Structure of the small subunit of the flowering plant mitoribosome with the maturation factor RsgA

2026-03-18 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2024-04-02 2026-03-18 2026-03-18 2026-03-18 Alexander von Humboldt Foundation Germany Swiss National Science Foundation 210561 Switzerland Structure of the small subunit of the flowering plant mitoribosome with the maturation factor RsgA Waltz F Skaltsogiannis V Giege P mitochondria, mitoribosome, assembly factor, plant, RIBOSOME Skaltsogiannis V Wolff P Nguyen TT Corre N Pflieger D Blevins T Hashem Y Giege P Waltz F Structural insights into cauliflower mitoribosome in translation state and in association with a late assembly factor. Nat Commun UK 16 10839 10839 2025 41330901 doi:10.1038/s41467-025-65864-z 2041-1723 EMD-50015 focused EM volume

head focused

EMD-50017 focused EM volume

body focused

EMD-50016 focused EM volume

RsgA focused

9evt FULLOVERLAP EMDB EMD-50014 associated EM volume

Structure of the small subunit of the flowering plant mitoribosome with the maturation factor RsgA

EMDB EMD-50015 focused EM volume

head focused

EMDB EMD-50017 focused EM volume

body focused

EMDB EMD-50016 focused EM volume

RsgA focused

Small subunit of the flowering plant mitochondrial ribosome in presence of RsgA Small subunit of the flowering plant mitochondrial ribosome in presence of RsgA 0 1 2 3 4 5 6 7 8 9 10 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 Brassica oleracea var. botrytis Ribosomal protein S2 Brassica oleracea var. botrytis 0.023743544999999998

GWHPBJSH046906

1 LEVO MTLHGAVIQKLLNTGSHLGRRAAEHHFKQYAYGTRNGMTIIDSDKTLICLRSAASFVANLASARGNIFFVNTNPLFDEIV ELTSRRIQGDAYNHNRSTNLWKMGGFLTNSYSPKKFRSRHKKLCFGPTTMPDCVVVFDAERKSSVVLEAAKLQIPVVAIV DPNVPLEFFEKITYPVPARDSVKFVYLFCNVITKCFVAEQMKMGIKDGDLAA A0A0D3CN01 Small ribosomal subunit protein uS3m Brassica oleracea var. botrytis 0.064731312

GWHPBJSH000741

1 LEVO MARKGNPISVRLGKNRSSDSSWFSDYYYGKFVYQDVNLRSYFGSIRPPTRLTFGFRLGRCILLHFPKRTFIHFFLPRRPR RLKRREKTRPGKEKGRWWTTFGKAGPIGCLRDDTEEERNEVRGRGARKRVESIRLDDRKKQNEIRGWPKKKQRYGYHDRS PSIKKNLSKLLRISGAFKHPKYAGVVNDIAFLIENDDSFKKTKLFKFFFPKKSRSDGPTSYLRTLPAVRPSLNFLVMQYF FNTKNQMNFDPVVVLNHFVAPGAAEPSTMGRANAQGRSLQKRIRSRIAFFVESLTSEKKCLAEAKNRLTHFIRLANDLRF AGTTKTTISLFPFFGATFFFLRDGVGVYNNLDAREQLLNQLRVKCWNLLGKDKVMELIEKFKNLGGIEELIKVIDMMIEI ILRKRGIPYRYNSYFYEVKKMRSFLSNRTNTKTLIESVKIKSVYQSASLIAQDISFQLKNKRRSFHSIFAKIVKEIPKRV EGIRICFSGRLKDAAEKAQTKCYKHRKTSCNVFNQKIDYAPVEVSTRYGILGVKVWISYSQKKGGRAISETYEI A0A068BCX1 Ribosomal protein S4 Brassica oleracea var. botrytis 0.043438488

GWHPBJSH000696

1 LEVO MWLLKKLIQRDIDMSPLRFQTCRLLLGNVWNRELTIIQRRILRRLRNRKRSIKKRKIYSKKYLTSYIQLQTTRKLSLFYG DLPITEMHRGTKRTSYIPFLLNLETRFDVILLRLHFLETIPQARQLISHRRVCVNKGMVSITHLKLSHGDIISFQENNAI IRGEEIRRSFYKEILVEKIIGKLLHQPLRMWRRSKTEWFHLLKTKRGCRLLLKSRFLQQLRSSMQEEDLERTKKFGSEKV CLGSSFAEHKRMKRNLLKSLFLSKRRKEKNLNLPTRTISPIVYNSSLSLYSNSTYCFASPHKLTMKRRIKRIELPTHYLE VNYRTPKAVVFYGPNIGHIPHDIRLKDLNLLLWSRNGRGQNI Q6YSL2 S5 DRBM domain-containing protein Brassica oleracea var. botrytis 0.058296727

