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    <admin>
        <current_status>
            <date>2026-02-11</date>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
        </current_status>
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        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-03-21</deposition>
            <header_release>2026-02-11</header_release>
            <map_release>2026-02-11</map_release>
            <update>2026-02-11</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)</funding_body>
                <code>R35NS132231</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Structure of Nanchung-Inactive-Calmodulin in complex with Nicotinamide, EDTA</title>
        <authors_list>
            <author>Fedor JG</author>
            <author>Lee S-Y</author>
            <author>Lee S-Y</author>
        </authors_list>
        <keywords>Membrane protein, membrane channel, TRANSPORT PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author ORCID="0000-0003-3660-3818" order="1">Fedor JG</author>
                    <author order="2">Kandasamy R</author>
                    <author ORCID="0000-0002-4739-1913" order="3">Park CG</author>
                    <author ORCID="0000-0003-1198-7309" order="4">Suo Y</author>
                    <author order="5">Weisel M</author>
                    <author ORCID="0009-0004-6952-2170" order="6">Rankl NB</author>
                    <author order="7">Nesterov A</author>
                    <author ORCID="0000-0002-0662-9921" order="8">Lee SY</author>
                    <title>Visualizing insecticide control of insect TRP channel function and assembly.</title>
                    <journal_abbreviation>Nat Commun</journal_abbreviation>
                    <country>UK</country>
                    <volume>17</volume>
                    <first_page>595</first_page>
                    <last_page>595</last_page>
                    <year>2025</year>
                    <external_references type="PUBMED">41392056</external_references>
                    <external_references type="DOI">doi:10.1038/s41467-025-67287-2</external_references>
                    <external_references type="ISSN">2041-1723</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>9nvp</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-49846</accession_id>
                <content_type>associated EM volume</content_type>
                <details>Structure of Nanchung-Inactive-Calmodulin in complex with Nicotinamide, EDTA</details>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>Nanchung-Inactive-Calmodulin</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Nanchung-Inactive-Calmodulin</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.7</theoretical>
                </molecular_weight>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Inactive</name>
                <natural_source database="NCBI">
                    <organism ncbi="286706">Halyomorpha halys</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.109381992</theoretical>
                </molecular_weight>
                <details>The 30-N-terminal residues are MGIIWGSGASSVNAGTVLDRVISQASNKDE. The authors were not able to assign the register of the residues in this region</details>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="9606">Homo sapiens</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)CLLYKLANYK
KGGELIDAYNAGGQSEVEKLIREQFGQLMYNEGKGALINRAEYLRWKFRDMQQVQIPIEASLSSQDPLSKWEDHQACWQM
QYRGSLGETLLHVLIICDTKIHTRLARTLLKCFPNLAIDVVEGEEYLGASALHLAIAYFNNELVQDLVEAGANVEQRAIG
SFFLPRDQQGQRPSKHTDYEGLAYLGEYPLAWAACCANESIYNLLLDNGANPDQRDTFGNMILHMVVVCDKLDMFGYALR
HPKMPASNGIANVAGLTPLTLACKLGRAKVFREMLELSAREFWRYSNITCSAYPLNALDTLLPDGRTNWNSALFIILNGT
KEEHLDMLDGGIIQRLLEEKWKTFARRQFLKRLVILMLHLICLSGAVYLRPTDRTKPLLGGDDWKSIARQGFEVATVLGV
LSYVLVQQGGEIRNQGFISFIKQLDPAKAIFLVSNILILVCIPFRLIDDKRTEEAILVFAVPGSWFLLMFFAGAVRLTGP
FVTMVYSMIVGDMFTFGIIYSIVLFGFSQSFYFLYKGFPGVKNTLYSSYHSTWMALFQITLGDYNYAELSHTSYPTLSKT
VFAIFMVLVPILLLNMLIAMMGNTYAHVIEQSEKEWMKQWAKIVVSLERAVNQEDCKQYLQEYSIKLGPGDDPSTEQRGV
MVIKSKSKTRAKQRKGAVANWKRVGKVTINELRKRGMNGEQLRRIMWDRASISTPVKVPQNPVVDVLMENTAEQQAQQTP
GSFGGALTAALDVMAFTHDLDLSASGITSSGLNAKELIVSDPFRDLVLSSEIDVGEEELATLAEAAVLSVMSGQSDESEK
LKIAKFNKQISFEQNAVLEVSDSDGFLDGQPLGQGSRARKVKSAQERQRAERTWGGTSISSIEEPPPYLPLPPPPPLHSH
QRPRPKTAKPNRVVPEAVVAKRPQSSALGDRVKSPPELLEPWSTRGIATINTILAWQPSDQDSM</string>
                    <external_references type="UNIPROTKB">A0A9P0MRC5</external_references>
                    <external_references type="UNIPROTKB">A0A9P0HI19</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Nanchung</name>
