2026-01-28 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_ISSN _citation.journal_volume _citation.pdbx_database_id_DOI _citation.year _citation_author.identifier_ORCID _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_ISSN _citation.journal_volume _citation.pdbx_database_id_DOI _citation.year _citation_author.identifier_ORCID _em_admin.last_update DATABASE_REFERENCES citation citation_author em_admin _citation.page_first _citation.page_last _citation.pdbx_database_id_PubMed _citation.title _citation_author.identifier_ORCID _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.page_first _citation.page_last _citation.pdbx_database_id_PubMed _citation.title _citation_author.identifier_ORCID _em_admin.last_update PDBe PDBe 2024-08-06 2025-12-17 2025-12-17 2026-01-28 Ministerio de Ciencia e Innovacion (MCIN) PID2020-12141GB-100 Spain Generalitat de Catalunya 2021SGR00423 Spain Alcaide-Jimenez A Baudin F Canals A Machon C Murciano B Fabrega-Ferrer M Bantysh O Perez-Luque R Krukonis ES Muller CW Coll M RNA polymerase, Transcription Activation Complex, toxT, ToxR, TcpP, Vibrio cholerae, TRANSCRIPTION Alcaide-Jimenez A Canals A Baudin F Machon C Fabrega-Ferrer M Bantysh O Perez-Luque R Murciano B Mohammad AA Rowse MJ Ferracciolo JM Krukonis ES Muller CW Coll M Sci Adv US 12 eadx9680 eadx9680 2026 41533787 doi:10.1126/sciadv.adx9680 2375-2548 EMD-51955 consensus EM volume