GWHPBJSH015007

1 LEVO MSFRTRASSWTRLLLRRNPRSSFSRQISSLDASGSAFPGRITPWISSQTHRFSSATTISPAEADRVVRDLLAEVEKEKQR EREERQRQGLDCKDIDDEDEDEEDYLGIEPFIEKLKKQNLKDDGELNRREESSDSDSELDEVDWDEERKKEDMFNKKFQR HKELLQTLTKSETLDEAYKWMTKLDKFEEKHFKLAPEYRVIGELMNRLKVAEGKDKFILQQKINRAMRLVEWKEAFDPNN PANYGVIERDNVQGGEEKEERLVADGSKDDNDDNEEEFDDMKERDDILLEKLNAIDKKLESKLSELDHTFGKKGKRLEEE IRDLAEERNALTEKKRQPLYRKGYDVHVIDVKKVAKVTKGGRVERYTALMVCGNYEGVIGYAKAKAETGQSAMQKAYEKC FQNLHYIERHEEHTIAHAIQTSYKKTKLYLWPAPTTTGMKAGRVVKTMLLLAGFKNIKSKVIGSRNSYNTVKAVLKALNA VETPKDVQEKFGRTVVEKYLL A0A0D3AS28 bS6m Brassica oleracea var. botrytis 0.016011446999999998 1 LEVO MPLYDCMLLFKPIIRKEGLIDLVARIGKHVYSKNGVLTEVKSFGKVELGYGIKKLDGRHYQGQLMQITMMATPNINKELH YLNKDDKLLRWLLVKHRDIKIGSSEMEDRQDDSNRVTNRSLYDESSTDEDDDILGLSR A0A0D3CIR5 uS7m,Small ribosomal subunit protein uS7m,Small ribosomal subunit protein uS7m Brassica oleracea var. botrytis 0.017955059

GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704,GWHPBJSH000704

1 LEVO (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)GGLDGEQKLLIKKLVNFRMKEGKRTRVRAI VYQTFHRPARTERDVIKLMVDAVENIKPICEVAKVGVAGTIYDVPGIVARDRQQTLAIRWILEAAFKRRISYRISLEKCS FAEILDAYQKRGSARRKRENLHGLASTNRSFAHFRWW 40S ribosomal protein S15a Brassica oleracea var. botrytis 0.014874127

GWHPBJSH002412

1 LEVO MGRRILNDALRTIVNAERRGKASVELKPISTVMSSFLRIMKEKGYIKNFQVHDPHRVGRITVDLQGRVNDCKALTYRQDV KAKEIEEYTERTLPTRQWGYVVVTTPDGILDHEEAIKRNVGGQVLGFFY A0A398AJL3 30S ribosomal protein S9 Brassica oleracea var. botrytis 0.042328410000000004

GWHPBJSH080139

1 LEVO MLSRLFLRSSNLRLATLVSSKSNSQIFSSFIRPLSTNSSGGGGGNDSGNGGNRNDAPWSFSGVNDGKSDPFSSFESGSPG GDGKWPKEEPKRWNMKEEGDEKAVFGDVSNGFGDVKSSGWDVSSKPWDLKEDEEDGKVVFDTSGEMPVSFDNSLVNEEEE RAKKELFEKEEKELTEVIKGPDRAFGDLIAKSGITDEMLDSLIALKDFQGVQGLPPLTEIENLRREKSSKKSSRAEIELQ MQEEIAKARVRQVDEAGRAYGTGRRKCSIARVWIVPGKGKFLVNDKEFDVYFPMLDHRAALLRPLAETKTLGSWDINCTV TGGGTTGQVGAIQLGISRALQNWEPDMRTALRAAGFLTRDSRVVERKKPGKAKARKSFQWVKR Small ribosomal subunit protein uS10 domain-containing protein Brassica oleracea var. botrytis 0.025251247999999997

