2026-01-28 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2024-10-10 2025-12-17 2025-12-17 2026-01-28 Ministerio de Ciencia e Innovacion (MCIN) PID2020-12141GB-100 Spain Generalitat de Catalunya 2021SGR00423 Spain Focused map #2 of the cryo-EM structure of Vibrio cholerae RNA polymerase Transcription Activation Complex with ToxR transcription factor and bound to an ompU promoter DNA fragment Alcaide-Jimenez A Baudin F Canals A Machon C Murciano B Fabrega-Ferrer M Bantysh O Perez-Luque R Krukonis ES Muller CW Coll M RNA polymerase, Open promoter complex, ompU, Vibrio cholerae, TRANSCRIPTION Alcaide-Jimenez A Canals A Baudin F Machon C Fabrega-Ferrer M Bantysh O Perez-Luque R Murciano B Mohammad AA Rowse MJ Ferracciolo JM Krukonis ES Muller CW Coll M Structures of Vibrio cholerae transcription complexes reveal how ToxR and TcpP recruit the RNA polymerase and activate virulence genes. Sci Adv US 12 eadx9680 eadx9680 2026 41533787 doi:10.1126/sciadv.adx9680 2375-2548 EMDB EMD-51776 associated EM volume Virulent transcription complex #3 Virulent transcription complex #3 0 1 2 3 4 5 6 7 8 DNA-directed RNA polymerase 1 1 2 3 4 5 6 Vibrio cholerae O395 Double stranded DNA 1 7 8 Vibrio cholerae O395 singleParticle particle 0.4 8.0
10 mM HEPES pH 8.0, 500 mM NaCl, 1 mM DTT and 0.1 mM EDTA
 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.7000000000000001 2.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K3 BIOQUANTUM (6k x 4k) 51.24 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 3.8 FSC 0.143 CUT-OFF 158389 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_51776.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 450 450 450 0 0 0 450 450 450 369.90002 369.90002 369.90002 90.0 90.0 90.0 X Y Z -0.10177215 2.1578736 0.00008323828 0.013048169 0.822 0.822 0.822 0.101 AUTHOR TAC_ompU Multibody Refinement Map C Sharpened. emd_51776_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 450 450 450 0 0 0 450 450 450 369.90002 369.90002 369.90002 90.0 90.0 90.0 X Y Z -0.0056080185 0.016993914 -0.00016540634 0.00034831665 0.822 0.822 0.822 AUTHOR TAC_ompU Multibody Refinement Map C unsharpened. emd_51776_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 450 450 450 0 0 0 450 450 450 369.90002 369.90002 369.90002 90.0 90.0 90.0 X Y Z -0.021383142 0.024099695 -0.00016697445 0.0010282479 0.822 0.822 0.822 AUTHOR TAC_ompU Multibody Refinement Map C unfiltered half map 1. emd_51776_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 450 450 450 0 0 0 450 450 450 369.90002 369.90002 369.90002 90.0 90.0 90.0 X Y Z -0.015146518 0.025660934 -0.00016389617 0.0010225321 0.822 0.822 0.822 AUTHOR TAC_ompU Multibody Refinement Map C unfiltered half map 2.