2026-03-04 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation.year _citation_author.identifier_ORCID _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation.year _citation_author.identifier_ORCID _em_admin.last_update PDBe PDBe 2024-12-06 2025-12-17 2025-12-17 2026-03-04 Knut and Alice Wallenberg Foundation Sweden Carlstrom A Rovsnik U Ott M Mitoribosome, translation, RIBOSOME Bridgers JB Carlstrom A Sherpa D Couvillion MT Rovsnik U Gao J Wan B Shao S Ott M Churchman LS Nat.Struct.Mol.Biol. US 33 245 258 2026 41339860 doi:10.1038/s41594-025-01726-y 1545-9985 EMD-52235 consensus EM volume

consensus map

EMD-52257 focused EM volume

LSU focused map

EMD-52252 focused EM volume

SSU_body focused map

EMD-52256 focused EM volume

SSU_head focused map

EMD-52270 focused EM volume

Aep1-Aep2-Atp25 focused map

9hlz FULLOVERLAP EMDB EMD-52235 consensus EM volume

consensus map

EMDB EMD-52257 focused EM volume

LSU focused map

EMDB EMD-52252 focused EM volume

SSU_body focused map

EMDB EMD-52256 focused EM volume

SSU_head focused map

EMDB EMD-52270 focused EM volume

Aep1-Aep2-Atp25 focused map

EMDB EMD-52281 associated EM volume

Translational activators Aep1, Aep2 and Atp25 in complex with mRNA and the yeast mitochondrial ribosome

Aep1, Aep2 and Atp25 in complex with the 74S mitochondrial ribosome Aep1, Aep2 and Atp25 in complex with the 74S mitochondrial ribosome 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 Saccharomyces cerevisiae W303 54S mitoribosomal large subunit 1 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 70 71 72 73 74 75 76 77 78 Saccharomyces cerevisiae W303 Aep1, Aep2 and Atp25 bound to the 37S mitoribosomal small subunit 1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 69 79 Saccharomyces cerevisiae W303 Small ribosomal subunit protein mS38 Saccharomyces cerevisiae W303 0.012800425999999998 1 LEVO MLGRALRPGWLGITRTVVKKPSCGSYFNRTFQTAINTTMPPMQEGMLSTMMMMTATATRITGTVSEPLNGSNIVMQLDSV MRKRKKKMKKHKLRKRRKREKAERRKLSQGR P32344 Small ribosomal subunit protein mS41 Saccharomyces cerevisiae W303 0.015321683999999999 1 LEVO MIPSRISHKFPLFLRSSLAAPKAAYRFSSTIPKPSDQVPDVDAFLNKIGRNCNELKDTFENNWNNLFQWDSKILKEKGVN IQQRKYILKQVHNYRNNRPIHEIKLGKKSFFGGERKRKAFTAKWKAENKQ P38783 Small ribosomal subunit protein mS42 Saccharomyces cerevisiae W303 0.030257449 1 LEVO MLVFKRGIHVVPKLPNSKALLQNGVPNILSSSGFKTVWFDYQRYLCDKLTLATAGQSLESYYPFHILLKTAGNPLQSNIF NLASSIHNNHLFVENILPSAVEHGTNSNAVVKTEPSRLFLSKIKDSFNGSDWEVVKEEMIYRAENEVLGQGWLFLVENNE KKLFILTSNNNGTPYYFPRNQSFDLNSAISIDEFATLKQMKELIGKSTKLNGKVQDWTMPIICVNLWDHAYLHDYGVGNR SKYVKNVLDNLNWSVVNNRIFSGISK P47141 Small ribosomal subunit protein mS43 Saccharomyces cerevisiae W303 0.036775781 1 LEVO MLRFTGARAIRKYSTRYALEHLKEGAPLKGLFSIEGLQKAWFDRVKYLDAKLNDCTNEAQQKPLETLIHENSKSASKKHI VNYASSLYNLKFSMSSLQGCIRTPPEECPRLGPEALLQTPDFNRTISNEPLTTGNERLQAALISSFGSLMEFRTLLINSN LAISGDGFTWLVARRQLDKRAMRNDMPNRDIEYDKLFILNTYNAGTPFNFSTSGVMNELNNQYTNMEKQRAKEAGNLEDS EMTAKQAKTKFIYETQQKGFSGKEVSYIPLLAIDASPKTWLTDYGVFGKREYLERVWDSIEWKIVESRLPQRTKIQAFNT L Small ribosomal subunit protein mS44 Saccharomyces cerevisiae W303 0.039045031 1 LEVO MGTITVVINEGPILLIRALHRATTNKKMFRSTVWRRFASTGEIAKAKLDEFLIYHKTDAKLKPFIYRPKNAQILLTKDIR DPKTREPLQPRPPVKPLSKQTLNDFIYSVEPNSTELLDWFKEWTGTSIRKRAIWTYISPIHVQKMLTASFFKIGKYAHMV GLLYGIEHKFLKAQNPSVFDIEHFFNTNIMCALHRNRLKDYKDAEIAQRKLQVAWKKVLNRKNNTGLANILVATLGRQIG FTPELTGLQPVDISLPDIPNSSSGAELKDLLSKYEGIYLIARTLLDIDQHNAQYLELQEFIRQYQNALSESSDPYDTHLK ALGLLETPPPQESTEKEEK Small ribosomal subunit protein mS45 Saccharomyces cerevisiae W303 0.039638172

