2026-03-18 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update DATABASE_REFERENCES citation em_admin _citation.journal_volume _citation.page_first _citation.page_last _em_admin.last_update EXPERIMENTAL_SUMMARY citation em_admin _citation.journal_volume _citation.page_first _citation.page_last _em_admin.last_update PDBe PDBe 2024-10-11 2026-02-18 2026-02-18 2026-03-18 Research Council of Lithuania S-MIP-19-32 Lithuania Cas9:crRNA:tracrRNA in complex with PAM-containing non-cognate DNA, PAM-bound conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system Sasnauskas G Gaizauskaite U Tamulaitiene G Cas9, PAM, CRISPR-Cas, RNA BINDING PROTEIN, DNA BINDING PROTEIN Gaizauskaite U Tamulaitiene G Silanskas A Gasiunas G Siksnys V Sasnauskas G Structural insights into Cas9-mediated prespacer selection in CRISPR-Cas adaptation. Mol.Cell US 86 791 804.e9 2026 41702403 doi:10.1016/j.molcel.2026.01.022 1097-2765 2168 MOCEFL 9h2g FULLOVERLAP EMDB EMD-51801 associated EM volume
Cas9:crRNA:tracrRNA in complex with PAM-containing non-cognate DNA, PAM-bound conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system
 Cas9:crRNA:tracrRNA bound to PAM-containing duplex DNA Cas9:crRNA:tracrRNA bound to PAM-containing duplex DNA 0 1 2 3 4 5 Streptococcus thermophilus DGCC 7710 CRISPR-associated endonuclease Cas9 Streptococcus thermophilus DGCC 7710 0.16249853099999997 1 Escherichia coli BL21(DE3) LEVO MTKPYSIGLDIGTNSVGWAVITDNYKVPSKKMKVLGNTSKKYIKKNLLGVLLFDSGITAEGRRLKRTARRRYTRRRNRIL YLQEIFSTEMATLDDAFFQRLDDSFLVPDDKRDSKYPIFGNLVEEKVYHDEFPTIYHLRKYLADSTKKADLRLVYLALAH MIKYRGHFLIEGEFNSKNNDIQKNFQDFLDTYNAIFESDLSLENSKQLEEIVKDKISKLEKKDRILKLFPGEKNSGIFSE FLKLIVGNQADFRKCFNLDEKASLHFSKESYDEDLETLLGYIGDDYSDVFLKAKKLYDAILLSGFLTVTDNETEAPLSSA MIKRYNEHKEDLALLKEYIRNISLKTYNEVFKDDTKNGYAGYIDGKTNQEDFYVYLKNLLAEFEGADYFLEKIDREDFLR KQRTFDNGSIPYQIHLQEMRAILDKQAKFYPFLAKNKERIEKILTFRIPYYVGPLARGNSDFAWSIRKRNEKITPWNFED VIDKESSAEAFINRMTSFDLYLPEEKVLPKHSLLYETFNVYNELTKVRFIAESMRDYQFLDSKQKKDIVRLYFKDKRKVT DKDIIEYLHAIYGYDGIELKGIEKQFNSSLSTYHDLLNIINDKEFLDDSSNEAIIEEIIHTLTIFEDREMIKQRLSKFEN IFDKSVLKKLSRRHYTGWGKLSAKLINGIRDEKSGNTILDYLIDDGISNRNFMQLIHDDALSFKKKIQKAQIIGDEDKGN IKEVVKSLPGSPAIKKGILQSIKIVDELVKVMGGRKPESIVVEMARENQYTNQGKSNSQQRLKRLEKSLKELGSKILKEN IPAKLSKIDNNALQNDRLYLYYLQNGKDMYTGDDLDIDRLSNYDIDHIIPQAFLKDNSIDNKVLVSSASNRGKSDDFPSL EVVKKRKTFWYQLLKSKLISQRKFDNLTKAERGGLLPEDKAGFIQRQLVETRQITKHVARLLDEKFNNKKDENNRAVRTV KIITLKSTLVSQFRKDFELYKVREINDFHHAHDAYLNAVIASALLKKYPKLEPEFVYGDYPKYNSFRERKSATEKVYFYS NIMNIFKKSISLADGRVIERPLIEVNEETGESVWNKESDLATVRRVLSYPQVNVVKKVEEQNHGLDRGKPKGLFNANLSS KPKPNSNENLVGAKEYLDPKKYGGYAGISNSFAVLVKGTIEKGAKKKITNVLEFQGISILDRINYRKDKLNFLLEKGYKD IELIIELPKYSLFELSDGSRRMLASILSTNNKRGEIHKGNQIFLSQKFVKLLYHAKRISNTINENHRKYVENHKKEFEEL FYYILEFNENYVGAKKNGKLLNSAFQSWQNHSIDELCSSFIGPTGSERKGLFELTSRGSAADFEFLGVKIPRYRDYTPSS LLKDATLIHQSVTGLYETRIDLAKLGEGLEGHHHHHH G3ECR1 3.1.-.- crRNA (26-MER) synthetic construct 0.013609083 1 CGCUAAAGAGGAAGAGGACAGUUUUAGAGCUGUGUUGUUUCG tracrRNA (65-MER) synthetic construct 0.024119356999999998 1 CGAAACAACACAGCGAGUUAAAAUAAGGCUUAGUCCGUACUCAACUUGAAAAGGUGGCACCGAUUCGGUGUUUUU DNA synthetic construct 0.015003586 1 (DG)(DA)(DT)(DG)(DT)(DC)(DT)(DT)(DC)(DC)(DA)(DA)(DT)(DT)(DT)(DA)(DC)(DT)(DA)(DC) (DT)(DC)(DG)(DT)(DT)(DC)(DT)(DG)(DG)(DT)(DG)(DT)(DT)(DT)(DC)(DT)(DC)(DG)(DA)(DG) (DG)(DC)(DG)(DT)(DC)(DA)(DG)(DC)(DC) DNA DNA synthetic construct 0.015182787 1 (DG)(DG)(DC)(DT)(DG)(DA)(DC)(DG)(DC)(DC)(DT)(DC)(DG)(DA)(DG)(DA)(DA)(DA)(DC)(DA) (DC)(DC)(DA)(DG)(DA)(DA)(DC)(DG)(DA)(DG)(DT)(DA)(DG)(DT)(DA)(DA)(DA)(DT)(DT)(DG) (DG)(DA)(DA)(DG)(DA)(DC)(DA)(DT)(DC) DNA CALCIUM ION 4.0078e-05 1 CA singleParticle particle 7.5 Quantifoil R1.2/1.3 COPPER 300 CARBON ETHANE 95 277 FEI VITROBOT MARK IV TFS GLACIOS OTHER OTHER FIELD EMISSION GUN 200 50.0 2.7 1.0 2.0 92000.0 NITROGEN FEI FALCON III (4k x 4k) COUNTING 1 3404 46.33 30.0 1 3199891
blob picking in cryoSPARC live session
 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION ab-initio cryoSPARC C1 FOURIER SPACE 3.28 FSC 0.143 CUT-OFF cryoSPARC 4.6.2 41668 MAXIMUM LIKELIHOOD cryoSPARC OTHER emd_51801.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 220.0 220.0 220.0 90.0 90.0 90.0 Z Y X -22.116648000000001 31.185745000000001 0.000000000002863 1.0 1.1 1.1 1.1 3.5 AUTHOR sharpened map (phenix.auto_sharpen) AlphaFold in silico model emd_51801_msk_1.map emd_51801_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 220.0 220.0 220.0 90.0 90.0 90.0 X Y Z -0.1381658 0.4066247 0.00045289844 0.01999707 1.1 1.1 1.1 AUTHOR Unsharpened map, input to phenix.auto_sharpen (3.10 A, b_iso_to_d_cut) emd_51801_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 220.0 220.0 220.0 90.0 90.0 90.0 X Y Z -0.12593953 0.45208037 -0.00073570415 0.020682938 1.1 1.1 1.1 AUTHOR half map 2 emd_51801_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 0 0 0 200 200 200 220.0 220.0 220.0 90.0 90.0 90.0 X Y Z -0.18388179 0.457159 -0.00070827355 0.030656891 1.1 1.1 1.1 AUTHOR half map 1