2025-11-19 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_CSD _citation.journal_id_ISSN _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update PDBe PDBe 2024-11-27 2025-08-27 2025-08-27 2025-11-19 Wellcome Trust 214222/Z/18/Z United Kingdom Bacteroides thetaiotaomicron BAM complex Silale A van den Berg B Outer membrane protein biogenesis, Beta-barrel assembly machinery, BAM, MEMBRANE PROTEIN Silale A Madej M Mikruta K Frey AM Hart AJ Basle A Scavenius C Enghild JJ Trost M Hirt RP van den Berg B Structure of a distinct beta-barrel assembly machinery complex in the Bacteroidota. Nat Microbiol UK 10 2845 2859 2025 41034344 doi:10.1038/s41564-025-02132-2 2058-5276 EMD-52200 focused EM volume EMD-52202 consensus EM volume 9hj3 FULLOVERLAP EMDB EMD-52200 focused EM volume EMDB EMD-52202 consensus EM volume EMDB EMD-52209 associated EM volume
Bacteroides thetaiotaomicron BAM complex
 Bacteroides thetaiotaomicron BAM complex Bacteroides thetaiotaomicron BAM complex 0 1 2 3 4 5 6 7 Bacteroides thetaiotaomicron VPI-5482 0.325 Outer membrane protein Bacteroides thetaiotaomicron VPI-5482 0.101527664 1 Bacteroides thetaiotaomicron VPI-5482 LEVO MHYRISFIFVTFICLFCFAATGFTQNTNTDEDSKPVILYSGTPKKYEIADIKVEGVKNYEDYVLIGLSGLSVGQTITVPG DEITGAIKRYWKHGLFSNVQITAEKIEGNKIWLKISLTQRPRIADVRYHGVKKSERTDFESKLGMVKGMQITPNTVDRAK TLIKRYFDDKGFKNAEVIIAQKDDPSNENQVIVDIDIDKKEKIKVHAIHITGNSAIKTSKLKKVMKKTNEKGKLLNLFRT KKFVPENFEADKQLIIDKYNELGYRDAMIVKDSVAQYDEKTVDVYMDIDEGQKYYLRNVTWVGNTLYPSEQLNFLLRMKK GDVYNQKLLGERTSTDDDAIGNLYYNNGYLFYNLDPVEVNIVGDSIDLEMRIYEGRQATINKINISGNDRLYENVVRREL RIRPGQLFSKDDLMRSLREIQQMGHFDPEKLQPDIQPDPVNGTVDIGLPLTSKANDQVEFSAGWGQTGIIGKLSLKFTNF SVANLLRPGENYRGILPQGDGQTLTISGQTNAKYYQSYSISFFDPWFGGKRPNSLSVSAFFSVQTDISSRYYNSSYFNNY YNSMYSGYGGYGMYNYGNYNNYENYYDPDKSIKMWGLSLGWGKRLKWPDDYFTLSAELAYQRYNLKDWQYFPVTNGKCND LSLSLTLARNSIDNPIFPRTGSDFSLSVQLTPPYSLFDGKDYKGYFYDPTDDRGITQDNMNKLHRWVEYHKWKFKAKTYT PLMDYIAHPKCLVLMTRTEFGLLGHYNKYKKSPFGTFDVGGDGMTGYSSYATESIALRGYENSSLTPYGKEGYAYARLGI ELRYPLMLETSTNIYVLGFLEAGNAWHDISKFNPFDLKRSAGIGVRIFLPMIGMMGIDWGYGFDKINGSKEYGGSQFHFI LGQEF Q8A1E1 Lipoprotein protein, putative Bacteroides thetaiotaomicron VPI-5482 0.031415555 1 LEVO MKKNIIITLLAAASLTSCGEYNKLLKSTDYEYKYEAAKNYFAKGQYNRSATLLNELITILKGTDKAEESLYMLGMSYYNQ KDYQTAAQTFITYFNTYPRGTFTELARFHAGKSLFLDTPEPRLDQSSTYQAIQQLQMFMEYFPNSTKKQEAQDMIFALQD KLVLKELYSAKLYYNLGNYLGNNYESCVITAQNALKDYPYTDYREELSILILRARHEMAIYSVEDKKMDRYRETIDEYYA FKNEFPESKYLKEAEKIFNESQKVIKD Q8AA92 Outer membrane protein Bacteroides thetaiotaomicron VPI-5482 0.047860691 1 LEVO MVGFKHTIWALLLMMVTGTAIAQNNTNSPYTRYGYGDLSDQSFGNSKAMGGIAFGLRDGAQINPTNPASYTAIDSLTFLF EGGVSLQNMNISGGGLKLNAKNASFDYLAMQFRLAPWMAMSVGLLPYSNVGYTVSDSQTTDNGLAYSRSFTGDGGLHQMY VGAGVKVLKNLSVGVNASYFWGDITRTRGMFYPGTSSYDSYQRKMVTSISDYKLDFGAQYTQALNKKSSLTIGAVYSPKH KLNNDYTSIVIMGASSSSYGTEYKDVLDATFELPNTFGVGFTYNYDKRLTVGADYSLQQWSKTNFGVVTSDENVRQDFNE TFTYCDRTKISVGAEYIPNLIGRSYFAHIKYRLGAYYTTPYYKIDGKKASREYGVTAGFGLPVPRSRSILSISGQFVRVK GLETNMVNENIFRVSIGLTFNERWFFKRRVE Q89ZL0 DUF6242 domain-containing