2025-11-19 REL RCSB INITIAL_RELEASE REPOSITORY RCSB RCSB 2025-03-12 2025-11-19 2025-11-19 2025-11-19 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35GM152032 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) DP2GM150023 United States Composite structure of HSV1 helicase-primase in complex with a forked DNA and pritelivir He Q Baranovskiy AG Morstadt LM Babayeva ND Lim C Tahirov TH DNA replication, HSV-1 helicase-primase, VIRAL PROTEIN, VIRAL PROTEIN-DNA complex Baranovskiy AG He Q Suwa Y Morstadt LM Babayeva ND Lim CJ Tahirov TH Structural basis of herpesvirus helicase-primase inhibition by pritelivir and amenamevir. Sci Adv US 11 eadz1989 eadz1989 2025 41202142 doi:10.1126/sciadv.adz1989 2375-2548 EMD-49586 consensus EM volume EMD-49587 focused EM volume EMD-49588 focused EM volume 9nqp FULLOVERLAP EMDB EMD-49586 consensus EM volume EMDB EMD-49587 focused EM volume EMDB EMD-49588 focused EM volume EMDB EMD-49669 associated EM volume
Composite structure of HSV1 helicase-primase in complex with a forked DNA and pritelivir
 HSV-1 helicase-primase in complex with a forked DNA and pritelivir HSV-1 helicase-primase in complex with a forked DNA and pritelivir 0 1 2 3 4
HSV-1 helicase-primase in complex with a forked DNA and pritelivir sovled using cryo-EM single-particle analysis
Human alphaherpesvirus 1 strain 17 0.3 DNA replication helicase Human alphaherpesvirus 1 strain 17 0.09576080499999999 1 Escherichia coli BL21(DE3) LEVO GRAEAFLNFTSMHGVQPILKRIRELSQQQLDGAQVPHLQWFRDVAALESPAGLPLREFPFAVYLITGNAGSGKSTCVQTI NEVLDCVVTGATRIAAQNMYAKLSGAFLSRPINTIFHEFGFRGNHVQAQLGQYPYTLTSNPASLEDLQRRDLTYYWEVIL DLTKRALAASGGEELRNEFRALAALERTLGLAEGALTRLAPATHGALPAFTRSNVIVIDEAGLLGRHLLTAVVYCWWMIN ALYHTPQYAARLRPVLVCVGSPTQTASLESTFEHQKLRCSVRQSENVLTYLICNRTLREYARLSYSWAIFINNKRCVEHE FGNLMKVLEYGLPITEEHMQFVDRFVVPENYITNPANLPGWTRLFSSHKEVSAYMAKLHAYLKVTREGEFVVFTLPVLTF VSVKEFDEYRRLTHQPGLTIEKWLTANASRITNYSQSQDQDAGHMRCEVHSKQQLVVARNDVTYVLNSQIAVTARLRKLV FGFSGTFRAFEAVLRDDSFVKTQGETSVEFAYRFLSRLIFSGLISFYNFLQRPGLDATQRTLAYARMGELTAEILSLRPK SSGVPTQASVMADAGAPGERAFDFKQLGPRDGGPDDFPDDDLDVIFAGLDEQQLDVFYCHYTPGEPETTAAVHTQFALLK RAFLGRFRILQELFGEAFEVAPFSTYVDNVIFRGCEMLTGSPRGGLMSVALQTDNYTLMGYTYARVFAFADELRRRHATA NVAELLEEAPLPYVVLRDQHGFMSVVNTNISEFVESIDSTELAMAINADYGISSKLAMTITRSQGLSLDKVAICFTPGNL RLNSAYVAMSRTTSSEFLRMNLNPLRERHERDDVISEHILSALRDPNVVIVY P10189 3.6.4.- DNA primase Human alphaherpesvirus 1 strain 17 0.114558562 1 Escherichia coli BL21(DE3) LEVO MGQEDGNRGERRAAGTPVEVTALYATDGCVITSSIALLTNSLLGAEPVYIFSYDAYTHDGRADGPTEQDRFEESRALYQA SGGLNGDSFRVTFCLLGTEVGGTHQARGRTRPMFVCRFERADDVAALQDALAHGTPLQPDHIAATLDAEATFALHANMIL ALTVAINNASPRTGRDAAAAQYDQGASLRSLVGRTSLGQRGLTTLYVHHEVRVLAAYRRAYYGSAQSPFWFLSKFGPDEK SLVLTTRYYLLQAQRLGGAGATYDLQAIKDICATYAIPHAPRPDTVSAASLTSFAAITRFCCTSQYARGAAAAGFPLYVE RRIAADVRETSALEKFITHDRSCLRVSDREFITYIYLAHFECFSPPRLATHLRAVTTHDPNPAASTEQPSPLGREAVEQF