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    <admin>
        <current_status>
            <date>2026-03-11</date>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
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        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-03-05</deposition>
            <header_release>2026-03-11</header_release>
            <map_release>2026-03-11</map_release>
            <update>2026-03-11</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</funding_body>
                <code>R01GM024485</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Cryo-EM structure of Serendipita indica sulfate transporter SiSulT in oxlate bound state</title>
        <authors_list>
            <author>Khandelwal NK</author>
            <author>Kumar P</author>
            <author>Gupta M</author>
            <author>Finer-Moore J</author>
            <author>Stroud RM</author>
        </authors_list>
        <keywords>Transporter, MEMBRANE PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author ORCID="0000-0002-6319-8048" order="1">Khandelwal NK</author>
                    <author ORCID="0009-0007-1482-0009" order="2">Kumar P</author>
                    <author ORCID="0000-0002-7432-3211" order="3">Gupta M</author>
                    <author ORCID="0000-0001-8172-0998" order="4">Finer-Moore J</author>
                    <author ORCID="0000-0003-2083-5665" order="5">Stroud RM</author>
                    <title>Cryo-EM structure of Serendipita indica sulfate transporter SiSulT in oxlate bound state</title>
                    <journal_abbreviation>To Be Published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>9nnc</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-49571</accession_id>
                <content_type>associated EM volume</content_type>
                <details>Cryo-EM structure of Serendipita indica sulfate transporter SiSulT in oxlate bound state</details>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>Sulphate transporter with oxlate</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Sulphate transporter with oxlate</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="65672">Serendipita indica</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.20665154</theoretical>
                </molecular_weight>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Probable Sulfate permease</name>
                <natural_source database="NCBI">
                    <organism ncbi="65672">Serendipita indica</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.088222195</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="4932">Saccharomyces cerevisiae</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MASDYKDDDDKGALEVLFQGPSSPGAATVKRLAKKIVSAPEEAPPTVSSGDWIKSQLEGETPKTAVVHYLKSLFPVIQWA
PNYNIGWLYGDVVAGLTVGLVLIPQSMSYARLATLPTEYGLYASFVGVFIYCFFATSKDVSIGPVAVMSLEVANIIKYVQ
SHYGDRWGNVQIAVTLSFICGFIVLGIGLLRIGWIVEFIPTPAVAGFMTGSAITIVSSQVPGLFGIQNLLDTRTSAYKVI
INTLKNLGHSKKDAAFGVTGLFALYFIRWIFDYLGRRYPNRARTFFYLSVMRNAFVLIILTLAAWGVVRYEKPDKKGNYS
ISILKTVPRGFKHIGQPTIDPELLKGLGSHLFVATLILLLEHIAISKSFGRINGYKINPNQELIAIGVTNTIGTLFAAYP
ATGSFSRSALKSKCGVRTPAAGWVTGLVVIVALYGLTDAFFFIPTAGLSAIIVHAVADLVTPPSQVYRFWLISPLEFLIW
AAAVLVSIFSSIENGIYTSVAASLVLLLIRVARPGGQFLGKVKVHGDDNSTSRDVFVPLEPKGGLRNPHIIVEPAAPGVF
IFRLEESFTFPNSSLINSTVVDHIKEHTRRGKDVSLIRLIDRPWNDPGPRRGAAFDPKAQDTSKPLLKAVVLDFAAVGNI
DTTGVQNLIDTRKELENWADGPVEFHFANILSPWVRRGLVAGGFGTGKDVDFPAEVAPVVPNQSGDYADPDHQTPHLRHR
EEWISKTGTTKTDKTEADVESGDSEYRKSNSPSSSVEGPLLSTLTPFFHVDLASAVRVAEARAKRSTPGLVPRGSSAHHH
HHHHHHHGA</string>
                    <external_references type="UNIPROTKB">G4T6Y6</external_references>
                </sequence>
            </protein_or_peptide>
            <ligand macromolecule_id="2">
                <name>OXALATE ION</name>
                <molecular_weight>
                    <theoretical units="MDa">8.8019e-05</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <formula>OXL</formula>
            </ligand>
            <ligand macromolecule_id="3">
                <name>water</name>
                <molecular_weight>
                    <theoretical units="MDa">1.8015e-05</theoretical>
                </molecular_weight>
                <number_of_copies>4</number_of_copies>
                <formula>HOH</formula>
            </ligand>
        </macromolecule_list>
    </sample>
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        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">.9</concentration>
                    <buffer>
                        <ph>7.5</ph>
                        <component>
                            <concentration units="mM">300.0</concentration>
                            <formula>NaCl</formula>
                            <name>sodium chloride</name>
                        </component>
                        <component>
                            <concentration units="mM">50.0</concentration>
                            <formula>Tris-Cl</formula>
                            <name>Tris chloride</name>
                        </component>
                        <details>50 mM Tirs ,300mM  Nacl pH7.5- LMNG0.01%+.001%CHS</details>
                    </buffer>
                    <grid>
                        <model>Quantifoil R1.2/1.3</model>
                        <material>GOLD</material>
                        <mesh>400</mesh>
                        <pretreatment>
                            <type>GLOW DISCHARGE</type>
                            <time units="s">30</time>
                            <pressure units="kPa">330.0</pressure>
                        </pretreatment>
                        <details>30 glow 30 hold at 15mA</details>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">100</chamber_humidity>
                        <chamber_temperature units="K">281.81</chamber_temperature>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                    </vitrification>
                    <details>Sult-0.9mg /ml with +100mM Potassium oxalate monohydrate</details>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>OTHER</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">70.0</c2_aperture_diameter>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_defocus_min units="µm">1.0</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.5</nominal_defocus_max>
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                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 (6k x 4k)</film_or_detector_model>
                            <number_grids_imaged>1</number_grids_imaged>
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                            <average_exposure_time units="s">0.8189</average_exposure_time>
                            <average_electron_dose_per_image units="e/Å^2">47.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <particle_selection>
                    <number_selected>5967145</number_selected>
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                <ctf_correction>
                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                            <version>v4.2.1</version>
                        </software>
                    </software_list>
                    <type>NONE</type>
                </ctf_correction>
                <startup_model type_of_model="INSILICO MODEL">
                    <insilico_model>Ab initio model</insilico_model>
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                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>C2</point_group>
                    </applied_symmetry>
                    <resolution units="Å" res_type="BY AUTHOR">2.4</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
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                        <software>
                            <name>cryoSPARC</name>
                            <version>v4.2.1</version>
                        </software>
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                    <number_images_used>376034</number_images_used>
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                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
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                        <software>
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    </structure_determination_list>
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