2026-01-21 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-02-10 2026-01-21 2026-01-21 2026-01-21 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35GM118093 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R01GM029832 United States CryoEM structure of U7 Sm Ring in complex with symplekin N-terminal domain Desotell A Tong L Symplekin, 3' end processing, U7 snRNP, Histone pre-mRNA, RNA BINDING PROTEIN, RNA BINDING PROTEIN-RNA complex Desotell A Marzluff WF Dominski Z Tong L An N-terminal helix of Lsm11 stabilizes CPSF73 in U7 snRNP for histone pre-mRNA 3'-end processing. Nucleic Acids Res. UK 54 2026 41495886 doi:10.1093/nar/gkaf1442 1362-4962 0389 NARHAD 9n96 FULLOVERLAP EMDB EMD-49161 associated EM volume
CryoEM stucture of U7 Sm Ring in complex with symplekin N-terminal domain
 U7 Sm Ring in complex with symplekin N-terminal domain U7 Sm Ring in complex with symplekin N-terminal domain 0 1 2 3 4 5 6 7 8 9 10 Homo sapiens Small nuclear ribonucleoprotein Sm D3 Homo sapiens 0.016111671 1 Escherichia coli BL21(DE3) LEVO MGSSHHHHHHSSGLVPRGSHMSIGVPIKVLHEAEGHIVTCETNTGEVYRGKLIEAEDNMNCQMSNITVTYRDGRVAQLEQ VYIRGSKIRFLILPDMLKNAPMLKSMKNKNQGSGAGRGKAAILKAQVAARGRGRGMGRGNIFQKRR P62318 Small nuclear ribonucleoprotein-associated proteins B and B' Homo sapiens 0.010911931 1 Escherichia coli BL21(DE3) LEVO MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAEREEKRVLGLVLLRGENLVSM TVEGPPPKDTGIARV P14678 U7 snRNA-associated Sm-like protein LSm10 Homo sapiens 0.014102057 1 Trichoplusia ni LEVO MAVSHSVKERTISENSLIILLQGLQGRVTTVDLRDESVAHGRIDNVDAFMNIRLAKVTYTDRWGHQVKLDDLFVTGRNVR YVHIPDDVNITSTIEQQLQIIHRVRNFGGKGQGRWEFPPKNCK Q969L4 U7 snRNA-associated Sm-like protein LSm11 Homo sapiens 0.028399312 1 Trichoplusia ni LEVO MHHHHHHSGGSMEERERGARSAGAGSPARPPSPRLDVSSDSFDPLLALYAPRLPPIPYPNAPCFNNVAEYESFLRTGVRG GGRGRGRARGAAAGSGVPAAPGPSGRTRRRPDAPAPDPEEAQEAERAMVAKEEGDGAAGAGRRGPGRSRKAPRNVLTRMP LHEGSPLGELHRCIREGVKVNVHIRTFKGLRGVCTGFLVAFDKFWNMALTDVDETYRKPVLQSRKKKRKPKVDYQQVFTR HINQIFIRGENVLLVHLAQ Small nuclear ribonucleoprotein E Homo sapiens 0.010817601 1 Escherichia coli BL21(DE3) LEVO MAYRGQGQKVQKVMVQPINLIFRYLQNRSRIQVWLYEQVNMRIEGCIIGFDEYMNLVLDDAEEIHSKTKSRKQLGRIMLK GDNITLLQSVSN P62304 Small nuclear ribonucleoprotein F Homo sapiens 0.009734171 1 Escherichia coli BL21(DE3) LEVO MSLPLNPKPFLNGLTGKPVMVKLKWGMEYKGYLVSVDGYMNMQLANTEEYIDGALSGHLGEVLIRCNNVLYIRGVEEEEE DGEMRE P62306 Small nuclear ribonucleoprotein G Homo sapiens 0.009579236 1 Escherichia coli BL21(DE3) LEVO MSKAHPPELKKFMDKKLSLKLNGGRHVQGILRGFDPFMNLVIDECVEMATSGQQNNIGMVVIRGNSIIMLEALERVLEHH HHHH P62308 Symplekin Homo