protein Bacteroides thetaiotaomicron VPI-5482 0.055108051 1 LEVO MRIKFLSIIASFFMVSFVITSCLDNDNEVNYSPDATIRAFELDTIGYGVNYKFTIDQVSRLIYNVDSLPVNADTIINSIL IKTLTTASGIVTMKDQNDQDSIVNINDSIDLTKYVNATEKNNFLVLKVWAPNMEVQNEYKVNIRMHTMVPDSLSWGKDPI ANNPVRNTAEKQKVVTLGDKILLFAQNNEIYSTAIPAGSPTDRLNYGQKWDKETTGKLPDGADVTSIIRFVDKLYLLTKN KEVYNSNDGLTWTKDEVLNSDGVSVTNLITSFSDSDGSNHKKINGIAGIVEINGEKYFSFAEKDVTWEKDIDKLTVVPAE FPINNLSADVYATESGTLNAIVVGNTEDGLDNDTATVVWASEDGKAWIPMEIPSNNNCPKLVDPSIIHYNDAFYICGKET KDDAKGFQKFYTSPTLLVWKGVDRMFMLPGILPPVKLEGGVTQHPSLHESSFKGKEVNYTMVVDRNHYIWMVGGQGIDKI WRGRVNKLGFLIQ Q8A1D9 Peptidyl-prolyl cis-trans isomerase Bacteroides thetaiotaomicron VPI-5482 0.021884557 1 LEVO MSKKIYLFSLVLLALAFVSCSETEEVGKYDNWRARNEAFIDSLANVYATASGRGGLERIEMLTAPGNYIYYKEMEPMTDH VVKAGNPKYTDYVKVYYKGTNILGEYFDGNFKGDNPVVDGKDPSEGDSPTTIFQVSGVITGWGEVLQRMEVGDRWKVYIP WDYAYGSSGTTGILGYSALVFDITLLDFANTEAELK Q8A1P7 5.2.1.8 DUF4827 domain-containing protein Bacteroides thetaiotaomicron VPI-5482 0.024712709 1 LEVO MKKLVFLFLSLLAAGGIFQACDDSKTYAEMLEDEKNAVNKFIKDKGIRIISQDEFEKNDTVTNLERNEYVALSDGVYMQI VDRGSAENKTDTFANNNEICVRYIEEDIMTRDTTCFNVFLEEWGDANQLYTNPAVFRYVAEGSYVYGTFIQMDYYWASYY QSTAVPAGWLLALPFVRNYAHVRLIVPSKVGHSSAQQYVNPYYYDIWTFSKALN Q8A0S2 DUF4270 domain-containing protein Bacteroides thetaiotaomicron VPI-5482 0.058264816 1 LEVO CDDNTGGLGLGMFPGNDQNIKGKLSTFDVTTESVKTGDIYAKTNIGYIGKFTDETFGTYQAGFLAQLNCPDGLTFPEPYK EVTDASGNVISATGRMVVDDKDPENKDVTFIKDGNQIIGNIRAVELYLWYDSYFGDSLTACRLSVYELGGNGKETLNLDN AYYTDINPEDFYDSQNILGTKAYTAVDLSVKDSIRNLSTYVPSVHIAFKEDIATRVGGNILTAARKAKNADKEFNSQLFR EAFQGIYVKSDYGDGTVLYIDQPQMNVVYKCYATDSITGKKLQKKDGSGKDSTYYSYRVFATTREVIQANQLKNDPERID ALIKEDKNTYLKSPAGIFTEATLPISDIQNELTGDTLNAVKLTFTNYNQTGDKKFGMAIPSTVMLVRKKFQDSFFKDNKL SDGVSSYLTSHTSSTNQYVFSNITKLVNACIAEKEEAKKNAGSSWDETKWLQENPDWNKVVLIPVLVTYDSSNTTTGQAN IIRIQHDLKPGYVRLKGGSLGKTNPDYKLKLEVISTDFGLTTKSN Q89ZS0 N-TRIDECANOIC ACID 0.000214344 1 TDA (2S)-3-hydroxypropane-1,2-diyl dihexadecanoate 0.000568911 1 Z41 singleParticle particle 8 7.5 150.0 NaCl sodium chloride 10.0 HEPES HEPES 0.04 DDM dodecyl maltoside Quantifoil R1.2/1.3 COPPER 200 CARBON HOLEY GLOW DISCHARGE AIR ETHANE 100 277 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.8 2.0 165000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN TFS Selectris 10 FEI FALCON IV (4k x 4k) 1 13558 35.0 1 2266553 PHASE FLIPPING AND AMPLITUDE CORRECTION
Ab initio reconstruction
 2 C1 FOURIER SPACE 3.46 OTHER
The best parts of two maps at 3.28 A and 3.46 A were combined using phenix.combine_focused_maps
105155 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD
Non-uniform refinement
 emd_52209.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 448 448 448 0 0 0 448 448 448 331.52002 331.52002 331.52002 90.0 90.0 90.0 Z Y X -30.859718000000001 45.319980000000001 0.00296828 1.0508018 0.74000007 0.74000007 0.74000007 5.93 AUTHOR Composite map 9HIS PDB experimental model 9HIV PDB experimental model RIGID BODY FIT
Experimental models were fit into the combined focused map.
REAL 64.900000000000006