Small ribosomal subunit protein mS45

1 LEVO MSYGLTGTSSKLRGTSSIFSWTQVRHFSRRRIAYPFYPFKKLGRQHPKKHDTNLKTAMRQFLGPKNYKGEYVMNKYFTVP TNHVPNYIKPDLERGQSLEHPVTKKPLQLRYDGTLGPPPVENKRLQNIFKDRLLQPFPSNPHCKTNYVLSPQLKQSIFEE ITVEGLSAQQVSQKYGLKIPRVEAIVKLVSVENSWNRRNRVSSDLKTMDETLYRMFPVFDSDASFKRENLSEIPVPQKTL ASRFLTIAESEPFGPVDAAHVLELEPAVETLRNLSTVGEHSSGHQQSTNKNTKVIYGELVEGERSQYKFTNAKVGKVGYR YGSGNRDNKKDRRIGFNKLGQMVYI Small ribosomal subunit protein mS46 Saccharomyces cerevisiae W303 0.041281968999999995 1 LEVO MRSSMFRCVSRAHYSTSVTEDFINSILARAQEATAKASSNALKLDKMKEGRMQNKRRNGNQNRNSMNNKESRGREGNQGE RNMRLKNRSSDSVRANKQQWNKGANTSFVKNPTGNTVVMQPQFKKMQNGKNNLKGDARVEDDLLDVFNSSMEQKPVNFNG TPKSKARFQKKSHILTASKRRKAPQQQLQKVIKRPVSSEYVLEEPTPLSLLEYTPQVFPTKESRLVNFTLDSLKKSNYPI YRSPNLGILKVHDFTLNTPNFGKYTPGSSLIFAKEPQLQNLLIEEDPEDFHRQVTGEYQLLKPYVKKDFEKLTKSKDTVS KLVQNSQVVRLSLQSVVMGSEEKKLVYDVCSGMKPISELQQ Small ribosomal subunit protein mS47 Saccharomyces cerevisiae W303 0.056351195 1 LEVO MLRNTLKCAQLSSKYGFKTTTRTFMTTQPQLNVTDAPPVLFTVQDTARVITLNRPKKLNALNAEMSESMFKTLNEYAKSD TTNLVILKSSNRPRSFCAGGDVATVAIFNFNKEFAKSIKFFTDEYSLNFQIATYLKPIVTFMDGITMGGGVGLSIHTPFR IATENTKWAMPEMDIGFFPDVGSTFALPRIVTLANSNSQMALYLCLTGEVVTGADAYMLGLASHYVSSENLDALQKRLGE ISPPFNNDPQSAYFFGMVNESIDEFVSPLPKDYVFKYSNEKLNVIEACFNLSKNGTIEDIMNNLRQYEGSAEGKAFAQEI KTKLLTKSPSSLQIALRLVQENSRDHIESAIKRDLYTAANMCMNQDSLVEFSEATKHKLIDKQRVPYPWTKKEQLFVSQL TSITSPKPSLPMSLLRNTSNVTWTQYPYHSKYQLPTEQEIAAYIEKRTNDDTGAKVTEREVLNHFANVIPSRRGKLGIQS LCKIVCERKCEEVNDGLRWK Small ribosomal subunit protein bS1m Saccharomyces cerevisiae W303 0.039507457 1 LEVO MTLAELLGRSRIAQVANNHKPLTYTGKKFHPTHQIIETKPSTLYRQEWGLKSAIPSKIKSRYLVYNDLDTLERITTFEPR GGTQWNRLRFQEMGVPIVSNIGRQNPFFKYISRPEDESHAKLSLFKEMKGDTDISPAAMKKRLKKITALIRSFQDEFKEW LVENHPDELKLNSNKLEDYVVKFLNKKLETKTNKKFNTEIIGTGGLSYSLPGKLKNSPNGVIQRTVVPGRILNVVKENND NKWLAAIGGFVADVVFFQSPPSSFNSMGDFIRMKTFLFEILEASMEKNGSVSMHARLLEPQNDKTREFFNKRPIYKPLTS RRARRPSVGNIQEANNLLNIIKGN Small ribosomal subunit protein uS2m Saccharomyces cerevisiae W303 0.044205727 1 LEVO MQRHVFARNFRRLSLLRNPSLTKRFQSSASGAANTPNNNDEVMLLQQKLLYDEIRSELKSLSQVPEDEILPELKKSLEQD KLSDKEQQLEAELSDFFRNYALLNKLFDSKTLDGQSSTTTAAATPTKPYPNLIPSANDKPYSSQELFLRQLNHSMRTAKL GATISKVYYPHKDIFYPPLPENITVESLMSAGVHLGQSTSLWRSSTQSYIYGEYKGIHIIDLNQTLSYLKRAAKVVEGVS ESGGIILFLGTRQGQKRGLEEAAKKTHGYYVSTRWIPGTLTNSTEISGIWEKQEIDSNDNPTERALSPNETSKQVKPDLL VVLNPTENRNALLEAIKSRVPTIAIIDTDSEPSLVTYPIPGNDDSLRSVNFLLGVLARAGQRGLQNRLARNNEK Small ribosomal subunit protein uS3m Saccharomyces cerevisiae W303 0.047170996 1 LEVO MKLKLLNMILSMMNKTNNNNNIIINNTLDSLMNKKLLLKNMLLDMNNKKMNNMKRMLNNNNMNPAGANPVVHRIGPAGNI NNKLQHLNNMNNWNTQIYNYNKNMEIMNTMNDKLINKLLYKMMTLKLNNMNINKIIMSKTINQHSLNKLNIKFYYYNNDI NNNNNNNNNNYYMNMMNKLMNIMNNNMNNNLCNILSYYYKKKVTIEPIKLSYIYLNSDIFSKYISLNDMDKYNNGILTNY QRMLNNIMPKLNDHNISMNYINNINNINNNKYNNMINLLNNNNNINNNNNYNNNNNNYIGNINNIYNNMTIDNIPMDILM YKYLVGWSIKFKGRLSNNNGRTSTTNLLNGTFNNKKYLWSNINNNYKLNYIPSNHNLYNNSNINKNGKYNIKVKLNFI Small ribosomal subunit protein uS4m Saccharomyces cerevisiae W303 0.056450949 1 LEVO MPRKANLLKSLARGRVRTSFNKYNLFNLYKKGGVDLKSKSLYQQKWTAKQETRAYHGEHLTEKRWQTVFKPKLDSVAQLD ASLRGGEIKETPFLLQTFAVLEKRLDFALFRAMFASSVRQARQFILHGNVRVNGVKIKHPSYTLKPGDMFSVKPDKVLEA LGAKKPSFQEALKIDKTQIVLWNKYVKEAKTEPKEVWEKKLENFEKMSDSNPKKLQFQEFLRQYNKNLESQQYNALKGCT QEGILRKLLNVEKEIGKSNNEPLSIDELKQGLPEIQDSQLLESLNNAYQEFFKSGEIRREIISKCQPDELISLATEMMNP NETTKKELSDGAKSALRSGKRIIAESVKLWTKNITDHFKTRMSDISDGSLTFDPKWAKNLKYHDPIKLSELEGDEPKARK LINLPWQKNYVYGRQDPKKPFFTPWKPRPFLSPFAILPHHLEISFKTCHAVYLRDPVARPGQSEVISPFDVPVHERAYMY YLRNGK Small ribosomal subunit protein uS5m Saccharomyces cerevisiae W303 0.034941101999999995 1 LEVO MFKRQLSTSVRYLQHYDESLLSRYYPESLLKSIKLAQQTIPEDTKFRVSRNVEFAPPYLDDFTKIHPFWDYKPGMPHLHA QEENNNFSIFRWDQVQQPLPGEGNILPPGVSLPNDGGRKSKSADVAAGLHKQTGVDPDYITRKLTMKPLVMKRVSNQTGK GKIASFYALVVVGDKNGMVGLGEGKSREEMSKAIFKAHWDAVRNLKEIPRYENRTIYGDIDFRYHGVKLHLRSAKPGFGL RVNHVIFEICECAGIKDLSGKVYKSRNDMNIAKGTIEAFTKAQKTLDEVALGRGKKLVDVRKVYYSS Small ribosomal subunit protein bS6m Saccharomyces cerevisiae W303 0.015046701999999999 1 LEVO MLYELIGLVRITNSNAPKLEAKELSSTIGKLIIQNRGVVRDIVPMGIRYLPKIMKKDQEKHFRAYHFLMLFDSSAAVQSE ILRTLKKDPRVIRSSIVKVDLDKQLDRASSLHRSLGKKSILELVNEDYQSI Small ribosomal subunit protein uS7m Saccharomyces cerevisiae W303 0.027857141 1 LEVO MLHCARRYMLVRPRLLWQSGTCVARFQSSVRTPASEPSAEKGVDEWLEAINELREEFSAKEYLPETSLAPPGQSKVDLLQ GSQAGSKIKPTAEQLAQWEALKSVPIPPRKNATLDHITNMIMRHGKKEKAQTILSRALYLVYCQTRQDPIQALEKSLDEL