Sub-tomogram average of the wild-type C. elegans ATP synthase dimer (narrow membrane curvature)

2025-12-17 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2024-12-05 2025-12-17 2025-12-17 2025-12-17 Biotechnology and Biological Sciences Research Council (BBSRC) BB/T008741/1 United Kingdom Sub-tomogram average of the wild-type C. elegans ATP synthase dimer (narrow membrane curvature) Buzzard E Gold VAM McLaren M Zhang D Supercomplex, Dimer, Enzyme, Respiration, MEMBRANE PROTEIN Buzzard E McLaren M Knapp-Wilson A Zhang D White R Daum B Kuwabara PE Collinson I Gold VAM The effect of NDUF-11 knockdown on respiratory chain organisation in C. elegans studied by cryo-electron tomography To Be Published 0353 EMD-52262 other EM volume

Sub-tomogram average of the wild-type C. elegans respirasome

EMD-52263 other EM volume

Sub-tomogram average of the wild-type C. elegans I1III2 respiratory supercomplex

EMD-52264 other EM volume

Sub-tomogram average of wild-type C. elegans complex I

EMD-52265 other EM volume

Sub-tomogram average of nduf-11(RNAi) C. elegans respiratory complex I

EMD-52267 other EM volume

Sub-tomogram average of the wild-type C. elegans ATP synthase dimer (intermediate membrane curvature)

EMD-52268 other EM volume

Sub-tomogram average of the wild-type C. elegans ATP synthase dimer (wide membrane curvature)

EMD-52269 other EM volume

Sub-tomogram average of the nduf-11(RNAi) C. elegans ATP synthase dimer (narrow membrane curvature)

EMD-52271 other EM volume

Sub-tomogram average of the nduf-11(RNAi) C. elegans ATP synthase dimer (intermediate membrane curvature)

EMD-52272 other EM volume

Sub-tomogram average of the nduf-11(RNAi) C. elegans ATP synthase dimer (wide membrane curvature)

EMDB EMD-52262 other EM volume

Sub-tomogram average of the wild-type C. elegans respirasome

EMDB EMD-52263 other EM volume

Sub-tomogram average of the wild-type C. elegans I1III2 respiratory supercomplex

EMDB EMD-52264 other EM volume

Sub-tomogram average of wild-type C. elegans complex I

EMDB EMD-52265 other EM volume

Sub-tomogram average of nduf-11(RNAi) C. elegans respiratory complex I

EMDB EMD-52267 other EM volume

Sub-tomogram average of the wild-type C. elegans ATP synthase dimer (intermediate membrane curvature)

EMDB EMD-52268 other EM volume

Sub-tomogram average of the wild-type C. elegans ATP synthase dimer (wide membrane curvature)

EMDB EMD-52269 other EM volume

Sub-tomogram average of the nduf-11(RNAi) C. elegans ATP synthase dimer (narrow membrane curvature)

EMDB EMD-52271 other EM volume

Sub-tomogram average of the nduf-11(RNAi) C. elegans ATP synthase dimer (intermediate membrane curvature)

EMDB EMD-52272 other EM volume

Sub-tomogram average of the nduf-11(RNAi) C. elegans ATP synthase dimer (wide membrane curvature)

EMDB EMD-52266 associated EM volume Cristae membranes isolated from wild-type C. elegans Cristae membranes isolated from wild-type C. elegans 0 Caenorhabditis elegans N2 (Bristol) Whole organism Whole organism Mitochondria subtomogramAveraging cell 10 7.0 220.0 C6H14O6 Mannitol 70.0 C12H22O11 Sucrose 5.0 C4H11NO3 Tris-HCL 1.0 C14H24N2O10 EGTA

Basic STEG/M buffer.

Quantifoil R1.2/1.3 COPPER 200 CARBON HOLEY GLOW DISCHARGE 60 AIR ETHANE 100 277.15 FEI VITROBOT MARK IV

Mitochondria isolated from C. elegans underwent freeze-thaw, to break open the outer mitochondrial membrane and thus facilitate dispersion of cristae membranes from the dense mitochondrial matrix.

TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 3.0 5.0 42000.0 NITROGEN TFS Selectris X FEI FALCON IV (4k x 4k) 1 1.41 1.31 1 C2 32.0 FSC 0.143 CUT-OFF RELION 3.1 666 100 7347 PHASE FLIPPING AND AMPLITUDE CORRECTION RELION 3.1 MAXIMUM LIKELIHOOD emd_52266.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 96 96 96 0 0 0 96 96 96 960.0 960.0 960.0 90.0 90.0 90.0 X Y Z -1.0199578 1.7537069 0.0054516844 0.08354816 10.0 10.0 10.0 0.329 AUTHOR ::::EMDATABANK.org::::EMD-52266:::: emd_52266_msk_1.map emd_52266_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 96 96 96 0 0 0 96 96 96 960.0 960.0 960.0 90.0 90.0 90.0 X Y Z -1.042457 1.7783612 0.004381475 0.12436241 10.0 10.0 10.0 AUTHOR ::::EMDATABANK.org::::EMD-52266:::: emd_52266_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 96 96 96 0 0 0 96 96 96 960.0 960.0 960.0 90.0 90.0 90.0 X Y Z -1.3328899 2.0870347 0.002657404 0.19183715 10.0 10.0 10.0 AUTHOR ::::EMDATABANK.org::::EMD-52266:::: emd_52266_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 96 96 96 0 0 0 96 96 96 960.0 960.0 960.0 90.0 90.0 90.0 X Y Z -1.3735058 1.9437429 0.006514447 0.19310933 10.0 10.0 10.0 AUTHOR ::::EMDATABANK.org::::EMD-52266::::