2026-03-11 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-02-19 2026-03-11 2026-03-11 2026-03-11 Other government 75N93019C00051 United States Polyclonal immune complex of Fab from serum of animal 6 at week 35 binding H1 HA Han J Leon AN Richey ST Ferguson JA Ward AB influenza virus, hemagglutinin, polyclonal antibody, VIRAL PROTEIN Akhtar A Styles TM Gu C Leon AN Tharp GK Stokdyk K Abbad A Hirst C Strohmeier S Neumann G Richey ST Ferguson JA Ahmed H Han J Carreno JM Davis CW Antia R Ward AB Kawaoka Y Krammer F Ahmed R Amara RR AS03-adjuvanted chimeric HA-based influenza vaccine induces long-lived stalk-specific plasma cells in bone marrow and lymph nodes of non-human primates To Be Published 0353 EMDB EMD-49324 associated EM volume Polyclonal immune complex of Fab from serum of animal 6 at week 35 binding H1 HA Polyclonal immune complex of Fab from serum of animal 6 at week 35 binding H1 HA 0 Macaca mulatta 0.28 Fab 1 Macaca mulatta hemagglutinin 1 Influenza A virus A/California/04/2009 E47K singleParticle particle 0.015 7.4 NEGATIVE 2% w/v uranyl formate Homemade COPPER 400 CARBON GLOW DISCHARGE FEI TECNAI 20 FLOOD BEAM DARK FIELD FIELD EMISSION GUN 120 70.0 2.7 1.0 1.5 73000.0 NITROGEN TVIPS TEMCAM-F416 (4k x 4k) 25.0 1 NONE 4M4Y
30A map generated in Chimera and used as starting model
 30.0 FSC 0.5 CUT-OFF RELION 3.0 70000 MAXIMUM LIKELIHOOD RELION MAXIMUM LIKELIHOOD RELION emd_49324.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 208 208 208 0 0 0 208 208 208 347.36 347.36 347.36 90.0 90.0 90.0 X Y Z -0.018001214 0.078486815 0.0001433675 0.002993122 1.67 1.67 1.67 0.0193 AUTHOR Negative stain map of global refinement of particle stack used to sort polyclonal responses to H1 HA (A/California/04/2009 H1 (HA2 E47K)) from animal 6 week 35 emd_49324_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 208 208 208 0 0 0 208 208 208 347.36 347.36 347.36 90.0 90.0 90.0 X Y Z -0.026899474 0.056340404 0.00012886988 0.0031904744 1.67 1.67 1.67 AUTHOR Negative stain map of polyclonal Fab binding the H1 HA central stem and angled stem epitopes from animal 6 week 35 emd_49324_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 208 208 208 0 0 0 208 208 208 347.36 347.36 347.36 90.0 90.0 90.0 X Y Z -0.021896105 0.05837284 0.00013880093 0.0031518587 1.67 1.67 1.67 AUTHOR Negative stain map of polyclonal Fab binding the H1 HA low stem epitope from animal 6 week 35 emd_49324_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 208 208 208 0 0 0 208 208 208 347.36 347.36 347.36 90.0 90.0 90.0 X Y Z -0.019250326 0.08257919 0.00014529012 0.0030465475 1.67 1.67 1.67 AUTHOR Negative stain half map A of global refinement of particle stack used to sort polyclonal responses to H1 HA (A/California/04/2009 H1 (HA2 E47K)) from animal 6 week 35 emd_49324_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 208 208 208 0 0 0 208 208 208 347.36 347.36 347.36 90.0 90.0 90.0 X Y Z -0.017726671 0.07870207 0.00014386089 0.0030446567 1.67 1.67 1.67 AUTHOR Negative stain half map B of global refinement of particle stack used to sort polyclonal responses to H1 HA (A/California/04/2009 H1 (HA2 E47K)) from animal 6 week 35