2025-10-22 REL PDBe PDBe PDBe 2024-12-05 2025-10-15 2025-10-15 2025-10-22 Israel Science Foundation Israel European Research Council (ERC) European Union CRYO-EM FOCUSED REFINEMENT MAP OF LEISHMANIA MAJOR 80S RIBOSOME WITH P/E-site tRNA AND mRNA : LM14Cs1H3 sKO STRAIN Rajan KS Yonath A CRYO-EM, LEISHMANIA MAJOR, 80S, RIBOSOME, LM14Cs1H3, snoRNA Bora Bhowal K Nguyen AMT Ibarra-Meneses AV Brito Lira A Rajan KS Castano JD Beaudry F Bashan A Fernandez-Prada C Olivier M Yonath A Lubell WD p -Alkoxy-Substituted Anisomycins with Potent Anti-Trypanosomiasis Activity and Expanded Modes of Action. J.Med.Chem. US 68 20264 20282 2025 40980996 doi:10.1021/acs.jmedchem.5c01291 0022-2623 0151 JMCMAR EMDB EMD-52259 associated EM volume 80S RIBOSOME WITH P/E-site tRNA AND mRNA 80S RIBOSOME WITH P/E-site tRNA AND mRNA 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 Leishmania major strain Friedlin singleParticle particle 7.6 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.5 1.5 GATAN K3 BIOQUANTUM (6k x 4k) 1.0018 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 8RXH 2.5 FSC 0.143 CUT-OFF 138498 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_52259.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 640 640 640 0 0 0 640 640 640 507.52 507.52 507.52 90.0 90.0 90.0 X Y Z -0.025154762 0.048917715 -0.00029625336 0.0017972469 0.793 0.793 0.793 0.005 AUTHOR Body 1 of focused refined map emd_52259_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 640 640 640 0 0 0 640 640 640 507.52 507.52 507.52 90.0 90.0 90.0 X Y Z -0.012279166 0.05056747 -0.0003780358 0.0017522995 0.793 0.793 0.793 AUTHOR Half map 1 for Body 2 of focused refined map emd_52259_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 640 640 640 0 0 0 640 640 640 507.52 507.52 507.52 90.0 90.0 90.0 X Y Z -0.01770505 0.0437519 -0.00045426626 0.001080179 0.793 0.793 0.793 AUTHOR Body 3 of focused refined map emd_52259_additional_3.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 640 640 640 0 0 0 640 640 640 507.52 507.52 507.52 90.0 90.0 90.0 X Y Z -0.018218707 0.04758433 -0.00039302759 0.0012877557 0.793 0.793 0.793 AUTHOR Body 2 of focused refined map emd_52259_additional_4.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 640 640 640 0 0 0 640 640 640 507.52 507.52 507.52 90.0 90.0 90.0 X Y Z -0.011935695 0.04869127 -0.00043683604 0.0014630503 0.793 0.793 0.793 AUTHOR Half map 1 for Body 3 of focused refined map emd_52259_additional_5.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 640 640 640 0 0 0 640 640 640 507.52 507.52 507.52 90.0 90.0 90.0 X Y Z -0.012154832 0.047554474 -0.0004390211 0.0014683534 0.793 0.793 0.793 AUTHOR Half map 2 for Body 3 of focused refined map emd_52259_additional_6.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 640 640 640 0 0 0 640 640 640 507.52 507.52 507.52 90.0 90.0 90.0 X Y Z -0.012825372 0.051484272 -0.00037861502 0.0017588384 0.793 0.793 0.793 AUTHOR Half map 2 for Body 2 of focused refined map emd_52259_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 640 640 640 0 0 0 640 640 640 507.52 507.52 507.52 90.0 90.0 90.0 X Y Z -0.014038771 0.052551605 -0.000291174 0.0022345919 0.793 0.793 0.793 AUTHOR Half map 1 for Body 1 of focused refined map emd_52259_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 640 640 640 0 0 0 640 640 640 507.52 507.52 507.52 90.0 90.0 90.0 X Y Z -0.01475464 0.05357134 -0.00029395407 0.0022407754 0.793 0.793 0.793 AUTHOR Half map 2 for Body 1 of focused refined map