APLMMTKTFNTGVAKASVIPVPLNKRQRNRIAWNWIVQSANQRVSSDFAVRLGEELTAIAKGTSSAFEKRDQIHKTAIAH RAYIQLK Small ribosomal subunit protein uS8m Saccharomyces cerevisiae W303 0.017496601999999997 1 LEVO MSLVKLANTCAHLQNCSKVRVALTSIPYTKLQLQFAYNLYQQGFLSSLQKGSTMGPDKDFVEVTPDNISTRRLWVGLKYR DNKPVLSSCKLISKPNSRIHLPMEDMKKLCSGVTIRNIKPLQPGELILVRAHNNIMDINEAISKKLDGEVLCRVK Small ribosomal subunit protein uS9m Saccharomyces cerevisiae W303 0.031980258 1 LEVO MFSRLSLFRRAALAPAPMRMSFRTIYQKTEDELPRRIVPKLATFYSANPNHEDRINRLERLLRKYIKLPSQNNNEAQQTK APWISFDEYALIGGGTKLKPTQYTQLLYMLNKLHNIDPQLTNDEITSELSQYYKKSSMLSNNIKIKTLDEFGRSIAVGKR KSSTAKVFVVRGTGEILVNGRQLNDYFLKMKDRESIMYPLQVIESVGKYNIFATTSGGGPTGQAESIMHAIAKALVVFNP LLKSRLHKAGVLTRDYRHVERKKPGKKKARKMPTWVKR Small ribosomal subunit protein uS10m Saccharomyces cerevisiae W303 0.023458919999999998 1 LEVO MLRNTIALRSFIRTQSTRPYPVNVEAVYYAPLKLPIKYGDLVADIQLRSYDNENLDFYSDFILRTGYYLGIPLTGPKPLP TRRERWTVIKSPFVHAKSKENFERHTHKRLIRAWDTNPEVLQMLIAYITKHSMAGVGMKCNFFQRSEISLDLGSDANGLE KSLSNIDELYSLRNDDKAQTSAVGQKVLELLDSPDFKKHLEKK Small ribosomal subunit protein uS11m Saccharomyces cerevisiae W303 0.024603419999999997 1 LEVO MLLQPVWKGCRWTQFVRPIRRWNSTGTNRGVPFSFKDISNQEDITNISYPSSSDSVLTKSNGSSEVYKPKEEVVKYILHG KFTKNNTHLTFSSVVEDKNFHKNKGLTYNDTMLYYLNLPQKVKISLSTGCLGFRKAARGEYEAAFQTSGRMFELIKEKNM LNKDIEVVMDDFGKGRAAFISALVGKEGASVVKKVVKISDATKLKFGGVRSPKMRRL Small ribosomal subunit protein uS12m Saccharomyces cerevisiae W303 0.01694874 1 LEVO MLSRFMSNTWCTPLRQAQRLFSSTTTMQATLNQIKRGSGPPRRKKISTAPQLDQCPQRKGVVLRVMVLKPKKPNSAQRKA CRVRLTNGNVVSAYIPGEGHDAQEHSIVYVRGGRCQDLPGVKYHVIRGAGDLSGVVNRISSRSKYGAKKPSKS Small ribosomal subunit protein uS13m Saccharomyces cerevisiae W303 0.016087031 1 LEVO MVVHILGKGFKGKEVIKIALASKFYGIGKTTAEKICSKLGLYPWMRMHQLSEPQIMSIASELSTMTIEGDARAIVKDNIA LKRKIGSYSGMRHTLHLPVRGQHTRNNAKTARKLNKIDRRGIHTFSQAKVQHNPSLWSCIFGK Small ribosomal subunit protein uS14m Saccharomyces cerevisiae W303 0.013563061 1 LEVO MGNFRFPIKTKLPPGFINARILRDNFKRQQFKENEILVKSLKFIARNMNLPTKLRLEAQLKLNALPNYMRSTQIKNRCVD SGHARFVLSDFRLCRYQFRENALKGNLPGVKKGIW Small ribosomal subunit protein uS15m Saccharomyces cerevisiae W303 0.033116383 1 LEVO MSIVGRNAILNLRISLCPLFMGKRSFVSSPVSNSAKAVKFLKAQRRKQKNEAKQATLKASTDKVDPVLGRADTPFITRIM AELKEPLVLSKGYNIEEVDKFLAAIESAKRERAELSGLNTEVVGIEDIEKLEDRREAILRILSMRNSENKNAIKMAVELA RKEFERFPGDTGSSEVQAACMTVRIQNMANHIKEHRKDFANTRNLRILVQQRQAILRYLKRDNPEKYYWTIQKLGLNDAA ITDEFNMDRRYMQDYEFFGDKILIRDSKKVANQKRKEIRKQKRATF Small ribosomal subunit protein bS16m Saccharomyces cerevisiae W303 0.013663011 1 LEVO MTCGLVRIRLARFGRKNSPVYNIVVANSRKARDAKPIEVLGTYVPVPSPVTKRELKRGVVPIKDVKLDFDRTKYWIGVGA QPSETVTKLLRKAGILNDAWATSKNSNVNRKVVFERMETLE Small ribosomal subunit protein uS17m Saccharomyces cerevisiae W303 0.027683986 1 LEVO MARQNFLGLVVSQGKMQKTVKVRVETKVFNKKINKELFHRRDYLVHDEGEISREGDLVRIEATRPLSKRKFFAIAEIIRN KGQQFALYESEAQLSVAKEEAQKAKEFLDKRSVRENKLNEKTTLLRDIRTIQDALSSGSTPKELLEIKQRYGIQDFSQET VRQLLQLDISGLEVNLEKQRSLIDRIQTRLSELLSNDLKCDQFLKDHGVEDPLTLKKNIKKNLLRKHVMMDMQQPSQ Small ribosomal subunit protein bS18m Saccharomyces cerevisiae W303 0.01586649 1 LEVO MQPIIKGAVSSTFKRALYNFGIKEKKSVNIEMGRTQQTKKIDQSLSKKLPKGTIYDPFDFSMGRIHLDRKYQANKNSNRN DIMKSGANPLEFYARPRILSRYVTSTGRIQHRDITGLSAKNQRRLSKAIRRCQAIGLM Small ribosomal subunit protein uS19m Saccharomyces cerevisiae W303 0.010292044 1 LEVO MQPAARLLSRSVWKGPNIVPLPIREAMTKGTPIRTNARAATILPQFVGLKFQIHNGKEYVPIEISEDMVGHKLGEFAPTR KRFSYTQTKNK Small ribosomal subunit protein bS21m Saccharomyces cerevisiae W303 0.020432744 1 LEVO MLKSTLRLSRISLRRGFTTIDCLRQQNSDIDKIILNPIKLAQGSNSDRGQTSKSKTDNADILSMEIPVDMMQSAGRINKR ELLSEAEIARSSVENAQMRFNSGKSIIVNKNNPAESFKRLNRIMFENNIPGDKRSQRFYMKPGKVAELKRSQRHRKEFMM GFKRLIEIVKDAKRKGY Small ribosomal subunit protein mS23 Saccharomyces cerevisiae W303 0.030554354 1 LEVO MKIQTNAVNVLQRTSAYLKSGLLKETPAWYNVVASIPPSTKFTREPRFKNPSNGHIIGKLVDVTEQPHANNKGLYKTRPN SSDKRVGVKRLYRPPKLTYVEDRLRSLFYKQHPWELSRPKILVENEIGDENYDWSHMLQIGRPLDGESVIQRTMYLIKTK QYGDMVEAYDHARYEFYALRMQEETEQQVALEEAEMFGSLFGVSAIEHGIQKEQEVLDVWEKKVVEETELMAARTSNPAG SWKDDTTLNTAQEEESTTSENLHF Small ribosomal subunit protein mS29 Saccharomyces cerevisiae W303 0.050940742 1 LEVO MLRMSTSRFIGQRLFTTARSLQAAKPAPKGKTQGFSKKSSSVSSYSSAKRVTPGSLYKNWTNTTHTAQLQQTAVPLALPI FNFDDISKTLNKVVSYSNKQYKSLHHLGSFKKSQFNELFQKPVCLVREDATNSFLKKLVSHPVKKFIITGEPGVGKTVLL SQAHAYAVDSKQIIINISYPELFLNGRNDFSYDDDLKLFIQPMYLKKLIRKILKANDPALLKSIELSKDYKFSNANPKNA