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    <admin>
        <current_status>
            <date>2026-02-25</date>
            <code>REL</code>
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        </current_status>
        <revision_history>
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                        <revision_type>INITIAL_RELEASE</revision_type>
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                        <provider>REPOSITORY</provider>
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        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-02-11</deposition>
            <header_release>2026-02-25</header_release>
            <map_release>2026-02-25</map_release>
            <update>2026-02-25</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)</funding_body>
                <code>75N39019C00051</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Negative stain EM map of H1 HA (A/California/4/2009) in complex with monoclonal fab ST18</title>
        <authors_list>
            <author ORCID="0009-0009-6510-7558">Rodriguez AJ</author>
            <author ORCID="0000-0002-3402-2609">Han J</author>
            <author ORCID="0000-0001-7153-3769">Ward AB</author>
        </authors_list>
        <keywords>influenza, hemagglutinin, H1, monoclonal, complex, fab complex, viral fusion protein, VIRAL PROTEIN, VIRAL PROTEIN-IMMUNE SYSTEM complex</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author order="1">Fisher A</author>
                    <author order="2">Corcoran M</author>
                    <author order="3">Chernyshev M</author>
                    <author ORCID="0000-0002-2902-7739" order="4">Brouwer PJM</author>
                    <author order="5">Gillespie R</author>
                    <author order="6">Nicoletto A</author>
                    <author ORCID="0000-0002-5724-655X" order="7">Loeffler JR</author>
                    <author ORCID="0000-0003-2891-0850" order="8">Ferguson JA</author>
                    <author ORCID="0009-0009-6510-7558" order="9">Rodriguez AJ</author>
                    <author order="10">Narang S</author>
                    <author order="11">Adori M</author>
                    <author order="12">Angeletti D</author>
                    <author order="13">van Gils MJ</author>
                    <author order="14">Castro Dopico X</author>
                    <author order="15">Kanekiyo M</author>
                    <author ORCID="0000-0001-7153-3769" order="16">Ward AB</author>
                    <author ORCID="0000-0002-3402-2609" order="17">Han J</author>
                    <author order="18">Karlsson Hedestam GB</author>
                    <title>Individualized IG genotyping coupled with high throughput isolation of influenza HA-specific monoclonal antibodies</title>
                    <journal_abbreviation>To Be Published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-49192</accession_id>
                <content_type>associated EM volume</content_type>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>H1 HA (A/California/4/2009) in complex with monoclonal fab ST18</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>H1 HA (A/California/4/2009) in complex with monoclonal fab ST18</name>
                <parent>0</parent>
                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_source>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="2">
                <name>H1 HA (A/California/4/2009)</name>
                <parent>1</parent>
                <details>E47K stabilizing mutation in HA2</details>
                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_source>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="3">
                <name>ST18 monoclonal fab</name>
                <parent>1</parent>
                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
    </sample>
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            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
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                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">0.015</concentration>
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                        <ph>7.4</ph>
                    </buffer>
                    <staining>
                        <type>NEGATIVE</type>
                        <material>2% w/v Uranyl Formate</material>
                    </staining>
                    <grid>
                        <model>Homemade</model>
                        <material>COPPER</material>
                        <mesh>400</mesh>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                        </support_film>
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                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS TALOS F200C</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">200</acceleration_voltage>
                    <nominal_defocus_min units="µm">0.5</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.0</nominal_defocus_max>
                    <specimen_holder_model>SIDE ENTRY, EUCENTRIC</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>FEI CETA (4k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">25.0</average_electron_dose_per_image>
                        </image_recording>
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                    <type>NONE</type>
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                    <pdb_model>
                        <pdb_id>4M4Y</pdb_id>
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                    <resolution units="Å" res_type="BY AUTHOR">20.0</resolution>
                    <resolution_method>FSC 0.5 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>4.0b1</version>
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                    </software_list>
                    <number_images_used>1758</number_images_used>
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                    <type>MAXIMUM LIKELIHOOD</type>
                </initial_angle_assignment>
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                    <type>MAXIMUM LIKELIHOOD</type>
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        <label>::::EMDATABANK.org::::EMD-49192::::</label>
        <annotation_details>Negative stain EM map of H1 HA (A/California/4/2009) in complex with monoclonal fab ST18</annotation_details>
    </map>
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                    <gamma units="deg">90.0</gamma>
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                    <medium>Y</medium>
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                <label>::::EMDATABANK.org::::EMD-49192::::</label>
                <annotation_details>Negative stain EM half map A of H1 HA (A/California/4/2009) in complex with monoclonal fab ST18</annotation_details>
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                    <medium>Y</medium>
                    <slow>Z</slow>
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                    <std>0.004063912</std>
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                    <x units="Å">2.0</x>
                    <y units="Å">2.0</y>
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                <label>::::EMDATABANK.org::::EMD-49192::::</label>
                <annotation_details>Negative stain EM half map B of H1 HA (A/California/4/2009) in complex with monoclonal fab ST18</annotation_details>
            </half_map>
        </half_map_list>
    </interpretation>
</emd>