GWHPBJSH050210

1 LEVO MIGVLRRSSLPSRHALSAALTSFDSGLSLNITSAATGASVKLNLVGNGSTLSSASFVISARDFHIRSEPSMVSPAKISFR GYATGSESSIGDSAKIKISPENVKPLSKKKKGGGESTSKIKGTRICIAIRSFDNPEKEAWCLPPHSRYAAMPDTRTLYTV LRSPHVDKKSREQFEMRFKKRFLVIKAQSHELSNKLFWLKRYRILGAQYELQFHCKTRLDMSPVLGSK A0A0D3CHA1 Ribosomal protein S11 Brassica oleracea var. botrytis 0.033108223

GWHPBJSH051931

1 LEVO MNGVSRHLRASSLPSLIRSYGGIKSVCRFSCQSDGSSGGRFGEQGRVPGDSENKSGLPDPNASPSKSFSEMRSGLLNKIG DGGYSAPNAPPTFRNPLRSRLPNTLPSQSGQMNQEGLPNTGGTGFSAPSYQSFTQSNLLKDNFRTGGISKDLDFVRGVIE DDEGRRATGLFSHFHRPNLETNADIIHIKLLRNNTFVTVTDSKGNVKCKATSGSLPDLKGGRKMTSYTADATAENIGRRV KAMGLKSVVVKVNGFTHFKKKRNAIVAFRSGYSNSRNDQNPIVYIEDTTRKAHNGCRLPRKRRV A0A3P6EIC7 Small ribosomal subunit protein uS12m Brassica oleracea var. botrytis 0.014294817999999999

GWHPDUBS019876

1 LEVO MPTFNQLIRHGREEKRRTDRTRALDKCPQKLGACLRVSTRTPKKPNSALRKIAKVRLSNRHDIFAYIPGEGHNLQEHSQV LIRGGRVKDLPGVKFHCIRGVKDLMGIPGRRRGRSKYGAEKPKSI P48857 uS13m Brassica oleracea var. botrytis 0.017398300999999998

GWHPBJSH064799

1 LEVO MLGLRRSAATLFDHSQSLLRNLSFHGLRVQGIRVGNAEVPNHKPLKTGLQEVYGIGRRKSHQVLCGLGITNKLARDLTGK ELIDLREEVGMHQHGDELRRRVGSEIQRLVEVDCYRGSRHRHGMPCRGQRTKTNARTKKGKRVAIAGKKKAPRK A0A0D3D1F0 Small ribosomal subunit protein uS14m Brassica oleracea var. botrytis 0.017928849999999996

GWHPDUBS019475

1 LEVO MANLRGVLTNVSSYCQRSFSQSALLWRSAVKPPNNLAQIQSRQISTSLAKHSGTEQGVKRNSADHRRRLLAARFELRRKL YKAFCKDPELPSEMREKNRYKLSKLPRNSAFTRIRNRCVFTGRSRSVTELFRMSRICFRGLANKGELVGIKKSSW A0A3P6BZM6 Small ribosomal subunit protein uS15c Brassica oleracea var. botrytis 0.047211241999999994

GWHPBJSH065138

1 LEVO MAFHLARRKQRLHSNPYLIHLFSTSSSSASPQDGNESGEQQPSQSPSDFKISSYFSGIKSSLKQQPQHQAQDGRRQLAGF DPKAPSFSGSGGDFQDVRRNLNEFRRRAAAPPARDLQDLYKQNVLSKSGDLGAGKVEGSPFENLKKNLRQMRPQETRWSN LSSLQSIMKAKDSGNVRSNVFGGGEGLPISVFGEEIEERKKMGDDSEEMKSEFIKSYNTKELGEILRQYRPEGKREEGWF SLQELNQRLVKLREVEEAAAQGARKGIAFDDLRSEIQQARKSQAFQNLDFFSVLNGTPKYLLEPPKDELVQTYFHPDNMS SAEKMKIELAKVREEFKMSESDCGSARVQVAQLTTKIKHLSSVLHKKDKHSKKGLIAMVHRRKKLLKYMRRTDWDSYCLS LSKLGLRDNPDYKF A0A0D3D1Z7 30S ribosomal protein S16 Brassica oleracea var. botrytis 0.015330756999999999

GWHPBJSH018119

1 LEVO MVVRLRLSRLGCKNRPFFRVMATDSRSPRDGKHIEVLGYFNPLPGQDGGKRMGLKFDRIKYWLSVGAQASDPVQRLLFRS GLLPPPPMVAMGRKGGERDTRPVDPMTGRFVDAEKKTPVIIDNQPKEEDNAQDKSP A0A0D3ALM2 Small ribosomal subunit protein uS17c Brassica oleracea var. botrytis 0.012090024