SVKPFVTLNKTKNTVLDLLSVMTHPHNRGKLMKAIIDELSVQSKVPIMFTVDNFSKVLTTAYSAYRNTENKQIYSLDLQM GKLMMDIISGETKFANGESSTILAISGVDRTNKTLPVALGKIPVDPYVTRYHYEPKFVELLQKGNVTEFEVPKLNKQEVN ELIDYYKQSNVLLDKDITGKKWENLIDEKYFLSGNGNPRELLKSLVLSHR Small ribosomal subunit protein mS33 Saccharomyces cerevisiae W303 0.012420665999999999 1 LEVO MNVPKARLLKVAELSAKIFDQNFNPSGIRTGSKILNERLKGPSVASYYGNPDILKFRHLKTLYPDIEFVDLEEQYRLSMV EAKKRRGKGAPKKMKKDAAATAKGKGKKKK Small ribosomal subunit protein mS35 Saccharomyces cerevisiae W303 0.037458496 1 LEVO MKVPLGLWKVSRGNLWSTQKRVLTMSRCLNSDAGNEAKTVREGPAFSADLYMHPEKWKGLPPQRILELYWERMARLGSEY KPNKDELNALLTTSEYSNVPVNDIKKLYHRGEQGAIDIKGGNVNRDNSLRPFMFDELPSQAQELVAQHREQRFYNRLAAY ELPLLAQYRQEYKRPSPESHPVTYRYTSYVGEEHPNSRKVVLSVKTKELGLEEKSLHKFRILARSRYDHTTDIFKMSSDK FEHASQNARYLHDILQRLLAESKDLTEDDFSDVPLDTRHTIAKSLRKKKRDYEFPEHWKRPEDAPKKKFDIVDQLLSTL Small ribosomal subunit protein mS37 Saccharomyces cerevisiae W303 0.010709597999999999 1 LEVO MSGKPPVYRLPPLPRLKVKKPIIRQEANKCLVLMSNLLQCWSSYGHMSPKCAGLVTELKSCTSESALGKRNNVQKSNINY HAARLYDRINGKPHD ATPase expression protein 1 Saccharomyces cerevisiae W303 0.059830516 1 LEVO MITTVQEISKWRNLCFIRMQSRKWYPVLKKTPLVADGRKIIKHADKVPHPEEIIHPFYQPTAIEQFTACATEYNPSLLDG KKIAPSLIKHPVSLKTILVDSKLKFDDIRGVNKWLMEFVARRQHQRNIVLTPASKSVRSFHVLHLSSTDIAKLRGLENIL SEIENTNDLQSRVESVNNELQNIFDRDSKQTRLFCENILAYLIKNYGNSTEKLILLINVTEMQLYSRLDQMKAMNIILYN ILCKVEANENPPYSPTLVTALENLLAAINNRFFPGRCENSLHPIVIEQLLSYFIKTGNLNESKNFLGHLIKKGILPEATI INRYLEAIDVHFDKSTKIFDIRSKFAFIADLAPIIENYGTIDLFKFLIPMCRHFDELCSLLNIIRKSNNAKRAVDSTLPI FIKKVLTFTKDPMINSGNLSTVFNIVSPIYGQNVPSEFVEKFILSFALQGNYTMMAHMIDTYKIKLSHKYQLQIIRALKN SERNHALKNTGAVGYNKEFKKYFIEKYLNCTEREALRP ATPase expression protein 2 Saccharomyces cerevisiae W303 0.06763246099999999 1 LEVO MWINRLVKHPSYSVLRFYTKRLCTVSVKSLREFGVLPNSTICHSVYPRRTYVMGRAVINDILIKKSYSTHTVCAIDRSKD ENNGSAYDKFEAKGIPIDVHTLKRIISSSGMDESEFSKSISYLFAKTVDPEPKDVLSLEDLSFLLSKLYTQRFQIRRICR DINAKYSEFWFKLFSLYAEKVDAKRNQVNLRNTKLDACEIFDANLMIKNFIELGQLGKAQKILSFILDRNPDILLSPKNA DISTIVHFLQLRCGALAPYWKIPDNSEQKQGFLRKMVRLGAKNTSIRLSSTYKAMDHQTLLKIADLALQEKKLLNSEDLL STLIQSFGHLGQTQILERCIEHIWQISPQEFPSHVVIKHRGCYPSSKILVSILVSFYFNDHDLHRGLSILDSFIKHYPDV KLDALFWRRLFQLSHFAWTPANDKKATSVVRCWHLMKQWYASKRLRPSVDYETLRQLYDIMKKTGNFPLGIDVLRSFKPG IERTRAENAGKVNNIIIKYQKCIIKELVNRGRFSAVREFIDSYGFDRKMTKDLNIFCANRMFLRSKKMKNKIENKKEREK VRLDSFDDDEDDGMIIGSLW ATPase synthesis protein 25, mitochondrial Saccharomyces cerevisiae W303 0.070517625 1 LEVO MNKFCLLPFHGKRIGVANIPFTILFKKGPYFLHSHITAVYYSTKGKNDSHEQSRVSKKSTFTPLETPWYLRIVDNEKELM EGKKNNHHTMNKELEIPKTSPNSLRKIADLLTGKLGLDDFLVFDLRKKSPNSVSAVNKLGDFMVICTARSTKHCHKSFLE LNKFLKHEFCSSAYVEGNFNERQESRRKRRLARKSNLSKLLGRSSECSAKDLNSEAWYMIDCRVDGIFVNILTQRRRNEL NLEELYAPENEKSKFQNIDSGNVPTISGVNEISSNNNILLGLRRLAQQRRRYSTINPNGLSNLRYFLQKEDFKGANKIIQ SSSGTETHNIRTLEHVKNTLKDLVGQERKVDVVQWKSLFDEHSTFLTINQSAAYWPLRLEYAILLNKADPQFYSDRVFLK DYLLLKKSLGQELIREDLIALLEMVLKTQHSSHSYFNLVKQNRVIIRALNLFKGLQTEDDGSVVYDEVVISLLLNSMVAD ERVKLRSLYETIDHIFQTFGDKLTSGMIVSILQNLAKIKDWNKLLQVWEAITPTEGEGQDKRPWNEFINVINQSGDSHVI SKIVNNGHLLWIRRLNVNVTPELCNSIKALLKTAGMENSTLEEFLVRGTNNQ E/E-site formyl-methionine tRNA Saccharomyces cerevisiae W303 0.024234259999999997 1 UGCAAUAUGAUGUAAUUGGUUAACAUUUUAGGGUCAUGACCUAAUUAUAUACGUUCAAAUCGUAUUAUUGCUACCA Large ribosomal subunit protein bL36m Saccharomyces cerevisiae W303 0.010718977999999999 1 LEVO MFLQTLRLTMPRMFLHMKPSPITITRACTVPSLLSVAAPQPALVAANRPLVFNRGFKVRTSVKKFCSDCYLVRRKGRVYI YCKSNKKHKQRQG O14464 Large ribosomal subunit protein mL38 Saccharomyces cerevisiae W303 0.042886059 1 LEVO MLRRSIHTTKILQKPNATSHIWSDFTTRPSSLSIQSSKVKNYLFQKKASLDPPSISRRSNRIKYSPPEHIDEIFRMSYDF LEQRSSKFYELANKTKNPLKKDALLIKAEINNPEVQYNFQFNNKLNNVKDIIDYDVPVYRHLGKQHWESYGQMLLMQRLE TLAAIPDTLPTLVPRAEVNIKFPFSTGVNKWIEPGEFLSSNVTSMRPIFKIQEYELVNVEKQLYTVLIVNPDVPDLSNDS FKTALCYGLVNINLTYNDNLIDPRKFHSSNIIADYLPPVPEKNAGKQRFVVWVFRQPLIEDKQGPNMLEIDRKELSRDDF DIRQFTKKYNLTAIGAHIWRSEWDAKVAAVREKYGLPPGRVFSRVRR Q06678 Large ribosomal subunit protein mL40 Saccharomyces cerevisiae W303 0.017373252 1 LEVO MLAQTFKKPHRAVLEQVSGTTVFIRNKRTKSKSSLSPLAQRVVTQLSVMSASRKQPKLLKLAREDLIKHQTIEKCWSIYQ QQQRERRNLQLELQYKSIERSMNLLQELSPRLFEAANASEKGKRFPMEMKVPTDFPPNTLWHYNFRK P36527 Large ribosomal subunit protein mL41 Saccharomyces cerevisiae W303 0.016521102 1 LEVO MKGSPISQFSKTSINALTRPWKKYRDGELFYGLSKVGNKRVPLTTKQGNKTMYKGTRASGIGRHTKFGGYVINWKKVRTY VTPDMVNFELKPYVNANVPPLKHEFKGFSGGPLDPRLQLLKIKEYIVNGRVQSEGATDTSCYKERG