAAUAGCGUAUAUUAAAGUUGUUGCAGUUAAAAAGCUCGUAGUUGAACUUU GGGCCCGGUUGGCCGGUCCGAUUUUUUCGUGUACUGGAUUUCCAACGGGGCCUUUCCUUCUGGCUAACCUUGAGUCCUUG UGGCUCUUGGCGAACCAGGACUUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCGUAUUGCUCGAAUAUAUUAGCAU GGAAUAAUAGAAUAGGACGUUUGGUUCUAUUUUGUUGGUUUCUAGGACCAUCGUAAUGAUUAAUAGGGACGGUCGGGGGC AUCAGUAUUCAAUUGUCAGAGGUGAAAUUCUUGGAUUUAUUGAAGACUAACUACUGCGAAAGCAUUUGCCAAGGACGUUU UCAUUAAUCAAGAACGAAAGUUAGGGGAUCGAAGAUGAUCAGAUACCGUCGUAGUCUUAACCAUAAACUAUGCCGACUAG GGAUCGGGUGGUGUUUUUUUAAUGACCCACUCGGCACCUUACGAGAAAUCAAAGUCUUUGGGUUCUGGGGGGAGUAUGGU CGCAAGGCUGAAACUUAAAGGAAUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGG GAAACUCACCAGGUCCAGACACAAUAAGGAUUGACAGAUUGAGAGCUCUUUCUUGAUUUUGUGGGUGGUGGUGCAUGGCC GUUCUUAGUUGGUGGAGUGAUUUGUCUGCUUAAUUGCGAUAACGAACGAGACCUUAACCUACUAAAUAGUGGUGCUAGCA UUUGCUGGUUAUCCACUUCUUAGAGGGACUAUCGGUUUCAAGCCGAUGGAAGUUUGAGGCAAUAACAGGUCUGUGAUGCC CUUAGACGUUCUGGGCCGCACGCGCGCUACACUGACGGAGCCAGCGAGUCUAACCUUGGCCGAGAGGUCUUGGUAAUCUU GUGAAACUCCGUCGUGCUGGGGAUAGAGCAUUGUAAUUAUUGCUCUUCAACGAGGAAUUCCUAGUAAGCGCAAGUCAUCA GCUUGCGUUGAUUACGUCCCUGCCCUUUGUACACACCGCCCGUCGCUAGUACCGAUUGAAUGGCUUAGUGAGGCCUCAGG AUCUGCUUAGAGAAGGGGGCAACUCCAUCUCAGAGCGGAGAAUUUGGACAAACUUGGUCAUUUAGAGGAACUAAAAGUCG UAACAAGGUUUCCGUAGGUGAACCUGCGGAAGGAUCAUUAAAG U53879.1 U3 snoRNA Saccharomyces cerevisiae S288C 0.106943469 1 (M7G)GUCGACGUACUUCAUAGGAUCAUUUCUAUAGGAAUCGUCACUCUUUGACUCUUCAAAAGAGCCACUGAAUCCAAC UUGGUUGAUGAGUCCCAUAACCUUUGUACCCCAGAGUGAGAAACCGAAAUUGAAUCUAAAUUAGCUUGGUCCGCAAUCCU UAGCGGUUCGGCCAUCUAUAAUUUUGAAUAAAAAUUUUGCUUUGCCGUUGCAUUUGUAGUUUUUUCCUUUGGAAGUAAUU ACAAUAUUUUAUGGCGCGAUGAUCUUGACCCAUCCUAUGUACUUCUUUUUUGAAGGGAUAGGGCUCUAUGGGUGGGUACA AAUGGCAGUCUGACAAGU 40S ribosomal protein S18-A Saccharomyces cerevisiae S288C 0.017071641 1 LEVO MSLVVQEQGSFQHILRLLNTNVDGNIKIVYALTTIKGVGRRYSNLVCKKADVDLHKRAGELTQEELERIVQIMQNPTHYK IPAWFLNRQNDITDGKDYHTLANNVESKLRDDLERLKKIRAHRGIRHFWGLRVRGQHTKTTGRRRA P0CX55 40S ribosomal protein S4-A Saccharomyces cerevisiae S288C 0.029469330000000002 1 LEVO MARGPKKHLKRLAAPHHWLLDKLSGCYAPRPSAGPHKLRESLPLIVFLRNRLKYALNGREVKAILMQRHVKVDGKVRTDT TYPAGFMDVITLDATNENFRLVYDVKGRFAVHRITDEEASYKLGKVKKVQLGKKGVPYVVTHDGRTIRYPDPNIKVNDTV KIDLASGKITDFIKFDAGKLVYVTGGRNLGRIGTIVHKERHDGGFDLVHIKDSLDNTFVTRLNNVFVIGEQGKPYISLPK GKGIKLSIAEERDRRRAQQGL P0CX35 40S ribosomal protein S5 Saccharomyces cerevisiae S288C 0.025072599999999997 1 LEVO MSDTEAPVEVQEDFEVVEEFTPVVLATPIPEEVQQAQTEIKLFNKWSFEEVEVKDASLVDYVQVRQPIFVAHTAGRYANK RFRKAQCPIIERLTNSLMMNGRNNGKKLKAVRIIKHTLDIINVLTDQNPIQVVVDAITNTGPREDTTRVGGGGAARRQAV