GWHPBJSH049400

1 LEVO MKPVIGTVVSNKMQKSVVVAVDRLFHNKLYNRYVKRTSKFMAHDETNACNIGDRVKLDPSRPLSKHKNWIVAEIIKKARI YSPQAAAAAALSFSAAKSPSESSVPPVSSS A0A3P6EMJ2 Small ribosomal subunit protein bS18c Brassica oleracea var. botrytis 0.027106116999999996

GWHPBJSH076274

1 LEVO MKLAHSAIRSLSDVASFQTCRPFVSRSLSTNANQDDWMFGGKDEKANSFFQHLGKAEKDKRDYTGFSRSQGNGSRFDPSS DGVDGKLKEAALIYNVDDDEGVKDGYSFRPDVNSWGVNQFPRDMNFRRQMQRPRQNNKAEITTEEVLKKADFRNVRFLAQ FITEAGILVKRKQTGISAKAQRKIAREIKTARAFGLMPFTTMGTKAFTFGKTMENRDQDFEYEVIDDDDEYDDNTPE A0A078FCZ9 Small ribosomal subunit protein uS19m Brassica oleracea var. botrytis 0.023675223000000002 1 LEVO MAFCTKLGGHWKQGVNVPVSSMLGSLRYMSTKLYIGGLSPGTDEHSLKDAFSSFNGVTEARVMTNKVTGRSRGYGFVNFI SEDSANSAISAMNGQELNGFNISVNVAKDWPSLPLSLDESIEEAEKKENKMMSRSVWKDPFVDAFLMKKKNAALNRKIWS RRSTILPEYVDSAVRIYNGKTHVRCKITEGKVGHKFGEFAFTRKVKKHAKAK P39697 Ribosomal protein S21 Brassica oleracea var. botrytis 0.011541774

GWHPBJSH012122

1 LEVO MNTIAKRVTGLLTRPSHSQLQQERGIRVKVFSGDLDKALTILQRKMQSSGMERLIKAQQTHHIKNSEKKVLARKNLERKI KSIDFARKLQSILIKKVRGL A0A0D3AW14 30S ribosomal protein S31, mitochondrial Brassica oleracea var. botrytis 0.010524609

GWHPDUBS022287

1 LEVO MMAAAQWCSSMTRLVMMMQRTSPALACSARYSSLCPAAASMEVCGRGDSKTKKGKRFKGSYGNSRGKKQKMIERIKDKLE LPRSTPWPLPFKLI A0A0D3C0Z4 Small ribosomal subunit protein mS23 Brassica oleracea var. botrytis 0.021934494

GWHPBJSH071753

1 LEVO MSWMKGDLLSKTRRLVGGLATREPVWLKAMEASPPPVFPRSNGNLKKIVLPEDPYVRRFARKHPEAKLVEPNKVSAFIPD QARVYGCRVLELTKNGISEDDAMSVANMEYLAERKEKKKAYKRLKELAVLQDKAPPPKPYLSTKKEMQTQEKKPPTDPPS VRRLVNQLKQQKDVLLQDKPGGSANQDHWIDE A0A3P6DYP2 mS26 Brassica oleracea var. botrytis 0.023093625

GWHPBJSH068774

1 LEVO MSLCNQLSRLVKQSPLTKQSLRSFSAEAATHHRHHQETHSFLEPESYIGSWEAPKDPKDAERMLAQLRRDYAKKVSLYRK DYVHEIEMLRVEKQRKDEARLVAERAANEERRRLKAEAAKVRAEERKIFQDEFRKTLMKERAEKLEFWRMTGLKREEKKK ARKKLLHEQSSLWIEPKELEKKITEALVDSTTL A0ABQ7YPD6 Small ribosomal subunit protein mS29 Brassica oleracea var. botrytis 0.054288593999999996