Large ribosomal subunit protein mL43 Saccharomyces cerevisiae W303 0.016153749 1 LEVO MVVKAIARNSIGRNGVGAFVFPCRKITLQFCNWGGSSEGMRKFLTSKRLDKWGQEFPWIQFEVMRKSGHPLLRAEYTNGR EKVICVRNLNIDNVENKLKLLKDSDGDILRRRTKNDNVESLNSSVRGIWSPLHAAKRHRI Large ribosomal subunit protein mL44 Saccharomyces cerevisiae W303 0.044058742 1 LEVO MGIVLKRAIAAGMKPFPNSTWHWSRTIRPFSQHLSSTCFLQQSSRFTSKRYLHLSTLTQQEKRFLPESELAKYKEYYQGL KSTVNEIPESVASKSPSLRTLHKRLQLPNELTYSTLSRCLTCPSAKLPDKINNPTKGAAFVNTVPTNKYLDNHGLNIMGK NLLSYHVTKSIIQKYPRLPTVVLNAAVNAYISEAVLAHIAKYWGIEVETTSVLSRYLKMEPFEFTLGRLKFFNNSLNSKD GIELITGKNFSETSALAMSVRSIIAAIWAVTEQKDSQAVYRFIDDHIMSRKLDITKMFQFEQPTRELAMLCRREGLEKPV SKLVAESGRLSKSPVFIVHVFSGEETLGEGYGSSLKEAKARAATDALMKWYCYEPLAQQEPVIDPGTVVV Large ribosomal subunit protein mL46 Saccharomyces cerevisiae W303 0.03226174 1 LEVO MKVNLMLKRGLATATATASSAPPKIKVGVLLSRIPIIKSELNELEKKYYEYQSELEKRLMWTFPAYFYFKKGTVAEHKFL SLQKGPISKKNGIWFPRGIPDIKHGRERSTKQEVKLSDDSTVAFSNNQKEQSKDDVNRPVIPNDRITEADRSNDMKSLER QLSRTLYLLVKDKSGTWKFPNFDLSDESKPLHVHAENELKLLSGDQIYTWSVSATPIGVLQDERNRTAEFIVKSHILAGK FDLVASKNDAFEDFAWLTKGEISEYVPKDYFNKTEFLLADN Large ribosomal subunit protein mL49 Saccharomyces cerevisiae W303 0.016406219 1 LEVO MISSCVTRCFGRGKCLPGPATASIYQTIRCISTNSNKAAEAPIFPKLEDVKMHELIGNNNFGKKTYYVERSRTGNLPVYS AYKNGGNKIITEIRKIEGDVIQLRNDLQEQLPFIPKKSWSVVMQSKKIIIKGNAVEAVKRVLTKKF Large ribosomal subunit protein mL50 Saccharomyces cerevisiae W303 0.030317365 1 LEVO MSSLLKLHCIRPLPQRSVWLSGYKQKARCIHSSAANGDFMSWFKRKKQEEHQEPVKDTKQLIKDIEEGTNEASSQSSSNN KNRLELIPENFIGEGSRRCKRQKELKLAVSSAPFNQWLSRDKITSDNQLDDMILQATEKTLGKVDQDVQFSDLVAKFQFT KFLQSKSGYLIPDYELTTLSTPLQFKRYIKEKILPSANDPKLAYKEAEPNAIHPFSDNYASPNIYVVNDVTSKEQKSKYD TIMKEIQKLEDDATRKALETARSA Large ribosomal subunit protein mL57 Saccharomyces cerevisiae W303 0.028206495999999998 1 LEVO MENSMMFISRSLRRPVTALNCNLQSVRTVIYLHKGPRINGLRRDPESYLRNPSGVLFTEVNAKECQDKVRSILQLPKYGI NLSNELILQCLTHKSFAHGSKPYNEKLNLLGAQFLKLQTCIHSLKNGSPAESCENGQLSLQFSNLGTKFAKELTSKNTAC TFVKLHNLDPFIFWKMRDPIKDGHINGETTIFASVLNAFIGAILSTNGSEKAAKFIQGSLLDKEDLHSLVNIANENVASA KAKISDKENKAFL 21S mitochondrial rRNA Saccharomyces cerevisiae W303 1.057805375 1 GUAAAAAGUAGAAUAAUAGAUUUGAAAUAUUUAUUAUAUAGAUUUAAAGAGAUAAUCAUGGAGUAUAAUAAUUAAAUUUA AUAAAUUUAAUAUAACUAUUAAUAGAAUUAGGUUACUAAUAAAUUAAUAACAAUUAAUUUUAAAACCUAAAGGUAAACCU UUAUAUUAAUAAUGUUAUUUUUUAUUAUUUUUAUAAUAAGAAUAAUUAUUAAUAAUAAUAAACUAAGUGAACUGAAACAU CUAAGUAACUUAAGGAUAAGAAAUCAACAGAGAUAUUAUGAGUAUUGGUGAGAGAAAAUAAUAAAGGUCUAAUAAGUAUU AUGUGAAAAAAAUGUAAGAAAAUAGGAUAACAAAUUCUAAGACUAAAUACUAUUAAUAAGUAUAGUAAGUACCGUAAGGG AAAGUAUGAAAAUGAUUAUUUUAUAAGCAAUCAUGAAUAUAUUAUAUUAUAUUAAUGAUGUACCUUUUGUAUAAUGGGUC AGCAAGUAAUUAAUAUUAGUAAAACAAUAAGUUAUAAAUAAAUAGAAUAAUAUAUAUAUAUAAAAAAAUAUAUUAAAAUA UUUAAUUAAUAUUAAUUGACCCGAAAGCAAACGAUCUAACUAUGAUAAGAUGGAUAAACGAUCGAACAGGUUGAUGUUGC AAUAUCAUCUGAUUAAUUGUGGUUAGUAGUGAAAGACAAAUCUGGUUUGCAGAUAGCUGGUUUUCUAUGAAAUAUAUGUA AGUAUAGCCUUUAUAAAUAAUAAUUAUUAUAUAAUAUUAUAUUAAUAUUAUAUAAAGAAUGGUACAGCAAUUAAUAUAUA UUAGGGAACUAUUAAAGUUUUAUUAAUAAUAUUAAAUCUCGAAAUAUUUAAUUAUAUAUAAUAAAGAGUCAGAUUAUGUG CGAUAAGGUAAAUAAUCUAAAGGGAAACAGCCCAGAUUAAGAUAUAAAGUUCCUAAUAAAUAAUAAGUGAAAUAAAUAUU AAAAUAUUAUAAUAUAAUCAGUUAAUGGGUUUGACAAUAACCAUUUUUUAAUGAACAUGUAACAAUGCACUGAUUUAUAA UAAAUAAAAAAAAAUAAUAUUUAAAAUCAAAUAUAUAUAUAUUUGUUAAUAGAUAAUAUACGGAUCUUAAUAAUAAGAAU UAUUUAAUUCCUAAUAUGGAAUAUUAUAUUUUUAUAAUAAAAAUAUAAAUACUGAAUAUCUAAAUAUUAUUAUUACUUUU UUUUUAAUAAUAAUAAUAUGGUAAUAGAACAUUUAAUGAUAAUAUAUAUUAGUUAUUAAUUAAUAUAUGUAUUAAUUAAA UAGAGAAUGCUGACAUGAGUAACGAAAAAAAGGUAUAAACCUUUUCACCUAAAACAUAAGGUUUAACUAUAAAAGUACGG CCCCUAAUUAAAUUAAUAAGAAUAUAAAUAUAUUUAAGAUGGGAUAAUCUAUAUUAAUAAAAAUUUAUCUUAAAAUAUAU AUAUUAUUAAUAAUUAUAUUAAUUAAUUAAUAAUAUAUAUAAUUAUAUUAUAUAUUAUAUAUUUUUUAUAUAAUAUAAAC UAAUAAAGAUCAGGAAAUAAUUAAUGUAUACCGUAAUGUAGACCGACUCAGGUAUGUAAGUAGAGAAUAUGAAGGUGAAU UAGAUAAUUAAAGGGAAGGAACUCGGCAAAGAUAGCUCAUAAGUUAGUCAAUAAAGAGUAAUAAGAACAAAGUUGUACAA CUGUUUACUAAAAACACCGCACUUUGCAGAAACGAUAAGUUUAAGUAUAAGGUGUGAACUCUGCUCCAUGCUUAAUAUAU AAAUAAAAUUAUUUAACGAUAAUUUAAUUAAAUUUAGGUAAAUAGCAGCCUUAUUAUGAGGGUUAUAAUGUAGCGAAAUU CCUUGGCCUAUAAUUGAGGUCCCGCAUGAAUGACGUAAUGAUACAACAACUGUCUCCCCUUUAAGCUAAGUGAAAUUGAA AUCGUAGUGAAGAUGCUAUGUACCUUCAGCAAGACGGAAAGACCCUAUGCAGCUUUACUGUAAUUAGAUAGAUCGAAUUA UUGUUUAUUAUAUUCAGCAUAUUAAGUAAUCCUAUUAUUAGGUAAUCGUUUAGAUAUUAAUGAGAUACUUAUUAUAAUAU AAUGAUAAUUCUAAUCUUAUAAAUAAUUAUUAUUAUUAUUAUUAAUAAUAAUAAUAUGCUUUCAAGCAUAGUGAUAAAAC