DVSPLRRVNQAIALLTIGAREAAFRNIKTIAETLAEELINAAKGSSTSYAIKKKDELERVAKSNR P26783 40S ribosomal protein S6-A Saccharomyces cerevisiae S288C 0.027054486 1 LEVO MKLNISYPVNGSQKTFEIDDEHRIRVFFDKRIGQEVDGEAVGDEFKGYVFKISGGNDKQGFPMKQGVLLPTRIKLLLTKN VSCYRPRRDGERKRKSVRGAIVGPDLAVLALVIVKKGEQELEGLTDTTVPKRLGPKRANNIRKFFGLSKEDDVRDFVIRR EVTKGEKTYTKAPKIQRLVTPQRLQRKRHQRALKVRNAQAQREAAAEYAQLLAKRLSERKAEKAEIRKRRASSLKA P0CX37 40S ribosomal protein S7-A Saccharomyces cerevisiae S288C 0.021658208999999998 1 LEVO MSAPQAKILSQAPTELELQVAQAFVELENSSPELKAELRPLQFKSIREIDVAGGKKALAIFVPVPSLAGFHKVQTKLTRE LEKKFQDRHVIFLAERRILPKPSRTSRQVQKRPRSRTLTAVHDKILEDLVFPTEIVGKRVRYLVGGNKIQKVLLDSKDVQ QIDYKLESFQAVYNKLTGKQIVFEIPSETH P26786 40S ribosomal protein S8-A Saccharomyces cerevisiae S288C 0.022537803 1 LEVO MGISRDSRHKRSATGAKRAQFRKKRKFELGRQPANTKIGAKRIHSVRTRGGNKKYRALRIETGNFSWASEGISKKTRIAG VVYHPSNNELVRTNTLTKAAIVQIDATPFRQWFEAHYGQTLGKKKNVKEEETVAKSKNAERKWAARAASAKIESSVESQF SAGRLYACISSRPGQSGRCDGYILEGEELAFYLRRLTAKK P0CX39 40S ribosomal protein S9-A Saccharomyces cerevisiae S288C 0.022487893 1 LEVO MPRAPRTYSKTYSTPKRPYESSRLDAELKLAGEFGLKNKKEIYRISFQLSKIRRAARDLLTRDEKDPKRLFEGNALIRRL VRVGVLSEDKKKLDYVLALKVEDFLERRLQTQVYKLGLAKSVHHARVLITQRHIAVGKQIVNIPSFMVRLDSEKHIDFAP TSPFGGARPGRVARRNAARKAEASGEAADEADEADEE O13516 40S ribosomal protein S16-A Saccharomyces cerevisiae S288C 0.015877489999999998 1 LEVO MSAVPSVQTFGKKKSATAVAHVKAGKGLIKVNGSPITLVEPEILRFKVYEPLLLVGLDKFSNIDIRVRVTGGGHVSQVYA IRQAIAKGLVAYHQKYVDEQSKNELKKAFTSYDRTLLIADSRRPEPKKFGGKGARSRFQKSYR P0CX51 40S ribosomal protein S11-A Saccharomyces cerevisiae S288C 0.017785934 1 LEVO MSTELTVQSERAFQKQPHIFNNPKVKTSKRTKRWYKNAGLGFKTPKTAIEGSYIDKKCPFTGLVSIRGKILTGTVVSTKM HRTIVIRRAYLHYIPKYNRYEKRHKNVPVHVSPAFRVQVGDIVTVGQCRPISKTVRFNVVKVSAAAGKANKQFAKF P0CX47 40S ribosomal protein S22-A Saccharomyces cerevisiae S288C 0.014650061999999998 1 LEVO MTRSSVLADALNAINNAEKTGKRQVLIRPSSKVIIKFLQVMQKHGYIGEFEYIDDHRSGKIVVQLNGRLNKCGVISPRFN VKIGDIEKWTANLLPARQFGYVILTTSAGIMDHEEARRKHVSGKILGFVY P0C0W1 40S ribosomal protein S24-A Saccharomyces cerevisiae S288C 0.015362847999999998 1 LEVO MSDAVTIRTRKVISNPLLARKQFVVDVLHPNRANVSKDELREKLAEVYKAEKDAVSVFGFRTQFGGGKSVGFGLVYNSVA EAKKFEPTYRLVRYGLAEKVEKASRQQRKQKKNRDKKIFGTGKRLAKKVARRNAD P0CX31 40S ribosomal protein S28-A Saccharomyces cerevisiae S288C 0.007605847 1 LEVO MDNKTPVTLAKVIKVLGRTGSRGGVTQVRVEFLEDTSRTIVRNVKGPVRENDILVLMESEREARRLR Q3E7X9 U3snoRNA segment Saccharomyces cerevisiae S288C 0.001485872 1 UUUUU U3 small nucleolar RNA-associated protein MPP10 Saccharomyces cerevisiae S288C 0.067042492 1 LEVO