2025-09-24 REL RCSB INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-03-12 2025-09-24 2025-09-24 2025-09-24 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) DP2GM140920 United States Cryo-EM structure of a bacterial prototype ATP-binding cassette transporter MalFGK2. Qian R Jing W Vinay I Shanwen Z Jeehae S William GL Luis MRH Jong HS Young AG IIya L Kirill M Baron C Huan B bacterial prototype ATP-binding cassette transporter, TRANSPORT PROTEIN Ren Q Wang J Idikuda V Zhang S Shin J Ludlam WG Real Hernandez LM Zdancewicz S Kreutzberger AJB Chang H Kiessling V Tamm LK Jomaa A Levental I Martemyanov K Chanda B Bao H DeFrND: detergent-free reconstitution into native nanodiscs with designer membrane scaffold peptides. Nat Commun UK 16 7973 7973 2025 40858559 doi:10.1038/s41467-025-63275-8 2041-1723 9nqj FULLOVERLAP EMDB EMD-49659 associated EM volume
Cryo-EM structure of a bacterial prototype ATP-binding cassette transporter MalFGK2.
 ATP-binding cassette transporter MalFGK2 ATP-binding cassette transporter MalFGK2 0 1 2 3 Escherichia coli K-12 0.25 Maltose/maltodextrin import ATP-binding protein MalK Escherichia coli K-12 0.040979233999999996 2 Escherichia coli LEVO ASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKSTLLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVF QSYALYPHLSVAENMSFGLKLAGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEPL SNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKPLELYHYPADRFVAGFIGSPKMNF LPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQVGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIP SIRQNLVYRQNDVVLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGVA P68187 7.5.2.1 Maltose/maltodextrin transport system permease protein MalF Escherichia coli K-12 0.057052898 1 Escherichia coli LEVO MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAITTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVL FPLVCTIAIAFTNYSSTNQLTFERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGTLTNNQSGVKYRPNNQIGFYQSI TADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKPFLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVL LILPYAVPSFISILIFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLLKAIPDDLY EASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNGGPDRLGTTTPAGYTDLLVNYTYRIAFEGGG GQDFGLAAAIATLIFLLVGALAIVNLKATRMKFD P02916 Maltose/maltodextrin transport system permease protein MalG Escherichia coli K-12 0.032246226999999995 1 Escherichia coli LEVO MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNFATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFP VLLWLWNSVKVAGISAIGIVALSTTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVVFILSFIAAITEVPVASLLLRDV NSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITIVFLLAQRWLVNGLTAGGVKG P68183 MAGNESIUM ION 2.4305e-05 2 MG ADENOSINE-5'-DIPHOSPHATE 0.000427201 2 ADP VANADATE ION 0.00011493899999999999 2 VO4 singleParticle particle 2 8.0 GOLD 300 ETHANE 100 283.15 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.3 2.7 GATAN K3 BIOQUANTUM (6k x 4k) 1.172 1 NONE 3RLF 3.34 FSC 0.143 CUT-OFF 125454 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_49659.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 264.32 264.32 264.32 90.0 90.0 90.0 X Y Z -0.21528547 0.45798445 0.0010475696 0.0128582865 0.82600003 0.82600003 0.82600003 0.109 AUTHOR emd_49659_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 264.32 264.32 264.32 90.0 90.0 90.0 X Y Z -0.30528224 0.57190555 0.0007837857 0.029269932 0.82600003 0.82600003 0.82600003 AUTHOR emd_49659_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 264.32 264.32 264.32 90.0 90.0 90.0 X Y Z -0.2963298 0.56731087 0.00079344097 0.030276164 0.82600003 0.82600003 0.82600003 AUTHOR