sapiens 0.120355125 1 Trichoplusia ni LEVO MTTSERVVDLLNQAALITNDSKITVLKQVQELIINKDPTLLDNFLDEIIAFQADKSIEVRKFVIGFIEEACKRDIELLLK LIANLNMLLRDENVNVVKKAILTMTQLYKVALQWMVKSRVISELQEACWDMVSAMAGDIILLLDSDNDGIRTHAIKFVEG LIVTLSPRMADSEIPRRQEHDISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTTALGSLANIARQRPM FMSEVIQAYETLHANLPPTLAKSQVSSVRKNLKLHLLSVLKHPASLEFQAQITTLLVDLGTPQAEIARNMPSSKDTRKRP RDDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQISGQSDTDITAEFLQPLLTPDNVANLVLISMVYLPEAMPAS FQAIYTPVESAGTEAQIKHLARLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKTESVLIKRRLSAQGQAISVVGSLSSM SPLEEEAPQAKRRPEPIIPVTQPRLAGAGGRKKIFRLSDVLKPLTDAQVEAMKLGAVKRILRAEKAVACSGAAQVRIKIL ASLVTQFNSGLKAEVLSFILEDVRARLDLAFAWLYQEYNAYLAAGASGSLDKYEDCLIRLLSGLQEKPDQKDGIFTKVVL EAPLITESALEVVRKYCEDESRTYLGMSTLRDLIFKRPSRQFQYLHVLLDLSSHEKDKVRSQALLFIKRMYEKEQLREYV EKFALNYLQLLVHPNPPSVLFGADKDTEVAAPWTEETVKQCLYLYLALLPQNHKLIHELAAVYTEAIADIKRTVLRVIEQ PIRGMGMNSPELLLLVENCPKGAETLVTRCLHSLTDKVPPSPELVKRVRDLYHKRLPDVRFLIPVLNGLEKKEVIQALPK LIKLNPIVVKEVFNRLLGTQHGEGNSALSPLNPGELLIALHNIDSVKCDMKSIIKATNLCFAERNVYTSEVLAVVMQQLM EQSPLPMLLMRTVIQSLTMYPRLGGFVMNILSRLIMKQVWKYPKVWEGFIKCCQRTKPQSFQVILQLPPQQLGAVFDKCP ELREPLLAHVRSFTPHQQAHIPNSIMTILEAS Q92797 modified H2a pre-mRNA Mus musculus 0.019237537 1 CCAAAGGCUCUUUUCAGAGCCACCCACUGAAUCAGAUAAAGAGCUGUAACACGGUAGCCA U7 snRNA Homo sapiens 0.020013708999999998 1 CAGUGUUACAGCUCUUUUAGAAUUUGUCUAGUAGGCUUUCUGGCUUUUUACCGGAAAGCCCCU U47924.1 singleParticle particle 0.5 7.5 20.0 HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid 100.0 NaCl sodium chloride 10.0 EDTA Ethylenediaminetetraacetic Acid 5.0 DTT Dithiothreitol UltrAuFoil R1.2/1.3 GOLD 300 GLOW DISCHARGE OTHER ETHANE 100 295 FEI VITROBOT MARK IV TFS KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 1.0 2.5 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K3 (6k x 4k) 56.1 1 8741039 PHASE FLIPPING AND AMPLITUDE CORRECTION 6V4X 3.18 FSC 0.143 CUT-OFF 236295 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_49161.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 324.9 324.9 324.9 90.0 90.0 90.0 X Y Z -1.4675004 2.2868793 0.00009692904 0.030072762 1.083 1.083 1.083 0.25 AUTHOR CryoEM structure of U7 Sm Ring in complex with symplekin N-terminal domain emd_49161_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 324.9 324.9 324.9 90.0 90.0 90.0 X Y Z -0.4564336 0.88473296 0.00037969134 0.018780628 1.083 1.083 1.083 AUTHOR Half Map A emd_49161_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 324.9 324.9 324.9 90.0 90.0 90.0 X Y Z -0.4345426 0.887887 0.00037985516 0.018798435 1.083 1.083 1.083 AUTHOR Half Map B