2025-11-19 REL RCSB RCSB RCSB 2025-03-05 2025-11-19 2025-11-19 2025-11-19 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35GM152032 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) DP2GM150023 United States Consensus map of HSV1 helicase-primase in complex with a forked DNA He Q Baranovskiy AG Morstadt LM Babayeva ND Lim C Tahirov TH DNA replication, HSV-1 helicase-primase, VIRAL PROTEIN Baranovskiy AG He Q Suwa Y Morstadt LM Babayeva ND Lim CJ Tahirov TH Structural basis of herpesvirus helicase-primase inhibition by pritelivir and amenamevir. Sci Adv US 11 eadz1989 eadz1989 2025 41202142 doi:10.1126/sciadv.adz1989 2375-2548 EMDB EMD-49582 associated EM volume HSV-1 helicase-primase in complex with a forked DNA HSV-1 helicase-primase in complex with a forked DNA 0 1 2 3 4
HSV-1 helicase-primase in complex with a forked DNA sovled using cryo-EM single-particle analysis
Human alphaherpesvirus 1 strain 17 0.3 singleParticle particle 4.5 7.5 20.0 HEPES 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid 100.0 KCl potassium chloride 0.5 Glycerol Glycerol 2.0 DTT dithiothreitol 4.0 CHAPSO CHAPSO
CHAPSO is made fresh at 80 mM before being added to the sample at a final concentration of 4 mM immediately before vitrification
 ETHANE 95 277.15 FEI VITROBOT MARK IV
The sample was monodisperse
 TFS KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 1.0 2.5 81000.0 GATAN K3 (6k x 4k) 1 12555 50.0 1 4446980 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION From Cryosparc 1 C1 3.43 FSC 0.143 CUT-OFF cryoSPARC 431843 MAXIMUM LIKELIHOOD cryoSPARC MAXIMUM LIKELIHOOD emd_49582.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 385.2 385.2 385.2 90.0 90.0 90.0 X Y Z -0.10285953 0.2875637 0.00033502158 0.005704049 1.07 1.07 1.07 0.07 AUTHOR Map of HSV-1 helicase-primase in complex with a forked DNA AlphaFold in silico model RIGID BODY FIT emd_49582_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 385.2 385.2 385.2 90.0 90.0 90.0 X Y Z -0.7904444 1.0856684 0.00033502153 0.014304766 1.07 1.07 1.07 AUTHOR Sharp-map of HSV-1 helicase-primase in complex with a forked DNA emd_49582_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 385.2 385.2 385.2 90.0 90.0 90.0 X Y Z -0.10712184 0.2832769 0.0003236276 0.00840577 1.07 1.07 1.07 AUTHOR Half-Map-B of HSV-1 helicase-primase in complex with a forked DNA emd_49582_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 385.2 385.2 385.2 90.0 90.0 90.0 X Y Z -0.109355815 0.31391975 0.00032331666 0.00839592 1.07 1.07 1.07 AUTHOR Half-Map-A of HSV-1 helicase-primase in complex with a forked DNA