MSELFGVLKSNAGRIILKDPSATSKDVKAYIDSVINTCKNGSITKKAELDEITVDGLDANQVWWQVKLVLDSIDGDLIQG IQELKDVVTPSHNLSDGSTLNSSSGEESELEEAESVFKEKQMLSADVSEIEEQSNDSLSENDEEPSMDDEKTSAEAAREE FAEEKRISSGQDERHSSPDPYGINDKFFDLEKFNRDTLAAEDSNEASEGSEDEDIDYFQDMPSDDEEEEAIYYEDFFDKP TKEPVKKHSDVKDPKEDEELDEEEHDSAMDKVKLDLFADEEDEPNAEGVGEASDKNLSSFEKQQIEIRKQIEQLENEAVA EKKWSLKGEVKAKDRPEDALLTEELEFDRTAKPVPVITSEVTESLEDMIRRRIQDSNFDDLQRRTLLDITRKSQRPQFEL SDVKSSKSLAEIYEDDYTRAEDESALSEELQKAHSEISELYANLVYKLDVLSSVHFVPKPASTSLEIRVETPTISMEDAQ PLYMSNASSLAPQEIYNVGKAEKDGEIRLKNGVAMSKEELTREDKNRLRRALKRKRSKANLPNVNKRSKRNDVVDTLSKA KNITVINQKGEKKDVSGKTKKSRSGPDSTNIKL P47083 Something about silencing protein 10 Saccharomyces cerevisiae S288C 0.070364398 1 LEVO MVRKGSNRTKTSEVGDEINPYGLNEVDDFASKREKVLLGQSTFGDSNKDDDHSLLEDEDEEEVLAMDEDDESIDEREDEE EEEEEELDGAAAYKKIFGRNLETDQLPEEDEENGMLDNENAWGSTKGEYYGADDLDDDEAAKEIEKEALRQQKKHLEELN MNDYLDEEEEEEWVKSAKEFDMGEFKNSTKQADTKTSITDILNMDDEARDNYLRTMFPEFAPLSKEFTELAPKFDELKKS EENEFNKLKLIALGSYLGTISCYYSILLHELHNNEDFTSMKGHPVMEKILTTKEIWRQASELPSSFDVNEGDGSESEETA NIEAFNEKKLNELQNSEDSDAEDGGKQKQEIDEEERESDEEEEEEDVDIDDFEEYVAQSRLHSKPKTSSMPEADDFIESE IADVDAQDKKARRRTLRFYTSKIDQQENKKTDRFKGDDDIPYKERLFERQQRLLDEARKRGMHDNNGADLDDKDYGSEDE AVSRSINTQGENDYYQQVQRGKQDKKISRKEAHKNAVIAAREGKLAELAENVSGDGKRAINYQILKNKGLTPKRNKDNRN SRVKKRKKYQKAQKKLKSVRAVYSGGQSGVYEGEKTGIKKGLTRSVKFKN Q12136 40S ribosomal protein S13 Saccharomyces cerevisiae S288C 0.017059945 1 LEVO MGRMHSAGKGISSSAIPYSRNAPAWFKLSSESVIEQIVKYARKGLTPSQIGVLLRDAHGVTQARVITGNKIMRILKSNGL APEIPEDLYYLIKKAVSVRKHLERNRKDKDAKFRLILIESRIHRLARYYRTVAVLPPNWKYESATASALVN P05756 40S ribosomal protein S14-A Saccharomyces cerevisiae S288C 0.014562655 1 LEVO MSNVVQARDNSQVFGVARIYASFNDTFVHVTDLSGKETIARVTGGMKVKADRDESSPYAAMLAAQDVAAKCKEVGITAVH VKIRATGGTRTKTPGPGGQAALRALARSGLRIGRIEDVTPVPSDSTRKKGGRRGRRL P06367 Dimethyladenosine transferase Saccharomyces cerevisiae S288C 0.036003836000000004 1 LEVO MGKAAKKKYSGATSSKQVSAEKHLSSVFKFNTDLGQHILKNPLVAQGIVDKAQIRPSDVVLEVGPGTGNLTVRILEQAKN VVAVEMDPRMAAELTKRVRGTPVEKKLEIMLGDFMKTELPYFDICISNTPYQISSPLVFKLINQPRPPRVSILMFQREFA LRLLARPGDSLYCRLSANVQMWANVTHIMKVGKNNFRPPPQVESSVVRLEIKNPRPQVDYNEWDGLLRIVFVRKNRTISA GFKSTTVMDILEKNYKTFLAMNNEMVDDTKGSMHDVVKEKIDTVLKETDLGDKRAGKCDQNDFLRLLYAFHQVGIHFS P41819 2.1.1.183 Small ribosomal subunit protein eS1A Saccharomyces cerevisiae S288C 0.028798466999999998 1 LEVO MAVGKNKRLSKGKKGQKKRVVDPFTRKEWFDIKAPSTFENRNVGKTLVNKSTGLKSASDALKGRVVEVCLADLQGSEDHS FRKIKLRVDEVQGKNLLTNFHGMDFTTDKLRSMVRKWQTLIEANVTVKTSDDYVLRIFAIAFTRKQANQVKRHSYAQSSH IRAIRKVISEILTKEVQGSTLAQLTSKLIPEVINKEIENATKDIFPLQNIHVRKVKLLKQPKFDVGALMALHGEGSGEEK