Virulent transcription complex 4 consensus map

EMD-51956 focused EM volume

Virulent transcription complex 4 focused map 1

EMD-51958 focused EM volume

Virulent transcription complex 4 focused map 2

9gds FULLOVERLAP EMDB EMD-51277 associated EM volume

Virulent transcription complex #5

EMDB EMD-51955 consensus EM volume

Virulent transcription complex 4 consensus map

EMDB EMD-51956 focused EM volume

Virulent transcription complex 4 focused map 1

EMDB EMD-51958 focused EM volume

Virulent transcription complex 4 focused map 2

Virulent transcription complex #5 Virulent transcription complex #5 0 1 2 3 4 5 6 7 8 9 DNA-directed RNA polymerase 1 2 3 Vibrio cholerae O395 Double stranded DNA 1 1 4 5 6 7 8 9 Vibrio cholerae O395 Toxin co-regulated pilus biosynthesis protein P Vibrio cholerae O395 0.013447507 2 Escherichia coli BL21(DE3) LEVO MGYVRVIYQFPDNLWWNEASNQVYYAQDPMKPERLIGTPSIMQAKLLKILCEYHPSPCPNDQIIKALWPHGFISSESLTQ AIKRTRDFLNDEHKTLIENVKLQGYRINIIQVIVS A0A0H3AHV1 toxT promoter non-template DNA strand Vibrio cholerae O395 0.033983851999999995 1 (DT)(DC)(DA)(DA)(DA)(DA)(DA)(DA)(DC)(DA)(DT)(DA)(DA)(DA)(DA)(DT)(DA)(DA)(DC)(DA) (DT)(DG)(DA)(DG)(DT)(DT)(DA)(DC)(DT)(DT)(DT)(DA)(DT)(DG)(DT)(DT)(DT)(DT)(DT)(DC) (DT)(DT)(DA)(DT)(DG)(DT)(DA)(DA)(DT)(DA)(DC)(DG)(DT)(DC)(DT)(DG)(DT)(DA)(DA)(DC) (DT)(DT)(DG)(DT)(DT)(DC)(DT)(DT)(DA)(DT)(DG)(DT)(DC)(DT)(DG)(DT)(DT)(DA)(DT)(DG) (DT)(DA)(DG)(DT)(DT)(DA)(DA)(DT)(DA)(DT)(DA)(DA)(DT)(DT)(DG)(DA)(DA)(DG)(DG)(DA) (DT)(DT)(DT)(DT)(DA)(DA)(DG)(DA)(DT)(DA) DNA toxT promoter template DNA strand Vibrio cholerae O395 0.033971957000000004 1 (DT)(DA)(DT)(DC)(DT)(DT)(DA)(DA)(DA)(DA)(DT)(DC)(DG)(DA)(DA)(DG)(DT)(DT)(DA)(DA) (DT)(DA)(DT)(DA)(DA)(DA)(DA)(DC)(DT)(DA)(DC)(DA)(DT)(DA)(DA)(DC)(DA)(DG)(DA)(DC) (DA)(DT)(DA)(DA)(DG)(DA)(DA)(DC)(DA)(DA)(DG)(DT)(DT)(DA)(DC)(DA)(DG)(DA)(DC)(DG) (DT)(DA)(DT)(DT)(DA)(DC)(DA)(DT)(DA)(DA)(DG)(DA)(DA)(DA)(DA)(DA)(DC)(DA)(DT)(DA) (DA)(DA)(DG)(DT)(DA)(DA)(DC)(DT)(DC)(DA)(DT)(DG)(DT)(DT)(DA)(DT)(DT)(DT)(DT)(DA) (DT)(DG)(DT)(DT)(DT)(DT)(DT)(DT)(DG)(DA) CP000627.1 DNA DNA-directed RNA polymerase subunit beta Vibrio cholerae O395 0.149696531 1 Escherichia coli BL21(DE3) LEVO MVYSYTEKKRIRKDFGTRPQVLDIPYLLSIQLDSFEKFIEQDPEGQYGLEAAFRSVFPIQSYNGNSELQYVSYRLGEPVF DVKECQIRGVTYSKPLRVKLRLVIFDKDAPAGTVKDIKEQEVYMGEIPLMTENGTFVINGTERVIVSQLHRSPGVFFDSD KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLYVRIDRRRKLPASIILRVLGKTSAEILDIFFEKVNFEVKDQTLMME LVPERLRGETATFDIEADGKVYVEKGRRVTARHIRQLEKDGVNFIEVPVEYIVGKVSAKDYVNEATGELIITANQEISLE ALANLSQAGYKKLEVLFTNDLDHGPFMSETLRVDSTTDRISALVEIYRMMRPGEPPTKEAAESLFESLFFSAERYDLSTV GRMKFNSSIGREDAEEQGTLDEVDIIEVMKKLISIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLS LGDLDNVMPQDLINAKPISAAVKEFFGSSQLSQFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHVTHYGRLCP IETPEGPNIGLINSLSAFARCNEYGFLETPYRRVVNGVVTDEVDYLSAIEEGQFVIAQANAKLTEEGGFADELVTARQKG ESGLHPREHVDYMDVATNQVVSIAASLIPFLEHDDANRALMGANMQRQAVPTLRSEKPLVGTGIERNVAVDSGVTAVAKR GGVIQSVDASRIVVKVNEEELIPGEAGIDIYNLTKYTRSNQNTCINQRPCVMPGEPVARGDVLADGPSTDLGELALGQNM RIAFMPWNGYNFEDSILVSERVVQDDRFTTIHIQELSCVARDTKLGAEEITADIPNVGEAALSKLDESGIVYIGAEVKGG DILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDTSLRVPNSVAGTVIDVQVFTRDGVEKDKRALEIEQMQLKEAKKDL TEEFQILEGGLLARVRSVLLAGGYTEAKLSSIERKKWLEQTLENEELQNQLEQLAEQYDELKADFDKKFEAKRRKITQGD DLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPVEDMPYDENGQPVDIVLNPLGVPSRMNIGQILEVHLGLA AKGIGDKINQMIKEQQELAKLREFLQKVYDLGDTRQRVDISELSDEDVRTLAHNLRAGLPVATPVFDGAPESSIKAMLEL ADLPASGQLTLFDGRTGDAFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAY GAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHAMEPGMPESFNVLLKEIRSLGINIELEDE A5F3P5 2.7.7.6 DNA-directed RNA