Structure of MurJ in complex with single gene lysis protein from phage M

2026-03-11 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.pdbx_database_id_DOI _citation.title _citation.year _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.pdbx_database_id_DOI _citation.title _citation.year _em_admin.last_update DATABASE_REFERENCES citation citation_author em_admin _citation.pdbx_database_id_PubMed _citation.title _citation_author.identifier_ORCID _citation_author.name _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.pdbx_database_id_PubMed _citation.title _citation_author.identifier_ORCID _citation_author.name _em_admin.last_update RCSB RCSB 2025-03-19 2026-02-11 2026-02-11 2026-03-11 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM114611 United States The G. Harold and Leila Y. Mathers Foundation to W.M.C. United States Structure of MurJ in complex with single gene lysis protein from phage M Li YE Clemons WM lipid II flippase, phage single gene lysis proteins, peptidoglycan biosynthesis, TRANSPORT PROTEIN Li YE Antillon SF Baron GF Chamakura K Young R Clemons Jr WM Convergent MurJ flippase inhibition by phage lysis proteins. Nature UK 2026 41741639 doi:10.1038/s41586-026-10163-w 1476-4687 0006 NATUAS 9nu4 FULLOVERLAP EMDB EMD-49796 associated EM volume

MurJ in complex with single gene lysis protein from phage M MurJ in complex with single gene lysis protein from phage M 0 1 2 0.0066 soluble cytochrome b562, lipid II flippase MurJ fusion 1 1 Escherichia coli K-12 single gene lysis protein from phage M 1 2 Enterobacteria phage M Lipid II flippase MurJ Escherichia coli K-12 0.06666868 1 Escherichia coli BL21(DE3) LEVO MADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLA NEGKVKEAQAAAEQLKTTRNAYIQKYLLFSLAAVSIATMFSRVLGFARDAIVARIFGAGMATDAFFVAFKLPNLLRRIFA EGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAGMLAAPWVIMVTAPGFADTADKFALTSQLLKITFP YILLISLASLVGAILNTWNRFSIPAFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVL PRINFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPSGVLGVALGTILLPSLSKSFA SGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTVSLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYS RQDIKTPVKIAIVTLILTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVVAVLVMS GVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGSAWSHPQFEK P0AF16 Lysis protein Enterobacteria phage M 0.006625323999999999 1 Escherichia coli BL21(DE3) LEVO MGHHHHHHGSGSGSGSGSGSGSGSGSGSGSKYIINLAFCVLLLVAGDSIAYRVSQYLAPLVDTFTK K7QK87 singleParticle particle 4 8.0 20.0 C8H18N2O4S HEPES 150.0 NaCl Sodium chloride 5.0 C3H8O3 Glycerol 0.005 C47H88O22 LMNG 0.0005 C31H50O4 CHS Quantifoil R1.2/1.3 COPPER 400 CARBON HOLEY GLOW DISCHARGE 60 ETHANE 100 277.15 FEI VITROBOT MARK IV TFS KRIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 1.0 3.0 130000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K3 (6k x 4k) 70.0 1 cryoSPARC 4.5.3 PHASE FLIPPING AND AMPLITUDE CORRECTION 3.6 FSC 0.143 CUT-OFF cryoSPARC 4.5.3 61237 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_49796.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 332.8 332.8 332.8 90.0 90.0 90.0 X Y Z -0.29839304 0.44351688 0.00016922556 0.0053531434 0.65 0.65 0.65 0.06 AUTHOR ::::EMDATABANK.org::::EMD-49796:::: 6CC4 PDB experimental model AF-P0AF16-F1-v4 AlphaFold in silico model REAL emd_49796_msk_1.map emd_49796_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 332.8 332.8 332.8 90.0 90.0 90.0 X Y Z -0.06413177 0.15754667 0.00016922558 0.0028355252 0.65 0.65 0.65 AUTHOR ::::EMDATABANK.org::::EMD-49796:::: emd_49796_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 332.8 332.8 332.8 90.0 90.0 90.0 X Y Z -0.5751091 0.5678602 0.00014732927 0.061876044 0.65 0.65 0.65 AUTHOR ::::EMDATABANK.org::::EMD-49796:::: emd_49796_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 0 0 0 512 512 512 332.8 332.8 332.8 90.0 90.0 90.0 X Y Z -0.5664609 0.5525889 0.00014947247 0.062111404 0.65 0.65 0.65 AUTHOR ::::EMDATABANK.org::::EMD-49796::::