2026-03-11 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_PubMed _citation.title _citation_author.identifier_ORCID _citation_author.name _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation.pdbx_database_id_PubMed _citation.title _citation_author.identifier_ORCID _citation_author.name _em_admin.last_update PDBe PDBe 2025-01-28 2026-02-25 2026-02-25 2026-03-11 Novo Nordisk Foundation NNF21OC0070452 Denmark Single particle cryo electron microscopy of a Fab fragment bound to recombinant human CD40 ligand Kristoffersen EL Schinkel T Andersen ES CD40 ligand inhibitor, CD154 inhibitor, autoimmunity, Fab fragment, IMMUNE SYSTEM Pedersen K Green K Kristoffersen EL Schinkel T Hansen AG Palarasah Y Rovsing AB Andersen ES Valero J Civit L Laursen NS Troldborg A Degn SE Thiel S Curbing Autoimmunity: A New Fab Fragment Targeting CD40-CD40L Halts B-Cell Activation and Differentiation. Eur.J.Immunol. GW 56 e70158 e70158 2026 41742604 doi:10.1002/eji.70158 0014-2980 0794 EJIMAF 9i5n FULLOVERLAP EMDB EMD-52634 associated EM volume
Single particle cryo electron microscopy of a Fab fragment bound to recombinant human CD40 ligand
 Incubated sample containing Fab fragment and recombinant human CD40 ligand. Incubated sample containing Fab fragment and recombinant human CD40 ligand. 0 1 2 3
Buffer: 1x PBS, 1 mM MgCl2; 1:3 molar ratio, 2.47 uM rhCD40L and 7.42 uM Fab20
Mus musculus Fab20 heavy chain Mus musculus 0.012772167
Fab fragment heavy chain, only the variable region
1 Homo sapiens LEVO QVQLKQSGPGLVQPSQSLSITCTVSGFSLTGYGVHWVRQSPGKGLEWLGMIWSGGSTDYNAAFISRLSISKDSSKSQVFF KMNSLQANDTAIYYCARSSGNYGEDAMDYWGQGTSVTV Fab20 light chain Mus musculus 0.011825098
Fab fragment light chain, only the variable region
1 Homo sapiens LEVO ETTVTQSPASLSMAIGEKVTIRCITSTDIDDDMNWYQQKPGEPPELLISEDNTLRPGVPSRFSSSGYGTDFVFAIENMLP EDVADYFCLQSDNLPFTFGSGTKLEIK CD40 ligand, soluble form Homo sapiens 0.015512547
One subunit of extracellular domain of trimeric hCD40L
1 Escherichia coli BL21(DE3) LEVO NPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSP GRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL P29965 MAGNESIUM ION 2.4305e-05 1 MG water 1.8015e-05 13 HOH singleParticle particle 7.5 1.0 PBS Phosphate-buffered saline 1.0 MgCl2 magnesium chloride Au-flat 1.2/1.3 GOLD 300 GLOW DISCHARGE OTHER 0.015 ETHANE 99 277 LEICA EM GP TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 0.8 1.8 130000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Bioquantum 20 GATAN K3 BIOQUANTUM (6k x 4k) 2 5673 1.5 60.0
1680 movies recorded from one grid and 3993 movies recorded from other grid. After curation 4307 were used.
 1 cryoSPARC 4.6.0 PHASE FLIPPING AND AMPLITUDE CORRECTION 3.4 FSC 0.143 CUT-OFF 311649 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_52634.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 388.2 388.2 388.2 90.0 90.0 90.0 X Y Z -1.696242 2.8209622 -0.0000765345 0.025903292 1.294 1.294 1.294 0.4 AUTHOR Symmetry expanded focus map (sharpened) of CD40 ligand with Fab fragment bound emd_52634_msk_1.map emd_52634_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 388.2 388.2 388.2 90.0 90.0 90.0 X Y Z -0.9511652 1.6708785 0.0001613578 0.03642715 1.294 1.294 1.294 AUTHOR Symmetry expanded focus map (half map B) of CD40 ligand with Fab fragment bound emd_52634_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 388.2 388.2 388.2 90.0 90.0 90.0 X Y Z -0.9135591 1.5287786 0.0001776388 0.03685279 1.294 1.294 1.294 AUTHOR Symmetry expanded focus map (half map A) of CD40 ligand with Fab fragment bound