GKKVTGFKDEVLETV P33442 40S ribosomal protein S19-A Saccharomyces cerevisiae S288C 0.015942124999999998 1 LEVO MPGVSVRDVAAQDFINAYASFLQRQGKLEVPGYVDIVKTSSGNEMPPQDAEGWFYKRAASVARHIYMRKQVGVGKLNKLY GGAKSRGVRPYKHIDASGSINRKVLQALEKIGIVEISPKGGRRISENGQRDLDRIAAQTLEEDE P07280 40S ribosomal protein S27-A Saccharomyces cerevisiae S288C 0.008893390999999999 1 LEVO MVLVQDLLHPTAASEARKHKLKTLVQGPRSYFLDVKCPGCLNITTVFSHAQTAVTCESCSTILCTPTGGKAKLSEGTSFR RK P35997 Probable ATP-dependent RNA helicase DHR1 Saccharomyces cerevisiae S288C 0.14517140599999998 1 LEVO MGTYRKRFNEKARSGHMAKLKELKRIRNKQFTRQDENDERVENPDSAPAESSTTEPNANAEILEPLTEEEKKMKKRKLQE LFTPKESKVSRLKKKRLDKFIEHQLKREERKTIIGKLQDYKIDTSLLTSSKRLGEGRQTKKEEFKEALSLERQGRGNEQT NEILYEEYEPKVWDEYGEGGSSEDDDGEDDFEASFGSMPKPTDNEEKKSSGFIDHRPAKFGGSGLSFGFSNIKVINKESK TPKKKYNWRQRVEMEELKKHGKEDEMDFDTTSEDDDEEEDQEEEDKMHPSENPLEEVESADSETGSEKFDQNDVANEFKD WANQEIKKLEGRDQELVTPTLNIDYKPIIRKEDLDDGLQEAYVPINENSTRKAFYVEVSRSDEIQKARIQLPVFGEEHKI MEAIHHNDVVIICGETGSGKTTQVPQFLYEAGFGAEDSPDYPGMVGITQPRRVAAVSMAERVANELGDHGHKVGYQIRFD STAKEDTKVKFMTDGVLLREMMHDFKLTKYSSIIIDEAHERNINTDILIGMLSRCVRLRAKLHKENPIEHKKLKLIIMSA TLRVSDFSENKTLFPIAPPVLQVDARQFPVSIHFNRRTAFNYTDEAFRKTCKIHQKLPPGAILVFLTGQQEITHMVKRLR KEFPFKKNSKYNKDLETPVSKMGINSKTTDLEAEDIDFSVQVIDQDKFKSAIRYEEDEGNSGNGEDEEDEEEEGFEEVLT EGQTANDPLYVLPLYSLLPTKEQMRVFQKPPQGSRLCIVATNVAETSLTIPGVRYVVDSGRSKERKYNESNGVQSFEVGW VSKASANQRSGRAGRTGPGHCYRLYSSAVFEHDFEQFSKPEILRMPVESIVLQMKSMAIHNIINFPFPTPPDRVALSKAI QLLQYLGALDNKEMITEDGKKMSLFPLSPRFSKMLLVSDEKACLPYIVAIVSALSVGDPFINEFELGINEISRKPNPDEN LDDKIREHDESTPGMDPELKKELRSKFYKSRSQFSKLDKFSDVFRLLSVVSAMDYVPKEQKEIFMKKNFLRGKLMEEIVK LRKQLMYIIKSNTSKENIAVVIRNEDLKSDIPSVIQIKLLKQMICAGFVDHVAVRADVLFPDDAKITNRTSIINIPYIPV LATRTPNIEDCFVYIHPTSILNNLGEMPPKYMLYYSLHLGGNNKTRMNTLCDIASTPLANIARKGLLLTYSKPLTGQGLK TVNLSPTERYCYVVPRFGSTVDNDLKIGWDLNPIAVHQKKQKGQWTVIKFITRKGFQTITGEEKEKK Q04217 3.6.4.13 40S ribosomal protein S25-A Saccharomyces cerevisiae S288C 0.012067272 1 LEVO MPPKQQLSKAAKAAAALAGGKKSKKKWSKKSMKDRAQHAVILDQEKYDRILKEVPTYRYVSVSVLVDRLKIGGSLARIAL RHLEKEGIIKPISKHSKQAIYTRATASE Q3E792 Ribosomal RNA-processing protein 9 Saccharomyces cerevisiae S288C 0.065146969 1 LEVO MSDVTQQKKRKRSKGEVNPSKPTVDEEITDPSSNEDEQLEVSDEEDALESEEEFEGENPADKRRRLAKQYLENLKSEAND ILTDNRNAEEKDLNNLKERTIDEYNNFDAGDLDKDIIASRLKEDVAEQQGRVFRYFGDKLLISEAKQSFTRVGENNLTCI SCFQPVLNKYTFEESSNGDKNKGRLFAYTVSKDLQLTKYDITDFSKRPKKLKYAKGGAKYIPTSKHEYENTTEGHYDEIL TVAASPDGKYVVTGGRDRKLIVWSTESLSPVKVIPTKDRRGEVLSLAFRKNSDQLYASCADFKIRTYSINQFSQLEILYG HHDIVEDISALAMERCVTVGARDRTAMLWKIPDETRLTFRGGDEPQKLLRRWMKENAKEGEDGEVKYPDESEAPLFFCEG