2025-08-13 REL PDBe PDBe PDBe 2024-10-28 2025-08-13 2025-08-13 2025-08-13 Biotechnology and Biological Sciences Research Council (BBSRC) BB/X007251/1 United Kingdom KtrA.ADP with a 54ms plunge time on the chameleon. Hirst IJ Muench SP Darrow MC Scarff CA Thompson RF sample preparation, air-water interface, potassium transport, RCK C domain, TRANSPORT PROTEIN Hirst IJ Chiang WT Hu NJ Scarff CA Thompson RF Darrow MC Muench SP Untangling the effects of flexibility and the AWI in cryoEM sample preparation: A case study using KtrA. J.Struct.Biol. US 217 108206 108206 2025 40324569 doi:10.1016/j.jsb.2025.108206 1095-8657 0803 JSBIEM EMDB EMD-51920 associated EM volume KtrA, the regulatory subunit from the KtrAB potassium transporter KtrA, the regulatory subunit from the KtrAB potassium transporter 0
This is map is part of an investigation into the effects of sample preparation on map quality.
Bascillus subtilis 0.198 singleParticle particle 8 8.0 150.0 KCl potassium chloride 50.0 Tris tris
50 mM Tris-HCl, 150 mM KCl, 0.5 mM TCEP
 ETHANE 100 277 SPOTITON
The commercialised version of the spotiton, the chameleon was used with a plunge time of 54 ms. In-house self wicking grids from SPT Labtech were used..
TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 1.5 3.0 96000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON IV (4k x 4k) 1 11631 7.17 49.8 1 383653 RELION 4.0 CTFFIND4 was used in relion for CTF estimation PHASE FLIPPING AND AMPLITUDE CORRECTION 5 ab initio models
ab initio job in cryoSPARC with 5 classes
 8.0 FSC 0.143 CUT-OFF cryoSPARC 35383 MAXIMUM LIKELIHOOD cryoSPARC
Non-uniform refinement
 MAXIMUM LIKELIHOOD cryoSPARC
Non-uniform refinement
 emd_51920.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 416 416 416 0 0 0 416 416 416 357.76 357.76 357.76 90.0 90.0 90.0 X Y Z -0.10726522 0.43063253 0.00048367868 0.018093128 0.86 0.86 0.86 0.14 AUTHOR The non-sharpened map of the 54ms plunge time chameleon dataset. This is the map used for comparison with the other datasets. emd_51920_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 416 416 416 0 0 0 416 416 416 357.76 357.76 357.76 90.0 90.0 90.0 X Y Z -0.84444916 1.1932834 0.0004836787 0.029144326 0.86 0.86 0.86 AUTHOR The sharpened map of the 54ms plunge time chameleon dataset. Not used for comparison. emd_51920_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 416 416 416 0 0 0 416 416 416 357.76 357.76 357.76 90.0 90.0 90.0 X Y Z -0.23746999 0.50232476 0.00073375495 0.02349749 0.86 0.86 0.86 AUTHOR emd_51920_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 416 416 416 0 0 0 416 416 416 357.76 357.76 357.76 90.0 90.0 90.0 X Y Z -0.22999834 0.4982137 0.000754663 0.02349398 0.86 0.86 0.86 AUTHOR