2026-02-25 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-02-18 2026-02-25 2026-02-25 2026-02-25 Department of Energy (DOE, United States) DE-AC02-05CH11231 United States National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) R01HL115153 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R01GM104427 United States National Institutes of Health/National Institute of Mental Health (NIH/NIMH) R01MH077303 United States National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK) R01DK042667 United States A 3D density map of a 2D lattice formed by octahedral DNA origami with 70% loaded ferritin, was revealed by IMOD (Tomo #3). Liu J Ren G Non-averaged structure, individual particle cryo-electron tomography, IPET, cryo-ET, structural flexibility, DNA origami, 2D lattice, DNA-protein lattice, DNA Liu J Zhang M Wang S Hu Z Wu H Gang O Ren G Non-averaged 3D structure of low-ordered 2D lattice revealed by individual-particle cryo-electron tomography To Be Published 0353 EMDB EMD-49288 associated EM volume 2D lattice of octahedral DNA origami with 70% loaded ferritin 2D lattice of octahedral DNA origami with 70% loaded ferritin 0
The octahedral DNA origami was folded by mixing 20 nM of M13mp18 scaffold DNA with 100 nM of each staple oligonucleotide in TAE buffer containing 12.5 mM MgCl2. The mixed solution was then slowly cooled down and purified. Subsequently, the octahedral DNA origami was combined with ferritin in TAE/MgCl2 buffer, and the mixed solution was slowly cooled to room temperature.
synthetic construct tomography twoDArray 8.6 40.0 Tris 20.0 Acetate 1.0 EDTA 12.5 MgCl2 Magnesium dichloride ETHANE 90 277 LEICA EM GP
The octahedral DNA origami was folded by mixing 20 nM of M13mp18 scaffold DNA and 100 nM of each staple oligonucleotide in TAE buffer containing 12.5 mM MgCl2. The mixed solution was slowly cooled down and purified. The octahedral DNA origami was mixed with ferritin in TAE/MgCl2 buffer, then the mixed solution was slowly cooled to room temperature.
NO SECTIONING ZEISS LIBRA120PLUS FLOOD BEAM BRIGHT FIELD LAB6 120 2.2 7.0 7.0 80000.0 GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER NITROGEN 79.0 80.0 GATAN ULTRASCAN 4000 (4k x 4k) 1 33 1.0 2.0 1
The tilt series of whole micrographs were initially aligned with IMOD.
IPET 33 Gctf TOMOCTF
The Contrast Transfer Function (CTF) was determined by Gctf and then corrected by TOMOCTF.
PHASE FLIPPING AND AMPLITUDE CORRECTION emd_49288.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 1024 1024 250 -512 -512 -125 1024 1024 250 6062.08 6062.08 1480.0 90.0 90.0 90.0 X Y Z -331.947240000000022 350.984800000000007 0.000000003730435 50.041415999999998 5.92 5.92 5.92 AUTHOR