2025-10-01 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2024-09-20 2025-10-01 2025-10-01 2025-10-01 Volkswagen Foundation 93090 Germany German Federal Ministry for Education and Research 03Z22EN12 Germany Cryo-EM tomogram of JCVI-Syn3A cells grown on a defined lipid diets Justice IJ Saenz JS Minimal cell, cell membranes, cell phenotype, defined diet, JCVI-Syn3A, UNKNOWN FUNCTION Justice IJ Saenz JS Cryo-EM tomograms of JCVI-Syn3A Cells on various defined diets To Be Published 0353 EMDB EMD-51614 associated EM volume JCVI-Syn3A JCVI-Syn3A 0 JCVI-Syn3A tomography cell 7.0 20.0 C8H18N2O4S HEPES 200.0 NaCl Sodium Chloride
pH 7
 Quantifoil COPPER 200 CARBON HOLEY 100.0 GLOW DISCHARGE AIR
Cells were disperse in buffer
Nano Biosols 10 NO SECTIONING FEI TITAN FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 3.0 6.0 30000.0 NITROGEN GATAN K2 SUMMIT (4k x 4k) 0.15 90.0 1 eTomo 441 eTomo PHASE FLIPPING ONLY emd_51614.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 1541 1508 441 0 0 0 1541 1508 441 14978.5205 14657.761 4286.52 90.0 90.0 90.0 X Y Z -13.160076999999999 4.2500887 -0.5110331 0.28655815 9.72 9.72 9.72 AUTHOR A Cryo-EM tomogram of a JCVI-Syn3A cell on a Diether PC Cholesterol diet displaying an Internal Membrane phenotype