polymerase subunit beta' Vibrio cholerae O395 0.15523428099999997 1 Escherichia coli BL21(DE3) LEVO MKDLLNFLKAQHKTEEFDAIKIGLASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKH RGVICEKCGVEVTQTKVRRDRMGHIELASPVAHIWFLKSLPSRIGLLMDMPLRDIERVLYFEMYVVTEPGMTDLERGQML TEEEYLDRLEEWGDEFTAKMGAEAIKDLLASMDLPAEAEQMREELDTTNSETKRKKLTKRLKLVEAFVASGNKPEWMILT VLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLELAAPDIIVRNEKRMLQESVDALLDNGRRGRAITGSN KRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYSKLETRGLATTIKAAKKM VEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNADFDGDQMAVHVPLTLEAQLEA RTLMMSTNNILSPASGDPIIVPSQDVVLGLYYMTREKINAKGEGMYLTGPAEAEKAYRTKTAELHARVKVRITETIKHEN GKLTTETKMIDTTVGRAMLWQIVPKGLPYSLVNQKLGKKQISNLLNEAYRKLGLKDTVIFADQIMYTGFAYAALSGVSVG IDDMVVPAAKYTEIAEAEEEVREIQEQFQSGLVTAGERYNKVIDIWASTNDRVAKAMMENLSSEQVINRQGEQEKQESFN SIYMMADSGARGSAAQIRQLAGMRGLMARPDGSIIETPITANFKEGLNVLQYFISTHGARKGLADTALKTANSGYLTRRL VDVAQDVVVTEHDCGTLEGVVMTPHIEGGDVKVALTELALGRVVSEDILKPGTDEVLIPRNTLLDEKWCKVINDNSVDQI KVRSVVTCDSDFGCCAQCYGRDLARGHLVNQGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASTAAAENSIQAKNNGSVKL HNAKFVTNKDGKLVITSRASELTIIDEFGRTKEKHKLPYGSMLSKADGDAVAAGETVANWEAHTMPIITEVAGRVQFVDM IDGVTVSRQTDDLTGLSSSEVTEAAARPAAGKDMRPAIKLVDANGKDVLIPGTDMPAQYFLPGKAIVNLDDGAEVNVGDT LARIPQKSGGNKDITGGLPRVADLFEARKPKEPAILAEHSGTVSFGKETKGKRRLIITRDSGDTYEEMIPKHRQLNVFEG ERIERGDVIADGPESPHDILRLRGIHAVTTYIANEVQEVYRLQGVKINDKHIETIVRQMLRKCTITFAGDSEFLPGETVE YSQVKIANRKLVEEGKEPARFERELLGITKASLATESFISAASFQETTRVLTEAAVSGKRDDLRGLKENVIVGRLIPAGT GFAYHQDRQAKRAQEQQGPSAEQATDNLAALLNAGFSSDDE A5F3P6 2.7.7.6 DNA-directed RNA polymerase subunit omega Vibrio cholerae O395 0.010068355 1 Escherichia coli BL21(DE3) LEVO MARVTVQDAVEKIGNRFDLVLVAARRARQMQSGGKDALVPEENDKPTVIALREIEEGLITKDVLDARERQEQQEQEAAEL AAVSSIMHNR A5F4S8 2.7.7.6 DNA-directed RNA polymerase subunit alpha Vibrio cholerae O395 0.036461472999999994 3 Escherichia coli BL21(DE3) LEVO MQGSVTEFLKPRLVDIEQISTTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIEGVLHEYSTKEGVQEDILE ILLNLKGLAVRVAEGKDEVFITLNKSGSGPVVAGDITHDGDVEIVNPEHVICHLTSDNAAIAMRIKVERGRGYVPASARI HTEEDERPIGRLLVDATFSPVDKIAYSVEAARVEQRTDLDKLVIDMETNGTLEPEEAIRRAATILAEQLDAFVDLRDVRV PEEKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLE NWPPASIAED A5F572 2.7.7.6 RNA polymerase sigma factor RpoD Vibrio cholerae O395 0.070749648 1 Escherichia coli BL21(DE3) LEVO MDQNPQSQLKQLVLRGKEQGYLTYAEVNDHLPAEIVDSEQVEDIIQMINDMGIKVVETAPDADDLALSDDTTITDEDAAE AAAAALSSVESEIGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGINQVQSAIAEYPGTIPYILEQFDRVQAEELRL TDLISGFVDPNDMETEAPTATHIGSELSEADLADEDDAVVEDEDEDGDGESSDSEEEVGIDPELAREKFNELRGKFQNLQ LAVNEFGRDSHQASEASDLVLDIFREFRLTPKQFDHLVETLRTSMDRVRTQERLVMKAVVEVAKMPKKSFIALFTGNESN EEWLDKVLASDKPYVAKVREQEEEIRRSIQKLQMIEQETSLSVERIKDISHRMSIGEAKARRAKKEMVEANLRLVISIAK KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQ EMGREPLPEELAERMQMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATATSLKAATRDVLAGLT PREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDE A0A0H3AJ64 Cholera toxin transcriptional activator Vibrio cholerae O1 biovar El Tor str. N16961 0.012832575 1 Escherichia coli BL21(DE3) LEVO MKFILAEKFTFDPLSNTLIDKEDSEEIIRLGSNESRILWLLAQRPNEVISRNDLHDFVWREQGFEVDDSSLTQAISTLRK MLKDSTKSPQYVKTVPKRGYQLIARVETVE P15795 MAGNESIUM ION 2.4305e-05 1 MG ZINC ION 6.5409e-05 2 ZN singleParticle particle 0.4 8.0

10 mM HEPES pH 8.0, 150 mM NaCl, 1 mM DTT and 0.1 mM EDTA

ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.7000000000000001 1.8 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON IV (4k x 4k) 50.71 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 2.9 FSC 0.143 CUT-OFF 88323 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD