2026-01-21 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2024-11-07 2026-01-21 2026-01-21 2026-01-21 Swiss National Science Foundation CRSII5_183481 Switzerland Cryo-EM consensus map of the canine distemper virus tetrameric attachment H glycoprotein in complex with two different Nanobodies Djabeur N Jeckelmann JM Fotiadis D canine distemper virus, hemagglutinine, CDV, H-protein, Complex, Nanobody, VIRAL PROTEIN Scherer M Djabeur N Siering O Jeckelmann JM Wyss M Cresci M Di Palma Subran M Riedl R Chames P Pfaller CK Sawatsky B Fotiadis D Plattet P Protection against lethal canine distemper virus infection by a dual epitope-targeting synthetic antibody. Nat Commun UK 17 103 103 2026 41501035 doi:10.1038/s41467-025-67600-z 2041-1723 EMDB EMD-52020 associated EM volume Purified CDV-solH in complex with Nb H7 and Nb H9 Purified CDV-solH in complex with Nb H7 and Nb H9 0 1 2 3 4 5 6 7 8 9 10 11 12 0.3731 Hemagglutinin glycoprotein 1 1 2 7 8 Morbillivirus canis A75/17 Nanobodies NbH7 and NbH9 1 3 4 5 6 9 10 11 12
Nanobodies NbH7 and NbH9 purified separatlely
Lama glama singleParticle particle 1.2 7.6 20.0 Tris-HCl Tris(hydroxymethyl)aminomethane hydrochloride 100.0 NaCl sodium chloride 0.25 C14H28O6 Octyl glycoside
20 mM Tris-HCl, 100 mM NaCl, 0.25% OG (w/v)
 ETHANE 100 277.15 FEI VITROBOT MARK IV
Blot Time: 4.5 sec Blot Force: -4 Drain Time: 0 Wait time: 0.
Purified CDV-solH in complex with Nb H7 and Nb H9 in a molar ratio = 1/2.5/2.5
 TFS KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.8 2.5 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN TFS Selectris 20 FEI FALCON IV (4k x 4k) 9162 30.0 1 304110 Gctf 1.6 cryoSPARC 4 PHASE FLIPPING AND AMPLITUDE CORRECTION 7ZNY 3 C1 FOURIER SPACE 4.3 FSC 0.143 CUT-OFF cryoSPARC 4.2 118721 ANGULAR RECONSTITUTION RELION 4 ANGULAR RECONSTITUTION cryoSPARC 4.2 7 RELION 4 emd_52020.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 351.84 351.84 351.84 90.0 90.0 90.0 X Y Z -0.12917505 0.44385394 0.002145696 0.019051671 0.733 0.733 0.733 0.142 AUTHOR Consensus density map: receptor-binding tetrameric attachment (H)-protein of canine distemper virus (CDV) bound with 2 nanobodies, designated NbH7 and NbH9 7ZNY A PDB experimental model 7ZNY B PDB experimental model 7ZNY C PDB experimental model 7ZNY D PDB experimental model E AlphaFold in silico model F AlphaFold in silico model G AlphaFold in silico model H AlphaFold in silico model I AlphaFold in silico model J AlphaFold in silico model RIGID BODY FIT emd_52020_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 351.84 351.84 351.84 90.0 90.0 90.0 X Y Z -0.9349391 0.99111974 0.0015396589 0.11403461 0.733 0.733 0.733 AUTHOR Consensus half-density map A emd_52020_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 480 480 480 0 0 0 480 480 480 351.84 351.84 351.84 90.0 90.0 90.0 X Y Z -1.0028497 0.967609 0.0015668136 0.114049956 0.733 0.733 0.733 AUTHOR Consensus half-density map B