2025-09-17 REL PDBe PDBe PDBe 2024-11-05 2025-09-17 2025-09-17 2025-09-17 Agence Nationale de la Recherche (ANR) ANR-19-CE11-0014 France Agence Nationale de la Recherche (ANR) ANR-22-CE44-0021 France H2020 Marie Curie Actions of the European Commission 101063049_3D-V2R European Union Focused refinement Fab-Nb (cryo-EM structure of inactive human arginine-vasopressin (AVP) V2 receptor (V2R) with Mambaquaretin1 K39A (MQK39A)) Bous J Fouillen A Couvineau P Orcel H Mary C Mendre C Schulte G Granier S Gilles N Mouillac B GPCR, V2R, TVP, tolvaptan, MEMBRANE PROTEIN Fouillen A Bous J Couvineau P Orcel H Mary C Lafleur L Pierre T Mendre C Gilles N Schulte G Granier S Mouillac B Inactive structures of the vasopressin V2 receptor reveal distinct binding modes for Tolvaptan and Mambaquaretin toxin. Nat Commun UK 16 3899 3899 2025 40274867 doi:10.1038/s41467-025-59114-5 2041-1723 EMDB EMD-52010 associated EM volume Inactive V2R-bril bound to tolvaptan stabillized with anti-BRIL Fab and anti-BRIL Fab Nanobody Inactive V2R-bril bound to tolvaptan stabillized with anti-BRIL Fab and anti-BRIL Fab Nanobody 0 1 2 3 4 Homo sapiens 0.115 singleParticle particle 22 7.5 Quantifoil R0.6/1 GOLD CARBON HOLEY ETHANE 100 278 LEICA EM GP TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.6 1.8 GATAN K3 (6k x 4k) 80.0 1 cryoSPARC 4.5.1 PHASE FLIPPING AND AMPLITUDE CORRECTION 2.5 FSC 0.143 CUT-OFF cryoSPARC 4.5.1 340370 MAXIMUM LIKELIHOOD cryoSPARC 4.5.1 MAXIMUM LIKELIHOOD cryoSPARC 4.5.1 RELION 4 emd_52010.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 304.56 304.56 304.56 90.0 90.0 90.0 X Y Z -0.9698622 2.0699167 -0.00044281108 0.025291093 1.0152 1.0152 1.0152 0.238 AUTHOR AlphaFold in silico model FLEXIBLE FIT REAL emd_52010_msk_1.map emd_52010_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 304.56 304.56 304.56 90.0 90.0 90.0 X Y Z -0.22037524 0.8109937 -0.00044281106 0.01112924 1.0152 1.0152 1.0152 AUTHOR emd_52010_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 304.56 304.56 304.56 90.0 90.0 90.0 X Y Z -0.26780027 0.94327855 0.000042289004 0.025314188 1.0152 1.0152 1.0152 AUTHOR emd_52010_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 304.56 304.56 304.56 90.0 90.0 90.0 X Y Z -0.27923244 0.9763331 0.000051481966 0.025253652 1.0152 1.0152 1.0152 AUTHOR