SIDVVSMVDDFHFITGSDNGNICLWSLAKKKPIFTERIAHGILPEPSFNDISGETDEELRKRQLQGKKLLQPFWITSLYA IPYSNVFISGSWSGSLKVWKISDNLRSFELLGELSGAKGVVTKIQVVESGKHGKEKFRILASIAKEHRLGRWIANVSGAR NGIYSAVIDQTGF Q06506 RNA 3'-terminal phosphate cyclase-like protein Saccharomyces cerevisiae S288C 0.040220558999999996 1 LEVO MSSSAPKYTTFQGSQNFRLRIVLATLSGKPIKIEKIRSGDLNPGLKDYEVSFLRLIESVTNGSVIEISYTGTTVIYRPGI IVGGASTHICPSSKPVGYFVEPMLYLAPFSKKKFSILFKGITASHNDAGIEAIKWGLMPVMEKFGVRECALHTLKRGSPP LGGGEVHLVVDSLIAQPITMHEIDRPIISSITGVAYSTRVSPSLVNRMIDGAKKVLKNLQCEVNITADVWRGENSGKSPG WGITLVAQSKQKGWSYFAEDIGDAGSIPEELGEKVACQLLEEISKSAAVGRNQLPLAIVYMVIGKEDIGRLRINKEQIDE RFIILLRDIKKIFNTEVFLKPVDEADNEDMIATIKGIGFTNTSKKIA Q08096 Ribosome biogenesis protein BMS1 Saccharomyces cerevisiae S288C 0.135792281 1 LEVO MEQSNKQHRKAKEKNTAKKKLHTQGHNAKAFAVAAPGKMARTMQRSSDVNERKLHVPMVDRTPEDDPPPFIVAVVGPPGT GKTTLIRSLVRRMTKSTLNDIQGPITVVSGKHRRLTFLECPADDLNAMIDIAKIADLVLLLIDGNFGFEMETMEFLNIAQ HHGMPRVLGVATHLDLFKSQSTLRASKKRLKHRFWTEVYQGAKLFYLSGVINGRYPDREILNLSRFISVMKFRPLKWRNE HPYMLADRFTDLTHPELIETQGLQIDRKVAIYGYLHGTPLPSAPGTRVHIAGVGDFSVAQIEKLPDPCPTPFYQQKLDDF EREKMKEEAKANGEITTASTTRRRKRLDDKDKLIYAPMSDVGGVLMDKDAVYIDIGKKNEEPSFVPGQERGEGEKLMTGL QSVEQSIAEKFDGVGLQLFSNGTELHEVADHEGMDVESGEESIEDDEGKSKGRTSLRKPRIYGKPVQEEDADIDNLPSDE EPYTNDDDVQDSEPRMVEIDFNNTGEQGAEKLALETDSEFEESEDEFSWERTAANKLKKTESKKRTWNIGKLIYMDNISP EECIRRWRGEDDDSKDESDIEEDVDDDFFRKKDGTVTKEGNKDHAVDLEKFVPYFDTFEKLAKKWKSVDAIKERFLGAGI LGNDNKTKSDSNEGGEELYGDFEDLEDGNPSEQAEDNSDKESEDEDENEDTNGDDDNSFTNFDAEEKKDLTMEQEREMNA AKKEKLRAQFEIEEGENFKEDDENNEYDTWYELQKAKISKQLEINNIEYQEMTPEQRQRIEGFKAGSYVRIVFEKVPMEF VKNFNPKFPIVMGGLLPTEIKFGIVKARLRRHRWHKKILKTNDPLVLSLGWRRFQTLPIYTTTDSRTRTRMLKYTPEHTY CNAAFYGPLCSPNTPFCGVQIVANSDTGNGFRIAATGIVEEIDVNIEIVKKLKLVGFPYKIFKNTAFIKDMFSSAMEVAR FEGAQIKTVSGIRGEIKRALSKPEGHYRAAFEDKILMSDIVILRSWYPVRVKKFYNPVTSLLLKEKTEWKGLRLTGQIRA AMNLETPSNPDSAYHKIERVERHFNGLKVPKAVQKELPFKSQIHQMKPQKKKTYMAKRAVVLGGDEKKARSFIQKVLTIS KAKDSKRKEQKASQRKERLKKLAKMEEEKSQRDKEKKKEYFAQNGKRTTMGGDDESRPRKMRR Q08965 Ribosomal RNA small subunit methyltransferase NEP1 Saccharomyces cerevisiae S288C 0.027936461 2 LEVO MVEDSRVRDALKGGDQKALPASLVPQAPPVLTSKDKITKRMIVVLAMASLETHKISSNGPGGDKYVLLNCDDHQGLLKKM GRDISEARPDITHQCLLTLLDSPINKAGKLQVYIQTSRGILIEVNPTVRIPRTFKRFSGLMVQLLHKLSIRSVNSEEKLL KVIKNPITDHLPTKCRKVTLSFDAPVIRVQDYIEKLDDDESICVFVGAMARGKDNFADEYVDEKVGLSNYPLSASVACSK FCHGAEDAWNIL Q06287 2.1.1.260 rRNA-processing protein FCF1 Saccharomyces cerevisiae S288C 0.021650728999999997 1 LEVO MGKAKKTRKFGLVKRTLNTKKDQRLKKNQENIKTKEDPELTRNIPQVSSALFFQYNQAIKPPYQVLIDTNFINFSIQKKV DIVRGMMDCLLAKCNPLITDCVMAELEKLGPKYRIALKLARDPRIKRLSCSHKGTYADDCLVHRVLQHKCYIVATNDAGL KQRIRKIPGIPLMSVGGHAYVIEKLPDVF Q05498 U3 small nucleolar ribonucleoprotein protein IMP4 Saccharomyces cerevisiae S288C 0.033536168 1 LEVO MLRRQARERREYLYRKAQELQDSQLQQKRQIIKQALAQGKPLPKELAEDESLQKDFRYDQSLKESEEADDLQVDDEYAAT SGIMDPRIIVTTSRDPSTRLSQFAKEIKLLFPNAVRLNRGNYVMPNLVDACKKSGTTDLVVLHEHRGVPTSLTISHFPHG PTAQFSLHNVVMRHDIINAGNQSEVNPHLIFDNFTTALGKRVVCILKHLFNAGPKKDSERVITFANRGDFISVRQHVYVR TREGVEIAEVGPRFEMRLFELRLGTLENKDADVEWQLRRFIRTANKKDYL P53941 U3 small nucleolar RNA-associated protein 20 Saccharomyces cerevisiae S288C 0.2879155 1 LEVO MAKQRQTTKSSKRYRYSSFKARIDDLKIEPARNLEKRVHDYVESSHFLASFDQWKEINLSAKFTEFAAEIEHDVQTLPQI LYHDKKIFNSLVSFINFHDEFSLQPLLDLLAQFCHDLGPDFLKFYEEAIKTLINLLDAAIEFESSNVFEWGFNCLAYIFK YLSKFLVKKLVLTCDLLIPLLSHSKEYLSRFSAEALSFLVRKCPVSNLREFVRSVFEKLEGDDEQTNLYEGLLILFTESM TSTQETLHSKAKAIMSVLLHEALTKSSPERSVSLLSDIWMNISKYASIESLLPVYEVMYQDFNDSLDATNIDRILKVLTT IVFSESGRKIPDWNKITILIERIMSQSENCASLSQDKVAFLFALFIRNSDVKTLTLFHQKLFNYALTNISDCFLEFFQFA LRLSYERVFSFNGLKFLQLFLKKNWQSQGKKIALFFLEVDDKPELQKVREVNFPEEFILSIRDFFVTAEINDSNDLFEIY WRAIIFKYSKLQNTEIIIPLLERIFSTFASPDNFTKDMVGTLLKIYRKEDDASGNNLLKTILDNYENYKESLNFLRGWNK LVSNLHPSESLKGLMSHYPSLLLSLTDNFMLPDGKIRYETLELMKTLMILQGMQVPDLLSSCMVIEEIPLTLQNARDLTI RIKNVGAEFGKTKTDKLVSSFFLKYLFGLLTVRFSPVWTGVFDTLPNVYTKDEALVWKLVLSFIKLPDENQNLDYYQPLL EDGANKVLWDSSVVRLRDTIDTFSHIWSKYSTQNTSIISTTIERRGNTTYPILIRNQALKVMLSIPQVAENHFVDIAPFV YNDFKTYKDEEDMENERVITGSWTEVDRNVFLKTLSKFKNIKNVYSATELHDHLMVLLGSRNTDVQKLALDALLAYKNPT LNKYRDNLKNLLDDTLFKDEITTFLTENGSQSIKAEDEKVVMPYVLRIFFGRAQVPPTSGQKRSRKIAVISVLPNFKKPY INDFLSLASERLDYNYFFGNSHQINSSKATLKTIRRMTGFVNIVNSTLSVLRTNFPLHTNSVLQPLIYSIAMAYYVLDTE STEEVHLRKMASNLRQQGLKCLSSVFEFVGNTFDWSTSMEDIYAVVVKPRISHFSDENLQQPSSLLRLFLYWAHNPSLYQ FLYYDEFATATALMDTISNQHVKEAVIGPIIEAADSIIRNPVNDDHYVDLVTLICTSCLKILPSLYVKLSDSNSISTFLN LLVSITEMGFIQDDHVRSRLISSLISILKGKLKKLQENDTQKILKILKLIVFNYNCSWSDIEELYTTISSLFKTFDERNL RVSLTELFIELGRKVPELESISKLVADLNSYSSSRMHEYDFPRILSTFKGLIEDGYKSYSELEWLPLLFTFLHFINNKEE LALRTNASHAIMKFIDFINEKPNLNEASKSISMLKDILLPNIRIGLRDSLEEVQSEYVSVLSYMVKNTKYFTDFEDMAIL LYNGDEEADFFTNVNHIQLHRRQRAIKRLGEHAHQLKDNSISHYLIPMIEHYVFSDDERYRNIGNETQIAIGGLAQHMSW NQYKALLRRYISMLKTKPNQMKQAVQLIVQLSVPLRETLRIVRDGAESKLTLSKFPSNLDEPSNFIKQELYPTLSKILGT RDDETIIERMPIAEALVNIVLGLTNDDITNFLPSILTNICQVLRSKSEELRDAVRVTLGKISIILGAEYLVFVIKELMAT LKRGSQIHVLSYTVHYILKSMHGVLKHSDLDTSSSMIVKIIMENIFGFAGEEKDSENYHTKVKEIKSNKSYDAGEILASN ISLTEFGTLLSPVKALLMVRINLRNQNKLSELLRRYLLGLNHNSDSESESILKFCHQLFQESEMSNSPQIPKKKVKDQVD EKEDFFLVNLESKSYTINSNSLLLNSTLQKFALDLLRNVITRHRSFLTVSHLEGFIPFLRDSLLSENEGVVISTLRILIT LIRLDFSDESSEIFKNCARKVLNIIKVSPSTSSELCQMGLKFLSAFIRHTDSTLKDTALSYVLGRVLPDLNEPSRQGLAF NFLKALVSKHIMLPELYDIADTTREIMVTNHSKEIRDVSRSVYYQFLMEYDQSKGRLEKQFKFMVDNLQYPTESGRQSVM ELINLIITKANPALLSKLSSSFFLALVNVSFNDDAPRCREMASVLISTMLPKLENKDLEIVEKYIAAWLKQVDNASFLNL GLRTYKVYLKSIGFEHTIELDELAIKRIRYILSDTSVGSEHQWDLVYSALNTFSSYMEATESVYKHGFKDIWDGIITCLL YPHSWVRQSAANLVHQLIANKDKLEISLTNLEIQTIATRILHQLGAPSIPENLANVSIKTLVNISILWKEQRTPFIMDVS KQTGEDLKYTTAIDYMVTRIGGIIRSDEHRMDSFMSKKACIQLLALLVQVLDEDEVIAEGEKILLPLYGYLETYYSRAVD EEQEELRTLSNECLKILEDKLQVSDFTKIYTAVKQTVLERRKERRSKRAILAVNAPQISADKKLRKHARSREKRKHEKDE NGYYQRRNKRKRA P35194 40S ribosomal protein S23-A Saccharomyces cerevisiae S288C 0.016073896 1 LEVO MGKGKPRGLNSARKLRVHRRNNRWAENNYKKRLLGTAFKSSPFGGSSHAKGIVLEKLGIESKQPNSAIRKCVRVQLIKNG KKVTAFVPNDGCLNFVDENDEVLLAGFGRKGKAKGDIPGVRFKVVKVSGVSLLALWKEKKEKPRS P0CX29 U3 small nucleolar RNA-associated protein 14 Saccharomyces cerevisiae S288C 0.10326972699999999 1 LEVO MAKKKSKSRSKSSRRVLDALQLAEREINGEFDNSSDNDKRHDARRNGTVVNLLKRSKGDTNSDEDDIDSESFEDEELNSD EALGSDDDYDILNSKFSQTIRDKKENANYQEEEDEGGYTSIDEEDLMPLSQVWDMDEKTAQSNGNDDEDASPQLKLQDTD ISSESSSSEESESESEDDEEEEDPFDEISEDEEDIELNTITSKLIDETKSKAPKRLDTYGSGEANEYVLPSANAASGASG KLSLTDMMNVIDDRQVIENANLLKGKSSTYEVPLPQRIQQRHDRKAAYEISRQEVSKWNDIVQQNRRADHLIFPLNKPTE HNHASAFTRTQDVPQTELQEKVDQVLQESNLANPEKDSKFEELSTAKMTPEEMRKRTTEMRLMRELMFREERKARRLKKI KSKTYRKIKKKELMKNRELAAV(SEP)SDEDNEDHDIARAKERMTLKHKTNSKWAKDMIKHGMTNDAETREEMEEMLRQG ERLKAKMLDRNSDDEEDGRVQTLSDVENEEKENIDSEALKSKLGKTGVMNMAFMKNGEAREREANKETLRQLRAVENGDD IKLFESDEEETNGENIQINKGRRIYTPGSLESNKDMNELNDHTRKENKVDESRSLENRLRAKNSGQSKNARTNAEGAIIV EEESDGEPLQDGQNNQQDEEAKDVNPWLANESDEEHTVKKQSSKVNVIDKDSSKNVKAMNKMEKAELKQKKKKKGKSNDD EDLLLTADDSTRLKIVDPYGGSDDEQGDNVFMFKQQDVIAEAFAGDDVVAEFQEEKKRVIDDEDDKEVDTTLPGWGEWAG AGSKPKNKKRKFIKKVKGVVNKDKRRDKNLQNVIINEKVNKKNLKYQSSAVPFPFENREQYERSLRMPIGQEWTSRASHQ ELIKPRIMTKPGQVIDPLKAPFK Q04500 Nucleolar complex protein 14 Saccharomyces cerevisiae S288C 0.094463195 1 LEVO MAGSQLKNLKAALKARGLTGQTNVKSKNKKNSKRQAKEYDREEKKKAIAEIREEFNPFEIKAARNKRRDGLPSKTADRIA VGKPGISKQIGEEQRKRAFEARKMMKNKRGGVIDKRFGERDKLLTEEEKMLERFTRERQSQSKRNANLFNLEDDEDDGDM FGDGLTHLGQSLSLEDELANDEEDFLASKRFNEDDAELQQPQRKKTKAEVMKEVIAKSKFYKQERQKAQGIMEDQIDNLD DNFEDVMSELMMTQPKKNPMEPKTDLDKEYDIKVKELQLDKRAAPSDRTKTEEEKNAEAEEKKRELEQQRLDRMNGMIEL EEGEERGVEDLDDGFWENEEDYEDDNDGIADSDDDIKFEDQGRDEGFSQILKKKNISISCPRTHDALLDQVKKLDLDDHP KIVKNIIKAYQPKLAEGNKEKLGKFTAVLLRHIIFLSNQNYLKNVQSFKRTQNALISILKSLSEKYNRELSEECRDYINE MQARYKKNHFDALSNGDLVFFSIIGILFSTSDQYHLVITPALILMSQFLEQIKFNSLKRIAFGAVLVRIVSQYQRISKRY