2025-10-22 REL PDBe PDBe PDBe 2025-01-28 2025-08-13 2025-08-13 2025-10-22 Swiss National Science Foundation 310030_207453 Switzerland Swiss National Science Foundation 310030_197776 Switzerland MEF in-situ nucleosome consensus structure Eibauer M Medalia O MEF, nucleosome, NUCLEAR PROTEIN Wang B Kronenberg-Tenga R Rosti V Di Patrizio Soldateschi E Luo Q Iannacchero UM Pinet L Eibauer M Boujemaa-Paterski R Schuler B Lanzuolo C Medalia O The molecular basis of lamin-specific chromatin interactions. Nat.Struct.Mol.Biol. US 32 1999 2011 2025 40750945 doi:10.1038/s41594-025-01622-5 1545-9985 EMDB EMD-52630 associated EM volume Native nucleosome of mouse embryonic fibroblasts Native nucleosome of mouse embryonic fibroblasts 0 Mus musculus embryonic fibroblast nucleus nucleus 0.2 subtomogramAveraging cell 7.4 1.0 PBS Quantifoil R2/1 GOLD CARBON HOLEY GLOW DISCHARGE OTHER ETHANE HOMEMADE PLUNGER TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 4.0 4.0 64000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 2.2 4.0 1 1 C1 FOURIER SPACE 15.0 FSC 0.143 CUT-OFF RELION 5.0-beta3
Reference model EMD-33132, low-pass filtered to 40 A
103173 43 130000 crYOLO 1.3 IMOD RELION 5.0-beta3 PHASE FLIPPING AND AMPLITUDE CORRECTION MAXIMUM LIKELIHOOD RELION 5.0-beta3 emd_52630.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 64 64 64 0 0 0 64 64 64 282.88 282.88 282.88 90.0 90.0 90.0 X Y Z -0.7330949 1.5427719 0.009685847 0.1484882 4.42 4.42 4.42 0.8 AUTHOR MEF in-situ nucleosome consensus structure emd_52630_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 64 64 64 0 0 0 64 64 64 282.88 282.88 282.88 90.0 90.0 90.0 X Y Z -0.71271104 1.5188303 0.009504291 0.1467179 4.42 4.42 4.42 AUTHOR half2_zflip emd_52630_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 64 64 64 0 0 0 64 64 64 282.88 282.88 282.88 90.0 90.0 90.0 X Y Z -0.68207496 1.4126571 0.009867403 0.14726949 4.42 4.42 4.42 AUTHOR half1_zflip