AUAUUUAUAUGAUAAUCACUUUACUUAAUAGAUAUAAUUCUUAAGUAAUAUAUAAUAUAUAUUUUAUAUAUAUUAUAUAU AAUAUAAGAGACAAUCUCUAAUUGGUAGUUUUGAUGGGGCGUCAUUAUCAGCAAAAGUAUCUGAAUAAGUCCAUAAAUAA AUAUAUAAAAUUAUUGAAUAAAAAAAAAAUAAUAUAUAUUAUAUAUAUUAAUUAUAAAUUGAAAUAUGUUUAUAUAAAUU UAUAUUUAUUGAAUAUAUUUUAGUAAUAGAUAAAAAUAUGUACAGUAAAAUUGUAAGGAAAACAAUAAUAACUUUCUCCU CUCUCGGUGGGGGUUCACACCUAUUUUUAAUAGGUGUGAACCCCUCUUCGGGGUUCCGGUUCCCUUUCGGGUCCCGGAAC UUAAAUAAAAAUGGAAAGAAUUAAAUUAAUAUAAUGGUAUAACUGUGCGAUAAUUGUAACACAAACGAGUGAAACAAGUA CGUAAGUAUGGCAUAAUGAACAAAUAACACUGAUUGUAAAGGUUAUUGAUAACGAAUAAAAGUUACGCUAGGGAUAACAG GGUAAUAUAGCGAAAGAGUAGAUAUUGUAAGCUAUGUUUGCCACCUCGAUGUCGACUCAACAUUUCCUCUUGGUUGUAAA AGCUAAGAAGGGUUUGACUGUUCGUCAAUUAAAAUGUUACGUGAGUUGGGUUAAAUACGAUGUGAAUCAGUAUGGUUCCU AUCUGCUGAAGGAAAUAUUAUCAAAUUAAAUCUCAUUAUUAGUACGCAAGGACCAUAAUGAAUCAACCCAUGGUGUAUCU AUUGAUAAUAAUAUAAUAUAUUUAAUAAAAAUAAUACUUUAUUAAUAUAUUAUCUAUAUUAGUUUAUAUUUUAAUUAUAU AUUAUCAUAGUAGAUAAGCUAAGUUGAUAAUAAAUAAAUAUUGAAUACAUAUUAAAUAUGAAGUUGUUUUAAUAAGAUAA UUAAUCUGAUAAUUUUAUACUAAAAUUAAUAAUUAUAGGUUUUAUAUAUUAUUUAUAAAUAAAUAUAUUAUAAUAAUAAU AAUUAUUAUUAUUAAUAAAAAAUAUUAAUUAUAAUAUUAAUAAAAUACUAAUUUAUCAGUUAUCUAUAUAAUAUCUAAUC UAUUAUUCUAUAUACU Large ribosomal subunit protein uL2m Saccharomyces cerevisiae W303 0.043870023 1 LEVO MLVLGSLRSALSCSSTASLISKRNPCYPYGILCRTLSQSVKLWQENTSKDDSSLNITPRLLKIIPNDTDIVTLEKQDELI KRRRKLSKEVTQMKRLKPVSPGLRWYRSPIYPYLYKGRPVRALTVVRKKHGGRNNSGKITVRHQGGGHRNRTRLIDFNRW EGGAQTVQRIEYDPGRSSHIALLKHNTTGELSYIIACDGLRPGDVVESFRRGIPQTLLNEMGGKVDPAILSVKTTQRGNC LPISMIPIGTIIHNVGITPVGPGKFCRSAGTYARVLAKLPEKKKAIVRLQSGEHRYVSLEAVATIGVVSNIDHQNRSLGK AGRSRWLGIRPTVRGVAMNKCDHPHGGGRGKSKSNKLSMSPWGQLAKGYKTRRGKNQNRMKVKDRPRGKDARL Large ribosomal subunit protein uL3m Saccharomyces cerevisiae W303 0.029843546999999998 1 LEVO MSKFLQGSIFSISKLHVRYSSTRPFLVAPSIANSITTEAPAINHSPELANARKWLPKRCGLITRKKGMMPYFDKSTGERS AATILEVNNVEVIMHRTSEVNGYFACQVGYGSRHLSKVSRQMLGHFASKVVNPKEHVAEFRVKDEKGLIPPGTLLKPSFF KEGQYVDVRSVSKGKGFTGVMKRYGFKGLRASHGTSIMHRHGGSYGQNQDPGRVLPGRKMPGHMGNEHVTIQNVKVLKVD DENNVIWVKGSVAGPKNSFVKIQDAIKKT Large ribosomal subunit protein uL4m Saccharomyces cerevisiae W303 0.032014609 1 LEVO MTIKRNLVKTLQSIRYQATTATAHAESTLNPLPNAAIPPKYALVTVRSFPSLEPLTFVPVPTSTVAAPLRRDILWRAVVY ENDNRRVGASNPPGRSENGFSRRKLMPQKGSGRARVGDANSPTRHNGGRALARTAPNDYTTELPSKVYSMAFNNALSHQY KSGKLFVIGGEKVDLISPTPELDLNRLDLVNTNTVEGKEIFEGEVIFRKFLEEFQLKGKRLLFITDKTREGLIKSSDPYK QKVDVIQKELVEVNDILRAQAVFIELEALEYLAMAHQKEILHSVSN Large ribosomal subunit protein uL5m Saccharomyces cerevisiae W303 0.033152031 1 LEVO MQRFSLVTHRSFSHSCVKPKSACSLVKPVHHLVKIDKSKLSPRFPELKYDKSDIRSPGFKPKDTHADRLNDHYLNTLQSD LLLINYSHNAAVVKGLKQRAWSGDSPYHLNRPPKNPRGSKAQLPDIHPIKWSNIPGLESVVINCFVREARENQLLAITAA LQLQQITGCKPHPIFSKNDVPTWKLRKGHQMGAKVELKGKEMSQFLSTLTEIVLPRIREYKGISNQSGNRFGGISFGLTA EDIKFFPEIDANQDSWPKTFGMHININTSAQLDYQARTLLSGFQFPFFGEEK Large ribosomal subunit protein uL6m Saccharomyces cerevisiae W303 0.023926886999999997 1 LEVO MSFIQRRLLSQTLFLRSQVGSLPLYISPEVQVSINALSMPRIIRKGRTSMNISQNITVKGPKGELSVEVPDFLHLDKDEK HGKINVTVQNSEDKHQRSMWGTVRSLINNHIIGVTEGHLAVLRFVGTGYRAQLENDGKFVNVKVGASIKQGLDVPEGIVV KTPAPTSLIIEGCNKQQVLLFAAKLRKFHPPEPYKGKGIYVNDETIKLKDKKIK Large ribosomal subunit protein bL9m Saccharomyces cerevisiae W303 0.016313828 1 LEVO MLHCTQVCLSALTKRTHRVKVQVLKDFPRFQLYKGQVANVKPSLMRNYLHNFNGAKYILSEEHDINTELLKQYQTLEAKL EEDHQQLSKRHETEVQKNMELRKESVFGHKKEEKPKEEKKGLLDSGITIEEVKIPGLDI Large ribosomal subunit protein uL13m Saccharomyces cerevisiae W303 0.0184995 1 LEVO MSQKIGHSGLAFARLWHHVDVARDKRTLGRLASAIAITLIGRHKPVYHPSQDCGDYVVVTNCQKIRVTGKKFEQKTYWSH SGRPGQLKLQTMNKVVADKGFGEILKKAVSGMLPKNKLRKQRLDRLKVFDGSENPYKQNITAFAHEQSSIPEPLKESIFN QLK Large ribosomal subunit protein uL14m Saccharomyces cerevisiae W303 0.014929664 1 LEVO MIFLKSVIKVIDNSGAQLAECIKVIRKGSPKSPAMVGDRIVCVIQKAKPLTQNITGTANTNRVKKGDICHAIVVRSKQRN MCRKDGSTVAFGDTACVLINKNTGEPLGTRIMANDGCVDRTLKDKGYNKICSLASRVI Large ribosomal subunit protein uL15m Saccharomyces cerevisiae W303 0.036414527 1 LEVO MKAERQTGLRNSFTTVIGRKLINTFVPSMMLTSVAGNDIFFRGLFKSPVLAFQSYRYVSILGQLKPSDGSTKSFKRLGRG PSSGLGKTSGRGQKGQKARGKVKSWFEGGQTPIYKLFPKIGFTNVGAKPLKELNLKRIQWFHDKNRLHLQPGEVLDMNKM RKLGLVTGPIKYGVKILASGKFHYNLPIALEASRASAKAIAAIEKAGGKFTARYYTPLGLRAHLNPQWFLEKRGRVPLQA RPTKRRDIDFYSKEEKRGYLVMEKDKLLQDIKEAQNKGSRHFLKQNVKKSSLEIELEELSPEKDWVPVVSNSKVMNIKAL DH Large ribosomal subunit protein uL16m Saccharomyces cerevisiae W303 0.026569025 1 LEVO