                <natural_source database="NCBI">
                    <organism ncbi="286706">Halyomorpha halys</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.101194867</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="9606">Homo sapiens</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MGNTESNVTSGVKKQADTSSIKIYKLVDLKGGGLLVELMKRAAQTKQYAELDHAIKTKVEPFLYNKGQGKMMPVSQLVLM
RNKERPRHKMLPPLRNLENPDDYDIESYVVPEPTEEDLKDPNKYREVCWDLKERGAVGETILHLCLLNATSLHADLAKRL
LRFYPKLINDVYMSDEYYGESVLHIAIVNEDPAMVKFLLDSGVNVNERCFGNFMCPEDQKASRTDSFDHEWVNLQSFTTY
EGYVYWGEYPLSFAACLGQEECYRLMLARGANPDNQDTNGNTVLHMLVIYSKIQTFDMAYEVGGDLSIRNVQYLTPLTLA
AKLARIELFFHILNIEREIYWQIGSITCAAYPLSQIDTIDIVTGNISKNSALNLVVFGEKDEHLELMDGVLIDLLNAKWN
AFVKFRFYRQFFLFLFYFLISLICFTLRPGPPPVKPISSINSTKPNGNNITADLSPENLTTLPKWIYKTEIKKYDPEDNT
SQNSRLNVILREVILTSLKVEDIDKVVSFSNAETPDNSREDAEGLVCNGNSIHSCIGNGSDVISVFGKGKSPKVPKPGAS
ADDFDYDDTWWEEFGQCRLLQVTSYIEMTRLISEVMLDIGALLYILAALREARFLGWSMFVENLMTAPSRVMFLFSCCLM
LTMPFLRFTCNEEIEDMMAVIIMLTTAPYFLFFCRGFKTVGPFVVMIYRMIMGDLLRFATIYLVFVMGFAQAYYIIFLSF
DNPLTPEGVDDSVSNPIPNPMEAVMAMFFMSMTSFGDYYPALERTAHEFCAKLCFVIYMAIVAILLVNMLIAMMGNTYQK
IAETRNEWQRQWARIVLVVERGVSPSERLTKLMWYSQPMSDGRRALVLRLNQSEEDKEEMKEILEMKRIHNRMVQKRKER
EM</string>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>Calmodulin-1</name>
                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.016852544999999997</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="9606">Homo sapiens</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT
DSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK</string>
                    <external_references type="UNIPROTKB">P0DP23</external_references>
                </sequence>
            </protein_or_peptide>
            <ligand macromolecule_id="4">
                <name>[(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate</name>
                <molecular_weight>
                    <theoretical units="MDa">0.0007179959999999999</theoretical>
                </molecular_weight>
                <number_of_copies>14</number_of_copies>
                <formula>6OU</formula>
            </ligand>
            <ligand macromolecule_id="5">
                <name>1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine</name>
                <molecular_weight>
                    <theoretical units="MDa">0.0007600759999999999</theoretical>
                </molecular_weight>
                <number_of_copies>6</number_of_copies>
                <formula>LBN</formula>
            </ligand>
            <ligand macromolecule_id="6">
                <name>NICOTINAMIDE</name>
                <molecular_weight>
                    <theoretical units="MDa">0.000122125</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <formula>NCA</formula>
            </ligand>
            <ligand macromolecule_id="7">
                <name>(2~{S})-2-azanyl-3-[[(2~{R})-3-hexadecanoyloxy-2-[(~{Z})-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-propanoic acid</name>
                <molecular_weight>
                    <theoretical units="MDa">0.000762006</theoretical>
                </molecular_weight>
                <number_of_copies>2</number_of_copies>
                <formula>D39</formula>
            </ligand>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">2.6</concentration>
                    <buffer>
                        <ph>8.0</ph>
                        <component>
                            <concentration units="mM">20.0</concentration>
                            <formula>Tris</formula>
                            <name>Tris</name>
                        </component>
                        <component>
                            <concentration units="mM">150.0</concentration>
                            <formula>NaCl</formula>
                            <name>NaCl</name>
                        </component>
                        <component>
                            <concentration units="%">0.02</concentration>
                            <formula>GDN</formula>
                            <name>GDN</name>
                        </component>
                        <component>
                            <concentration units="%">5.0</concentration>
                            <formula>glycerol</formula>
                            <name>glycerol</name>
                        </component>
                        <component>
                            <concentration units="mM">20.0</concentration>
                            <formula>NAM</formula>
                            <name>Nicotinamide</name>
                        </component>