GWHPBJSH054439

1 LEVO MFRSVVRITSRNFNLNRRSLIPNDAVSHFPSPPSASAATLIPSLDHAHRRRFSSSKSTKSSITKTKKSEGKKSKSKGGES GAAAGAEDGEFGGSAGDDLEAGRAKRLADDEKTPSLDVGPNGRPLFTPRDVTLSKLSQKDIGSYFNFDEAALKAVLPEGL ASGIEDEFKESWRPALLVRKSFLDLRDNFRRIADPPMWPSEGKGGVKPKKQIILDGPVKSGKSIALAMLVHWARDEGWLV LYAPKGRDWTHGGYFYKNQHTGFWDTPLQAESILKDFVKFNEPRLRELRCNVYDPIVLGEGAGVGYLKGQETMPIPEDST LYDLVQMGINSTHAAVSVVVRLRKELSLVKDVPVLIAIDQYNNWFTFSEFEEPVTPRSCRPIHARELTTVNAFRSMMHDD MMVGAFSHSTAVGKLRKDLPDVPADARQNFPRYSLDEAEAVCYYYLRQRLVRREVFSEENWKKIYYLANGNGAEMRWLVP FMR A0A0D3CAV8 BnaC06g06470D protein Brassica oleracea var. botrytis 0.05347624199999999

GWHPBJSH057126

1 LEVO MRGAIGRRLSNPSNGFSLASIVKHTPFLVQPTSHFSSSSSDGGAVRGRGRGRGGSGSPAAGAGQFGFNREPEKANEPVGQ ARGSSESQSPGGYGHGRGRPIQSDPISPPFSSFVKPDSHSVGRGRGSLRSDPVSPFSPEPPRHPAPPQPQQPRFEPAKDG VQGSPPFAKLEATKDATSPPPPPPPGAPNNISNALGSGAGRGKPFVQNEDNRHIQRSPQPLQRQKRVQPPKDTTPRPQLS PEEAGRRARSQLSRGEAEGGSGGGVRGRGGGRGRGRGARGRGRGRGGEGWRDDKKEEEAEQEALSVFVGDNADGEKFAKK MGDEIMAKLAEGYEDICERALPSTAHDALVDAYDTNLMIECEPEYLMPDFGSNPDIDEKPPMPLRECLEKVKPFIVALEG IKDQEEWEEAIGEAMAEAPRMKEIVDHYSGPDRVTAKKQNEELDRIATTVPQSAPDSVKRFADRVALSLKSNPGWGFDKK YQFMDKLVLEVSQNYK A0A078I5F5 MOSC domain-containing protein Brassica oleracea var. botrytis 0.011631558999999998

GWHPDUBS036572

1 LEVO MASGSLKNLVTSAVTVGVTEARARIFGHMLNPTGQRSPHKILRKKLFGDKVAEWYPYDIKNEDPNVLAREEKERLSKLEM LKRRNKGPPQKGHGRRAAKRNK A0A3P6ERU1 Mitochondrial 28S ribosomal protein S34 Brassica oleracea var. botrytis 0.016650186

GWHPBJSH018393

1 LEVO MAATLMNRAISRTETVGAFRLSLNLLRNFSAPAAAAAASPSTENPSSDSIKPKRRKKKNLIEVAQFLPNWGIGYHMAKAH WNGVSYEITKINLYKDGRHGKAWGIVHKDGLRAAEAPKKISGVHKRCWKYIPNLSKTTPATTGSAPAAEVQAA A0A8S9KXA0 EngC GTPase domain-containing protein Brassica oleracea var. botrytis 0.055785577999999995

GWHPBJSH063220

1 LEVO MQISSLSIIRHSSPFLLRRASIHHGGCGVRLGTGIGIRRSFHLLTLSARRDNPDVSRKPHPSKNMLRAKHIGKDHSSLAP VLSPDHNPSLLPSQAIGTVATAQANFMRVVVQDGDGDGDDRSSSTSSSKDGVELLCVVRAVLKKIRRRVLVGDKVLVGSI DWVDRRGMIENVFQRRSEILDPPVANVDHLLVLFSLDQPKLEPFTLTRFLVEAESTGIPLTLALNKCELITEEELESWKM RLRGWNYEPFFCSVGTKEGLDAIAFVLRNQTSVIVGPSGVGKSSLINILRSSSGGGDVEDENWFEPIIGNKWFEDQRVGE VSTRSGRGKHTTRNVSLLPITEGGYLADTPGFNQPSLLKVTKHSLALCFPEIRKMIEEEKCGFKDCLHIGEPGCVVKGEW ERYPYYLQLLDEIRVREEFQLRTFGTKREGDVRYKVGGMGVKQAEPRLMPKKHRRESRKKVKQTMISELDEYEDEESDLD IEDDPIVQAIENEKER A0A0D3CYN0 Small ribosomal subunit protein mS35 mitochondrial conserved domain-containing protein Brassica oleracea var. botrytis 0.048589311999999996