FCHVRAQLNIGEYVKHNVTPRETVLDGDTAKAYLRARTYAPGALTPAPAYCGAVDSATKMMGRLADAEKLLVPRGWPAFA PASPGEDTAGGTPPPQTCGIVKRLLRLAATEQQGPTPPAIAALIRNAAVQTPLPVYRISMVPTGQAFAALAWDDWARITR DARLAEAVVSAEAAAHPDHGALGRRLTDRIRAQGPVMPPGGLDAGGQMYVNRNEIFNGALAITNIILDLDIALKEPVPFR RLHEALGHFRRGALAAVQLLFPAARVDPDAYPCYFFKSACRPGPASVGSGSGLGNDDDGDWFPCYDDAGDEEWAEDPGAM DTSHDPPDDEVAYFDLCHEVGPTAEPRETDSPVCSCTDKIGLRVCMPVPAPYVVHGSLTMRGVARVIQQAVLLDRDFVEA IGSYVKNFLLIDTGVYAHGHSLRLPYFAKIAPDGPACGRLLPVFVIPPACKDVPAFVAAHADPRRFHFHAPPTYLASPRE IRVLHSLGGDYVSFFERKASRNALEHFGRRETLTEVLGRYNVQPDAGGTVEGFASELLGRIVACIETHFPEHAGEYQAVS VRRAVSKDDWVLLQLVPVRGTLQQSLSCLRFKHGRASRATARTFVALSVGANNRLCVSLCQQCFAAKCDSNRLHTLFTID AGTPCSPSVPCSTSQPSS P10236 2.7.7.- DNA helicase/primase complex-associated protein Human alphaherpesvirus 1 strain 17 0.080005664 1 Escherichia coli BL21(DE3) LEVO MDTADIVWVEESVSAITLYAVWLPPRAREYFHALVYFVCRNAAGEGRARFAEVSVTATELRDFYGSADVSVQAVVAAARA ATTPAASPLEPLENPTLWRALYACVLAALERQTGPVALFAPLRIGSDPRTGLVVKVERASWGPPAAPRAALLVAEANIDI DPMALAARVAEHPDARLAWARLAAIRDTPQCASAASLTVNITTGTALFAREYQTLAFPPIKKEGAFGDLVEVCEVGLRPR GHPQRVTARVLLPRDYDYFVSAGEKFSAPALVALFRQWHTTVHAAPGALAPVFAFLGPEFEVRGGPVPYFAVLGFPGWPT FTVPATAESARDLVRGAAAAYAALLGAWPAVGARVVLPPRAWPGVASAAAGCLLPAVREAVARWHPATKIIQLLDPPAAV GPVWTARFCFPGLRAQLLAALADLGGSGLADPHGRTGLARLDALVVAAPSEPWAGAVLERLVPDTCNACPALRQLLGGVM AAVCLQIEETASSVKFAVCGGDGGAFWGVFNVDPQDADAASGVIEDARRAIETAVGAVLRANAVRLRHPLCLALEGVYTH AVAWSQAGVWFWNSRDNTDHLGGFPLRGPAYTTAAGVVRDTLRRVLGLTTACVPEEDALTARGLMEDACDRLILDAFNKR LDAEYWSVRVSPFEASDPLPPTAFRGGALLDAEHYWRRVVRVCPGGGESVGVPVDLYPRPLVLPPVDCAHHLREILREIE LVFTGVLAGVWGEGGKFVYPFDDKMSFLFA P10192 DNA (5'-D(P*AP*TP*CP*TP*GP*TP*T)-3') Homo sapiens 0.013114381 1 (DG)(DC)(DA)(DG)(DC)(DC)(DT)(DG)(DG)(DA)(DC)(DG)(DT)(DC)(DT)(DG)(DC)(DA)(DG)(DC) (DT)(DC)(DG)(DT)(DC)(DC)(DT)(DA)(DT)(DC)(DT)(DG)(DT)(DT)(DC)(DA)(DC)(DC)(DT)(DG) (DT)(DC)(DA) DNA Pritelivir 0.000402491 1 A1BXB ZINC ION 6.5409e-05 2 ZN water 1.8015e-05 1 HOH singleParticle particle 5.25 7.5 20.0 HEPES 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid 100.0 KCl potassium chloride 0.5 Glycerol Glycerol 2.0 DTT dithiothreitol 2.0 CHAPSO CHAPSO
CHAPSO is made fresh at 80 mM before being added to the sample at a final concentration of 2 mM immediately before vitrification
 ETHANE 95 277.15 FEI VITROBOT MARK IV
The sample was monodisperse
 TFS KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 1.0 2.5 81000.0 GATAN K3 (6k x 4k) 1 9216 50.0 1 3516384 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION From Cryosparc C1 3.2 FSC 0.143 CUT-OFF cryoSPARC 409125 MAXIMUM LIKELIHOOD cryoSPARC MAXIMUM LIKELIHOOD emd_49669.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 399.6 399.6 399.6 90.0 90.0 90.0 X Y Z -0.6476622 1.0277064 0.00016218101 0.018777529 1.11 1.11 1.11 0.09 AUTHOR Composite map of HSV-1 helicase-primase in complex with a forked DNA and pritelivir AlphaFold in silico model RIGID BODY FIT