                        <component>
                            <concentration units="mM">5.0</concentration>
                            <formula>EDTA</formula>
                            <name>EDTA</name>
                        </component>
                    </buffer>
                    <grid>
                        <model>Quantifoil R1.2/1.3</model>
                        <material>COPPER</material>
                        <mesh>300</mesh>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                            <film_topology>CONTINUOUS</film_topology>
                            <film_thickness>2.0</film_thickness>
                        </support_film>
                        <pretreatment>
                            <type>GLOW DISCHARGE</type>
                            <time units="s">30</time>
                            <atmosphere>AIR</atmosphere>
                            <pressure units="kPa">0.00037</pressure>
                        </pretreatment>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">100</chamber_humidity>
                        <chamber_temperature units="K">280</chamber_temperature>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                    </vitrification>
                    <details>Monodisperse sample</details>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">100.0</c2_aperture_diameter>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_defocus_min units="µm">1.0</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.2</nominal_defocus_max>
                    <nominal_magnification>81000.0</nominal_magnification>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <temperature>
                        <temperature_max units="K">70.0</temperature_max>
                    </temperature>
                    <alignment_procedure>
                        <coma_free/>
                    </alignment_procedure>
                    <specialist_optics>
                        <energy_filter>
                            <name>GIF Bioquantum</name>
                            <slit_width units="eV">20</slit_width>
                        </energy_filter>
                    </specialist_optics>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 (6k x 4k)</film_or_detector_model>
                            <digitization_details>
                                <dimensions>
                                    <width units="pixel">5760</width>
                                    <height units="pixel">4092</height>
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                            </digitization_details>
                            <number_grids_imaged>1</number_grids_imaged>
                            <number_real_images>10870</number_real_images>
                            <average_exposure_time units="s">2.4</average_exposure_time>
                            <average_electron_dose_per_image units="e/Å^2">60.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <particle_selection>
                    <number_selected>2660046</number_selected>
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                <ctf_correction>
                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                            <version>4.0</version>
                        </software>
                    </software_list>
                    <type>PHASE FLIPPING AND AMPLITUDE CORRECTION</type>
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                <startup_model type_of_model="NONE"/>
                <final_reconstruction>
                    <number_classes_used>1</number_classes_used>
                    <algorithm>FOURIER SPACE</algorithm>
                    <resolution units="Å" res_type="BY AUTHOR">2.85</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
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                <initial_angle_assignment>
                    <type>ANGULAR RECONSTITUTION</type>
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                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.5661978</minimum>
                    <maximum>1.598036</maximum>
                    <average>0.005329458</average>
                    <std>0.06781015</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">1.08</x>
                    <y units="Å">1.08</y>
                    <z units="Å">1.08</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-49846::::</label>
                <annotation_details>half map 1</annotation_details>
            </half_map>
        </half_map_list>
    </interpretation>
</emd>