GWHPBJSH049245

1 LEVO MRGALFRNASLCARRLLLSPRINTHQPSSSSNARFVAPIATPFPPRLFSSESDSSGENPPPPPESSSPIESSNKKDLAVE DVGNKELKARIEKYFNEGNEDALPGIIEALLQRRLADKHADTDDEVMDSLQNQPFKDDVKDEDFESDFEEAHSTDDELED LYNSPEYVKKKMQNNEFFNMDEKKWDVIVRDGIRHGILKDTKECEEILEDMLHWDKLLPDDLKKKVEAKFNELGDMCERG EIEPEAAYELFKEFEDEMVIQYGDQMEAAGPPKFDETDPSYSNTNLDDPPGKGPILRWQSRIVFAPGGDAWHPKNRKVKL SVTVKELGLSKHQARRLRELVGKRYDSGKDELTITSERFEHREENRKDCLRTLYGLIEEAAKANKIAEDIRTAYVKQRLQ ANPAFMQKLQAKIMRSKESNPINA A0A3P6FE97 IMS import disulfide relay-system CHCH-CHCH-like Cx9C domain-containing protein Brassica oleracea var. botrytis 0.009227926 1 LEVO MGRKAGNLYINPKKLGNLAKPCMKEMVSFLNCMALNNIKDDKCEKQKQLLSVCMQGQSDHKNKSWGNINYHLQRLTRGRK A8MRX3 Small ribosomal subunit protein mS38 Brassica oleracea var. botrytis 0.014562170999999999

GWHPDUBS042575

1 LEVO MANLMQRFIRNQSCLRAIPKLTPNLIPHQKPCIIINNNVESPFPLTNPVIAFSGSGIKPDEALRFYPSFPIGYGLNPSVV HGSGLTDTVVEEKEKEVVIHADSVKKKRKKKMNKHKYRKLRKKQGRKS A0A3P6GL56 IGR domain-containing protein Brassica oleracea var. botrytis 0.01249895

GWHPBJSH068514

1 LEVO MAWMQMIQSARFLRKTQPFSTPSLARFYSKPAPYAVKVGIPEFLSGIGGGVETHVARLETELGDLSKLLVTRTLKLKKFG IPCKHRKLILKYSQKYRLGLWKPRADAIKA A0A0D3DCJ8 mS45 Brassica oleracea var. botrytis 0.044013531

GWHPBJSH025481

1 LEVO MFKLSRMIPRLQSVSLNPSTTASGSLIENAPRIFQSLGNRAFSGKSSSDGSGSNENGWDIATGGSFGGGTTGGSDDLDWD HKSMWSTGLSKEHFDGVSVGRQNPSSENAVSDSGDVMSKLGPREVAMVNEMNEYDDMIKEIEKDNRHSRVFVDGIKQKMM EMSVLLKQVKEPGARGSYLKDSEKTEMYRLHKENPEVYTVERLAKDYRIMRQRVHAILFLKEDEEEEERKLGRPLDDSVE RLLDEYPEFFVSHDREFHVASLSYKPDFKVMPEGWDGTIKDMDEVHYEISKKEDDILYEEFLRRFEFNKLKWKGEVKCHK YSRRRSSDGWKITVEKLGPQGKRGNGGGWKFVSLPDGSSRPLNEVEKMYVKREAPRRRRKILP A0A0D3BFU2 mS47 Brassica oleracea var. botrytis 0.045518668

GWHPBJSH021410

1 LEVO MQTVRALRRVTKPSQWVRSVSQGKRSFSALPNFSASDADVQDQVSVEGKAKSRAAILDRPSSLNALSAPMVGRLKRLYES WEENPAISFVLMKGSGKTFCSGADVLPLYHSINEGNTEECKHFFGSLYNFVYLQGTYLKPNIAIMDGVTMGCGGGISIPG MFRVATDKTVLAHPEVQIGFHPDAGASYYLSRLPGYLGEYLALTGQKLDGVEMIACGLATHFCLHSRLGMVEERIGKLLT DDPTVIEASLAQYSDLVYPDNTSVLHKIEMIDRYFGLDTVEEIIEAMENEVADSGNEWCKKTLKQVKEASPLSLKITLQS IREGRFQTLDQCLTREYRISLCGVSKTVSGDFCEGIRARLVDKDFAPKWDPPRLEDVSKDMVDCYFSPATDADDSESELK LPTAQREPYF A0ABQ7BUT9 (rape) hypothetical protein Brassica oleracea var. botrytis 0.043364805