MFPYLTRMNLSIKMGGLTLKESSPNAFLNNTTIARRFKHEYAPRFKIVQKKQKGRVPVRTGGSIKGSTLQFGKYGLRLKS EGIRISAQQLKEADNAIMRYVRPLNNGHLWRRLCTNVAVCIKGNETRMGKGKGGFDHWMVRVPTGKILFEINGDDLHEKV AREAFRKAGTKLPGVYEFVSLDSLVRVGLHSFKNPKDDPVKNFYDENAKKPSKKYLNILKSQEPQYKLFRGR Large ribosomal subunit protein bL17m Saccharomyces cerevisiae W303 0.026989383 1 LEVO MTVGIARKLSRDKAHRDALLKNLACQLFQHESIVSTHAKCKEASRVAERIITWTKRAITTSNSVAQAELKSQIQSQLFLA GDNRKLMKRLFSEIAPRYLERPGGYTRVLRLEPRANDSAPQSVLELVDSPVMSESHTVNRGNLKMWLLVKSVINDDANQL PHNPLTLQNLHKVAKFKAEAQLHGEIMLIKQVLLKEMSLPYDEALENERTQALLKEVYSSSLPKKTKKPSSYVMVPRP Large ribosomal subunit protein bL19m Saccharomyces cerevisiae W303 0.019428787 1 LEVO MWSRNVRLLGSWTRSYMVPATKRKTIPVYPPVQRIASSQIMKQVALSEIESLDPGAVKRKLISKKNKDRLKAGDVVRIVY DSSKCSYDTFVGYILSIDRKQLVQDASLLLRNQIAKTAVEIRVPLFSPLIERIDLLTPHVSSRQRNKHYYIRGTRLDVGD LEAGLRRKK Large ribosomal subunit protein bL21m Saccharomyces cerevisiae W303 0.018420539 1 LEVO MLQLKFIWPVARITPIYRPFTSHPFRNLATSSSISSTKAKTTKTDTTPLKLSNELYAIFKIHNRPYLVTEGDRVILPFKL KQAEVGDILNMTDVTTLGSRNYKLVGHPINTSLYTLKATVVGKTKRAFQTREVTKRRNRRVRHAKSKGDLTILRISELSM N Large ribosomal subunit protein uL22m Saccharomyces cerevisiae W303 0.034957363 1 LEVO MNFHTARISQVGVISRALLSSVSRRWIHVTPISLNNSGGSLFGSITENKPKEGKNRGDEDAGSFSNRLAIASDSSGEAPE VNRDSITIENDKLLQQHIISLQQPEQLASQSLLSPLKREIYEANCKINGGFYKKDTIVKLPNSSERYKLKLTKREIEVLE PSVYAQSYRIKSSMKKATLLLRLLGGLDVMKAISQCHFSNKKIAREVAELLQKGVKDGQKLGLKPEDLYISQIWTGSDGF WRKRVEFKARTRIGIISHPYIHVRCILRTKSVTKRRLAYEAHLKEQKRAPWVQLGDKPIRGVTGGVYKW Large ribosomal subunit protein uL23m Saccharomyces cerevisiae W303 0.030616221 1 LEVO MPRLTVGTKNMLYPLQKTLAVGSCKPEQVPIRSLASVVESSSKILDKSGSDREVDINVSEKIYKWTKAGIEQGKEHFKVG GNKVYFPKARIILLRPNAKHTPYQAKFIVPKSFNKLDLRDYLYHIYGLRAMNITTQLLHGKFNRMNLQTTRFREPQIKKM TIEMEEPFIWPEEPRPDENSFWDSTTPDNMEKYREERLNCLGSDANKPGTAFDGVVGPYERVAQPFIPRFLKREIDNKRE RHAAELQRADKLIALNRYIEDLH Large ribosomal subunit protein uL24m Saccharomyces cerevisiae W303 0.033808039 1 LEVO MSGSYQHLSNVGSRVMKRLGNRPKNFLPHSEKFIKKSTPEFMKSDLKEVDEKTSFKSEKEWKFIPGDRVVVMSGASKGNI AVIKSFDKRTNSFILDENGPTKTVPVPKQFWLEGQTSHMITIPVSILGKDLRLVADIDDEKTPGKTRTVAVRDVSFNGSY YDADYKKVMPYRCVKGQPDLIIPWPKPDPIDVQTNLATDPVIAREQTFWVDSVVRNPIPKKAIPSIRNPHSKYKRGTLTA KDIAKLVAPEMPLTEVRKSHLAEKKELAEREVPKLTEEDMEAIGARVFEFLEKQKRE Large ribosomal subunit protein bL27m Saccharomyces cerevisiae W303 0.043310684 1 LEVO MWNPILLDTSSFSFQKHVSGVFLQVRNATKRAAGSRTSMKDSAGRRLGPKKYEGQDVSTGEIIMRQRGTKFYPGENVGIG KDHSIFALEPGVVRYYLDPFHPKRKFIGVALRRDLKLPSPHFEPTVRRFGRFELTNKRAAYKEENSISRKDYLAKPNILK QLEVRESKRKELQDKLSKVLRDELKLDIKDIELATSYLIRVRASLKNGYPIEDARFNSRYYLKEEERLKARRESWTNEKL SESLSKIDECSDLLNSSTSFNNKLELHQYISEQEKQALKAKLLEDLEKSQHLETKKDKNYIKALFKDACNFLTLSEEVHL RRKYLKSVFPETDSTVETKSGKKSIVSRRFDYTKNKVEVIARSRRAFLSKL Large ribosomal subunit protein bL28m Saccharomyces cerevisiae W303 0.030109249999999997 1 LEVO MQKIFRPFQLTRGFTSSVKNFRQWRLIETRKIAKQPNYQVGDAKPLHMPKERKKFPDYKYGESNIFKQSNKGLYGGSFVQ FGNNISESKAKTRKKWLPNVVKKGLWSETLNRKISIKMTAKVLKTISKEGGIDNYLTKEKSARIKELGPTGWKLRYRVLK RKDEIENPPHKDAPIIEMAGGKKAKIYYDEIVNGSPRKISVGRRRLMSFLYPLEKLEYRSVGKDLNYKKFVELFADVPVK DILARLEDHKFDLSTITV Large ribosomal subunit protein uL29m Saccharomyces cerevisiae W303 0.037019922 1 LEVO MWKRSFHSQGGPLRARTKFTKPKPKQPVLPKDKIRPPTQLTHHSNNLRITEPIPPTTSNLRCPDDHPLWQFFSNKKFIRS ADDLPPSSHIRPWSIPELRHKSFNDLHSLWYNCLREQNVLARENHLLKNIVGSTHDEFSELSNSIRTTMWQIRHVLNERE LAYSASREFLQDESERKKFLDTLANDYFLNKDIPDDEVASMLTRFQLAIFGISETIQDNTVDINFIDGIKFLANLKLQRF KDSNDLISEISQEPITDVGESFILFTSDFEPHAVQEACVAIKDLRKSPDNKVPKLDELPTVRKYLKQLIHASSVEQATA Large ribosomal subunit protein uL30m Saccharomyces cerevisiae W303 0.009549273999999998 1 LEVO MVFYKVTLSRSLIGVPHTTKSIVKSLGLGKRGSIVYKKVNPAIAGSLAKVKELVKVEVTEHELTPSQQRELRKSNPGFIV EKRTID Small ribosomal subunit protein mS26 Saccharomyces cerevisiae W303 0.036059281 1 LEVO MGKGAAKYGFKSGVFPTTRSILKSPTTKQTDIINKVKSPKPKGVLGIGYAKGVKHPKGSHRLSPKVNFIDVDNLIAKTVA EPQSIKSSNGSAQKVRLQKAELRRKFLIEAFRKEEARLLHKHEYLQKRTKELEKAKELELEKLNKEKSSDLTIMTLDKMM SQPLLRNRSPEESELLKLKRNYNRSLLNFQAHKKKLNELLNLYHVANEFIVTESQLLKKIDKVFNDETEEFTDAYDVTSN FTQFGNRKLLLSGNTTLQTQINNAIMGSLSNEKFFDISLVDSYLNKDLKNISNKIDSKLNPTSNGAGNNGNNNNTTNL Large ribosomal subunit protein bL31m Saccharomyces cerevisiae W303 0.020125756 1 LEVO MLKSIFAKRFASTGSYPGSTRITLPRRPAKKIQLGKSRPAIYHQFNVKMELSDGSVVIRRSQYPKGEIRLIQDQRNNPLW NPSRDDLVVVDANSGGSLDRFNKRYSSLFSVDSTTPNSSSETVELSEENKKKTQIKKEEKEDVSEKAFGMDDYLSLLDDS EQQIKSGKLASKKRDKK Large