IPEVVYFFQKILLTFIVEKENQEKPLDFENIRLDSYELGLPLDVDFTKKRSTIIPLHTLSTMDTEAHPVDQCVSVLLNVM ESLDATISTVWKSLPAFNEIILPIQQLLSAYTSKYSDFEKPRNILNKVEKLTKFTEHIPLALQNHKPVSIPTHAPKYEEN FNPDKKSYDPDRTRSEINKMKAQLKKERKFTMKEIRKDAKFEARQRIEEKNKESSDYHAKMAHIVNTINTEEGAEKNKYE RERKLRGGKK Q99207 Nucleolar complex protein 4 Saccharomyces cerevisiae S288C 0.063707844 1 LEVO MVLLISEIKDIAKRLTAAGDRKQYNSIIKLINELVIPENVTQLEEDETEKNLRFLVMSLFQIFRKLFSRGDLTLPSSKKS TLEKEQFVNWCRKVYEAFKTKLLAIISDIPFETSLGLDSLDVYLQLAELESTHFASEKGAPFFPNKTFRKLIIALWSSNM GEIEDVKSSGASENLIIVEFTEKYYTKFADIQYYFQSEFNQLLEDPAYQDLLLKNVGKWLALVNHDKHCSSVDADLEIFV PNPPQAIENESKFKSNFEKNWLSLLNGQLSLQQYKSILLILHKRIIPHFHTPTKLMDFLTDSYNLQSSNKNAGVVPILAL NGLFELMKRFNLEYPNFYMKLYQIINPDLMHVKYRARFFRLMDVFLSSTHLSAHLVASFIKKLARLTLESPPSAIVTVIP FIYNLIRKHPNCMIMLHNPAFISNPFQTPDQVANLKTLKENYVDPFDVHESDPELTHALDSSLWELASLMEHYHPNVATL AKIFAQPFKKLSYNMEDFLDWNYDSLLNAESSRKLKTLPTLEFEAFTNVFDNEDGDSEASSQGNVYLPGVAW Q06512 Pre-rRNA-processing protein PNO1 Saccharomyces cerevisiae S288C 0.030380623 1 LEVO MVAPTALKKATVTPVSGQDGGSSRIIGINNTESIDEDDDDDVLLDDSDNNTAKEEVEGEEGSRKTHESKTVVVDDQGKPR FTSASKTQGNKIKFESRKIMVPPHRMTPLRNSWTKIYPPLVEHLKLQVRMNLKTKSVELRTNPKFTTDPGALQKGADFIK AFTLGFDLDDSIALLRLDDLYIETFEVKDVKTLTGDHLSRAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHI RMARESVVSLILGSPPGKVYGNLRTVASRLKERY Q99216 Essential nuclear protein 1 Saccharomyces cerevisiae S288C 0.055207422 1 LEVO MARASSTKARKQRHDPLLKDLDAAQGTLKKINKKKLAQNDAANHDAANEEDGYIDSKASRKILQLAKEQQDEIEGEELAE SERNKQFEARFTTMSYDDEDEDEDEDEEAFGEDISDFEPEGDYKEEEEIVEIDEEDAAMFEQYFKKSDDFNSLSGSYNLA DKIMASIREKESQVEDMQDDEPLANEQNTSRGNISSGLKSGEGVALPEKVIKAYTTVGSILKTWTHGKLPKLFKVIPSLR NWQDVIYVTNPEEWSPHVVYEATKLFVSNLTAKESQKFINLILLERFRDNIETSEDHSLNYHIYRAVKKSLYKPSAFFKG FLFPLVETGCNVREATIAGSVLAKVSVPALHSSAALSYLLRLPFSPPTTVFIKILLDKKYALPYQTVDDCVYYFMRFRIL DDGSNGEDATRVLPVIWHKAFLTFAQRYKNDITQDQRDFLLETVRQRGHKDIGPEIRRELLAGASREFVDPQEANDDLMI DVN P38333 MAGNESIUM ION 2.4305e-05 47 MG ZINC ION 6.5409e-05 2 ZN GUANOSINE-5'-TRIPHOSPHATE 0.0005231799999999999 1 GTP singleParticle particle 7.5 60.0 Tris-HCl 50.0 NaCl 5.0 MgCl 2.0 glycerol 0.01 NP-40 1.0 DTT ETHANE TFS KRIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 1.0 25.0 GATAN K3 (6k x 4k) 61.6 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 3.84 FSC 0.143 CUT-OFF 14551 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_49090.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 504 504 504 0 0 0 504 504 504 535.75195 535.75195 535.75195 90.0 90.0 90.0 X Y Z -0.4718449 5.0158134 0.08428682 0.14815165 1.063 1.063 1.063 0.85 AUTHOR