GWHPBJSH050066

1 LEVO MQYLSNSRRIARALKPLALLEADVRFASHVVDSTQWQNHRSPSVSVSPGMNTQSHMTGLMMLRSSFSSEAKLVEDPTEAV KELHGKILDSVNVKRSMPPNALLWSLIENCRKEDDISFLFDALQNLRRFRLSNLRIHDNFNCNLCRQVAKTCVRVGAINH GKRALWKHNVHGLTPSVASAHHLLSYALEHKNSNLMDEVMKLLKANDLPLQPGTADLVFRICHETDSWDLLAKYSKKFCK AGVKLRKTTFDVWMEFAAKRGDTESLWKVDKLRSETYTQHTLSAAFSCAKGFLLEHKPEEAAAVIQIICQAYPDEKKSAL EAEFKKLVNEWPVDVLKHQNEEDKKAVAASLKSDIPAMVNALVNSGLRVSVDLDELNKNEALLS A0ABQ7ZIA1 PROP1-like PPR domain-containing protein Brassica oleracea var. botrytis 0.08264882 1 LEVO MKSFLLSRQAIHRISLLSSKTPTFCRNFSAITSTISHSDRHLRSYDEQTPFQNVEIPRPISSFNRYFHFTRESRLSESSA AIDDSNDQEEDDEDGTTNEFLSRFVWIMRGKVSEAYPDCDKKMIDGMLLLIVEKVVEEIERGGFNKVGSAPPSPSSEFSD DLWATIWEVSNTVLKDMEKERKKEKMKQYVQSPEVMEMCRFAGEIGIRGDLLRELRFKWAREKMDDAEFYESLEQQRDLD NSIRESETVDGEVEEEGFVPSDEVESRSISLPKRKGKLKYKIYGLELSDPKWVEMADKIHEAEEEADWREPKPVTGKCKL VMEKLESLQEGDDPSGLLAEWAELLEPNRVDWIALINQLREGNTHAYLKVAEGVLDEKSFNASISDYSKLIHIHAKENHI EDVERILKKMSQNGIFPDILTATALVHMYSKSGNFERATEAFENLKSYGLRPDEKIYEAMILGYVNAGKPKLGERLMKEM QAKELKASEEVYMALLRAYAQMGDANGAAGISSSMQYASDGPLSFEAYSLFVEAYGKAGQVDKAKSNFDEMRKLGHKPDD KCIANLVRAYKGENSLDKALRLLLQLEKDGIEIGVITYTVLVDWMANLGLIEEAEQLLVKISQLGEAPPFELQVSLCCMY SGVRNEKKTLQALGVLEAKRDQMGPNEFDKVISALKRGGFEKDARRMYKYMEARKFLPSQRLQMDMVAPPRAFGSGSGRV RRFNS A0A654ECT9 RRM domain-containing protein Brassica oleracea var. botrytis 0.016001051