ribosomal subunit protein bL32m Saccharomyces cerevisiae W303 0.021483059 1 LEVO MNSLIFGKQLAFHKIVPTTAIGWLVPLGNPSLQIPGQKQLGSIHRWLREKLQQDHKDTEDKDFFSNNGILLAVPKKKVSH QKKRQKLYGPGKKQLKMIHHLNKCPSCGHYKRANTLCMYCVGQISHIWKTHTAKEEIKPRQEEELSELDQRVLYPGRRDT KYTKDLKDKDNYLERRVRTLKKD Large ribosomal subunit protein bL33m Saccharomyces cerevisiae W303 0.007992649999999999 1 LEVO MVKVKSKNSVIKLLSTAASGYSRYISIKKGAPLVTQVRYDPVVKRHVLFKEAKKRKVAERKPLDFLRTAK Large ribosomal subunit protein bL34m Saccharomyces cerevisiae W303 0.012054304 1 LEVO MPLFARLCQPQSRRMFSSISSFSALSVLRPQTGMLLNSSPLKTPSFTPLGFGLIGQRRWKSRGNTYQPSTLKRKRTFGFL ARAKSKQGSKILKRRKLKGRWFLSH Large ribosomal subunit protein bL35m Saccharomyces cerevisiae W303 0.013276666999999999 1 LEVO MKISLHNKRQRGDQNQNMSVFNVLKPLLKGSNSFKVKLNGFLFNNVSTITIRTLMKTHKGTAKRWRRTGNTFKRGIAGRK HGNIGWSHRSLKALTGRKIAHPAYSKHLKRLLPYH Large ribosomal subunit protein mL58 Saccharomyces cerevisiae W303 0.022431871 1 LEVO MIGRGVCCRSFHTAGSAWKQFGFPKTQVTTIYNKTKSASNYKGYLKHRDAPGMYYQPSESIATGSVNSETIPRSFMAASD PRRGLDMPVQSTKAKQCPNVLVGKSTVNGKTYHLGPQEIDEIRKLRLDNPQKYTRKFLAAKYGISPLFVSMVSKPSEQHV QIMESRLQEIQSRWKEKRRIAREDRKRRKLLWYQA Large ribosomal subunit protein mL59 Saccharomyces cerevisiae W303 0.018626977 1 LEVO MSYKQYFDSLPLKLKSFFQRYPPSIKYSPVSTSTKAINANPFLPNKHPVTQRFHDPKYSLRRMSDVYKLALRYGVEEFLP PIENTKKLFFEEKYNKKTLMKGVLLPKGHKHELKLNEKLKKREEALKKVDELIASKKGSKYAKRVEKMKKNQSIGWF Large ribosomal subunit protein mL60 Saccharomyces cerevisiae W303 0.015555466 1 LEVO MFGPFKLTSPVAGGLLWKIPWRMSTHQKTRQRERLRNVDQVIKQLTLGLHVQRCQDKGLTYQEAMESKKKYKPRSKSLRL LNKPSVFPKENQMSSKDKYWTFDKKAVGYRKGIHKVPKWTKISIRKAPKFF Large ribosomal subunit protein mL67 Saccharomyces cerevisiae W303 0.026937761999999997 1 LEVO MKVNHSISRFRPASWFEKTKIIPPQVYIFRNLEYGQVLYSQFPNFSQTQVDKLFVRPNWSNRKPSLRRDIWKCMCVVNLQ NYKQSVHLYQNLCRLRYLRDVAQRKESDKLRKKDSNGHVWYSGQYRPTYCQEAVADLRESLLKVFENATPAEKQTVPAKK PSIYWEDPWRMGDKDKHWNYDVFNALGLEHKLIQRVGNIAREESVILKELAKLESHPTEQTEVSSQ 15S mitochondrial rRNA Saccharomyces cerevisiae W303 0.5287841249999999 1 GUAAAAAAUUUAUAAGAAUAUGAUGUUGGUUCAGAUUAAGCGCUAAAUAAGGACAUGACACAUGCGAAUCAUACGUUUAU UAUUGAUAAGAUAAUAAAUAUGUGGUGUAAACGUGAGUAAUUUUAUUAGGAAUUAAUGAACUAUAGAAUAAGCUAAAUAC UUAAUAUAUUAUUAUAUAAAAAUAAUUUAUAUAAUAAAAAGGAUAUAUAUAUAAUAUAUAUUUAUCUAUAGUCAAGCCAA UAAUGGUUUAGGUAGUAGGUUUAUUAAGAGUUAAACCUAGCCAACGAUCCAUAAUCGAUAAUGAAAGUUAGAACGAUCAC GUUGACUCUGAAAUAUAGUCAAUAUCUAUAAGAUACAGCAGUGAGGAAUAUUGGACAAUGAUCGAAAGAUUGAUCCAGUU ACUUAUUAGGAUGAUAUAUAAAAAUAUUUUAUUUUAUUUAUAAAUAUUAAAUAUUUAUAAUAAUAAUAAUAAUAAUAUAU AUAUAUAAAUUGAUUAAAAAUAAAAUCCAUAAAUAAUUAAAAUAAUGAUAUUAAUUACCAUAUAUAUUUUUAUAUGGAUA UAUAUAUUAAUAAUAAUAUUAAUUUUAUUAUUAUUAAUAAUAUAUUUUAAUAGUCCUGACUAAUAUUUGUGCCAGCAGUC GCGGUAACACAAAGAGGGCGAGCGUUAAUCAUAAUGGUUUAAAGGAUCCGUAGAAUGAAUUAUAUAUUAUAAUUUAGAGU UAAUAAAAUAUAAUUAAAGAAUUAUAAUAGUAAAGAUGAAAUAAUAAUAAUAAUUAUAAGACUAAUAUAUGUGAAAAUAU UAAUUAAAUAUUAACUGACAUUGAGGGAUUAAAACUAGAGUAGCGAAACGGAUUCGAUACCCGUGUAGUUCUAGUAGUAA ACUAUGAAUACAAUUAUUUAUAAUAUAUAUUAUAUAUAAAUAAUAAAUGAAAAUGAAAGUAUUCCACCUGAAGAGUACGU UAGCAAUAAUGAAACUCAAAACAAUAGACGGUUACAGACUUAAGCAGUGGAGCAUGUUAUUUAAUUCGAUAAUCCACGAC UAACCUUACCAUAUUUUGAAUAUUAUAAUAAUUAUUAUAAUUAUUAUAUUACAGGCGUUACAUUGUUGUCUUUAGUUCGU GCUGCAAAGUUUUAGAUUAAGUUCAUAAACGAACAAAACUCCAUAUAUAUAAUUUUAAUUAUAUAUAAUUUUAUAUUAUU UAUUAAUAUAAAGAAAGGAAUUAAGACAAAUCAUAAUGAUCCUUAUAAUAUGGGUAAUAGACGUGCUAUAAUAAAAUGAU AAUAAAAUUAUAUAAAAUAUAUUUAAUUAUAUUUAAUUAAUAAUAUAAAACAUUUUAAUUUUUAAUAUAUUUUUUUAUUA UAUAUUAAUAUGAAUUAUAAUCUGAAAUUCGAUUAUAUGAAAAAAGAAUUGCUAGUAAUACGUAAAUUAGUAUGUUACGG UGAAUAUUCUAACUGUUUCGCACUAAUCACUCAUCACGCGUUGAAACAUAUUAUUAUCUUAUUAUUUAUAUAAUAUUUUU UAAUAAAUAUUAAUAAUUAUUAAUUUAUAUUUAUUUAUAUCAGAAAUAAUAUGAAUUAAUGCGAAGUUGAAAUACAGUUA CCGUAGGGGAACCUGCGGUGGGCUUAUAAAUAUCUUAAAUAUUCUUACA MAGNESIUM ION 2.4305e-05 299 MG GUANOSINE-5'-TRIPHOSPHATE 0.0005231799999999999 1 GTP singleParticle particle 7.4 Quantifoil R2/2 COPPER 300 CARBON HOLEY CARBON CONTINUOUS 200.0 GLOW DISCHARGE ETHANE 100 277 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.4 2.6 165000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K3 (6k x 4k) 38.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 3.2 FSC 0.143 CUT-OFF cryoSPARC 22558 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD 5MRC PDB experimental model

Large subunit and E-site tRNA

8OM4 PDB experimental model

Small subunit

AlphaFold in silico model

Aep1, Aep2, Atp25