GWHPBJSH080930

1 LEVO MHYMGLFSRAGNIFKQPRAMQAANAMLQGNLSLTPSKLFVGGLSPTTDVDILKEVFGKFGKIVDVVVVSDRETGVSKGFG FVTYDSIDAANKAMQQMNDQELDGRIIGVNPADSGGGGGGFARRGGYGGGRGGFGRGGFGRGGFGRGGYNFVR A0A3P6FEP3 18S rRNA Brassica oleracea var. botrytis 0.504947344 1 CAUAGUCAAAAGAAGAGUUUGAUUCUGGCUCAGAAGGAACGCUAGCUAUAUGCUUAACACAUGCAAGUCGAACGUUGUUC UAAAAAAAAAAUUUUUUUUAAGAGUUGAGAACAAAGUGGCGAACGGGUGAGUAAGGCGUGGGAAUCUGCCGAACAGUUCG GGCUAAAUCCCGAAGAAAGCUAGAAAGCGCUGUUUGAUGAGCCUGCGUAGUAUUAGGUAGUUGGUUAGGUAAAGGCUGAC CAAGCCGAUGAUGCUUAGCUGGUCUUUUCGGACGAUCAGCCACACCGGGACUGAGACACGGCCCGGACUCCCACGGGGGG CAGCAGUGGGGAAUCUUGGACAAUGGGCGCAAGCCUGAUCCAGCAAUAUUGCGUGAGUGAAGAAGGGCAAUUCCGCUCGU AAAGCUCUUUCGUCGAGUGCGCGAUCAUGACAUGACUCGAGGAAGAAGCCCCGGCUAACUCCGUGCCAGCAGCC(G7M)C GGUAAAACGGGGGGGGCAAGUGUUCUUCGGAAUGACUAGGCGUAAAGGGCACGUAGGCGGUUCAUCGGGUUGAAAGUGAA AGUCGUCAAAAAGUGGCGGAAUGCUUUCGAAACCACUGAACUUGAGUGAGACAGAGGAGAGUGGAAUUUCGUGUGGAGGG GUGAAAUCUACGGAUCUACGAAGGAACGCCAAAAGCGAAGGCAGCUCUCUGGGUCUCUACCGACGCUGGUGUGCGAAAGC AUGGGGAGCGAACGGGAUUAGAUACCCUGGUAGUCCAUGCCGUAAACGAUGAGUGUUCGCCCUUGGUCUAUUCUGAUCUG GGGCUCAGCUAACGCGUUAAACACUCCGCCUGGGGAGUACGGUCGCAAGACCAAAACUCAAAGGAAUUGACGGGGGCCUG CACAAGCGGUGGAGCAUGUGGUUUAAUUCGAUACAACGCGCAGAACCUUACCAGCCCUUGACAUAUGAACGAGAAACCCU AUCCUUAACGGGAUGGUACUGACUUUCAUACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUCGUGAGAUGUUUGGUUAAG UCCUAUAACGAGCGAAACCCUCGCCUUGUGUUGCUCAGACAUGCGCCUAAGGAGAACUCCGAAACAAACAAAAAGGUGCG UGACGCACUCACGAGGGACUGCCAGUGAUAUACUGGAGGAAGGUGGGGAUGACGUCAAGUCCGCAUGGCCCUUAUGGGCU GGGCCACACACGUGCUACAAUGGCAAUUACAAUGGGAAGCAAGGCUGUAAGGCGGAGCGAAUCCGGAAAGAUUGCCUCAG UUCGGAUUGUUCUCUGCAACUCGGGAACAUGAAGUUGGAAUCGCUAGUAAUCGCGGAUCAGCAUGCCGCGGUGAAUAUGU ACCCGGGCCCUGUACACACCG(4OC)CCGUCACACCCUGGGAAUUGGUUUCGCCCGAAGCAUCGGACCAAUGAUCACCCA GCACUUCUGUGUAACACUAGUCACACAAAGACUUUUGGUGGUCUUAUUGACGCAUACCACGGUGGGGUCUUCGACUGGGG UGAAGUCG(UR3)AACAAGGUAGCCGUAGG(2MG)G(MA6)(MA6)CCUGUGGCUGGAUUAUCC POTASSIUM ION 3.9098e-05 10 K MAGNESIUM ION 2.4305e-05 85 MG ADENOSINE-5'-TRIPHOSPHATE 0.000507181 1 ATP ZINC ION 6.5409e-05 1 ZN GUANOSINE-5'-TRIPHOSPHATE 0.0005231799999999999 1 GTP singleParticle particle 3 7.5 Quantifoil R2/1 COPPER 300 CARBON HOLEY GLOW DISCHARGE 20 ETHANE 100 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.5 2.5 165000.0 TFS FALCON 4i (4k x 4k) 50.96 1 931475 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION 2.27 FSC 0.143 CUT-OFF cryoSPARC 208855 MAXIMUM LIKELIHOOD cryoSPARC MAXIMUM LIKELIHOOD cryoSPARC cryoSPARC emd_50014.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 588 588 588 0 0 0 588 588 588 524.87823 524.87823 524.87823 90.0 90.0 90.0 X Y Z -0.5723365 1.0586492 -0.00063157093 0.023356847 0.89265007 0.89265007 0.89265007 0.0377 AUTHOR ::::EMDATABANK.org::::EMD-50014:::: REAL emd_50014_msk_1.map emd_50014_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 588 588 588 0 0 0 588 588 588 524.87823 524.87823 524.87823 90.0 90.0 90.0 X Y Z -0.43251908 0.679067 0.000025755244 0.049714066 0.89265007 0.89265007 0.89265007 AUTHOR ::::EMDATABANK.org::::EMD-50014:::: emd_50014_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 588 588 588 0 0 0 588 588 588 524.87823 524.87823 524.87823 90.0 90.0 90.0 X Y Z -0.41630587 0.74081016 0.000028754612 0.049936004 0.89265007 0.89265007 0.89265007 AUTHOR ::::EMDATABANK.org::::EMD-50014::::