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    <admin>
        <current_status>
            <date>2025-09-10</date>
            <code>REL</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2025-02-05</deposition>
            <header_release>2025-09-10</header_release>
            <map_release>2025-09-10</map_release>
            <update>2025-09-10</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>Swedish Research Council</funding_body>
                <code>2022-03681</code>
                <country>Sweden</country>
            </grant_reference>
            <grant_reference>
                <funding_body>Novo Nordisk Foundation</funding_body>
                <code>NNF20OC0064789</code>
                <country>Denmark</country>
            </grant_reference>
        </grant_support>
        <title>L-PTC from Clostridium botulinum serotype B1, focused map of HA17-HA33 subcomplex (leg 2)</title>
        <authors_list>
            <author ORCID="0009-0007-5842-8527">Krc A</author>
            <author ORCID="0000-0001-7893-0249">Persson Kosenina S</author>
            <author ORCID="0000-0002-9527-2310">Masuyer G</author>
            <author ORCID="0000-0003-4777-3417">Stenmark P</author>
        </authors_list>
        <keywords>progenitor-toxin complex, botulinum neurotoxin, hemagglutinin, TOXIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author ORCID="0009-0007-5842-8527" order="1">Krc A</author>
                    <author ORCID="0000-0001-7893-0249" order="2">Kosenina SP</author>
                    <author ORCID="0000-0003-2592-2442" order="3">Nowakowska MB</author>
                    <author ORCID="0000-0002-9527-2310" order="4">Masuyer G</author>
                    <author ORCID="0000-0003-4777-3417" order="5">Stenmark P</author>
                    <title>Structure of the complete 14-subunit botulinum neurotoxin B complex reveals a unique anchoring through the narrow central pore of HA70.</title>
                    <journal_abbreviation>Sci Adv</journal_abbreviation>
                    <country>US</country>
                    <volume>11</volume>
                    <first_page>eadx5058</first_page>
                    <last_page>eadx5058</last_page>
                    <year>2025</year>
                    <external_references type="PUBMED">40864696</external_references>
                    <external_references type="DOI">doi:10.1126/sciadv.adx5058</external_references>
                    <external_references type="ISSN">2375-2548</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <other_db_list>
            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-52740</accession_id>
                <content_type>associated EM volume</content_type>
            </db_reference>
        </other_db_list>
    </crossreferences>
    <sample>
        <name>HA17-HA33 subcomplex from Clostridium botulinum serotype B1</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>HA17-HA33 subcomplex from Clostridium botulinum serotype B1</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="1491">Clostridium botulinum</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.21</theoretical>
                </molecular_weight>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Hemagglutinin component HA17</name>
                <natural_source database="NCBI">
                    <organism ncbi="1491">Clostridium botulinum</organism>
                </natural_source>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MSAERTFLPNGNYNIKSIFSGSLYLSPVSGSLTFSNESSANNQKWNVEYMAENRCFKISNVAEPNKYLSYDNFGFISLDSLSNRCYWFPIKIAVNTYIMLSLNKVNELDYAWDIYDTNENILSQPLLLLPNFDIYNSNQMFKLEKI</string>
                    <external_references type="UNIPROTKB">B1INP9</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Hemagglutinin component HA33</name>
                <natural_source database="NCBI">
                    <organism ncbi="1491">Clostridium botulinum</organism>
                </natural_source>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MEHYSTIQNSLNDKIVTISCKANTDLFFYQVPGNGNVSLFQQTRNYLERWRIIYDSNKAAYKIKSMNIYNTNLVLTWNAPTHNISAQQDSNADNQYWLLLKDIGNNSFIIASYKNPNLVLYADTVARNLKLSTLNNSSYIKFIIEDYVISDFKNFTCRISPILAGGKVVQQVSMTNLAVNLYIWNNDLNQKWTIIYNEEKAAYQFFNKILSNGVLTWIFSDGNTVRVSSSAQNNDAQYWLINPVSDNYDRYTITNLRDKTKVLDLYGGQTADGTTIQVFNSNGGDNQIWTMSNP</string>
                    <external_references type="UNIPROTKB">B1INP8</external_references>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="3">
                <name>Hemagglutinin component HA33</name>
                <natural_source database="NCBI">
                    <organism ncbi="1491">Clostridium botulinum</organism>
                </natural_source>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                </recombinant_expression>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MEHYSTIQNSLNDKIVTISCKANTDLFFYQVPGNGNVSLFQQTRNYLERWRIIYDSNKAAYKIKSMNIYNTNLVLTWNAPTHNISAQQDSNADNQYWLLLKDIGNNSFIIASYKNPNLVLYADTVARNLKLSTLNNSSYIKFIIEDYVISDFKNFTCRISPILAGGKVVQQVSMTNLAVNLYIWNNDLNQKWTIIYNEEKAAYQFFNKILSNGVLTWIFSDGNTVRVSSSAQNNDAQYWLINPVSDNYDRYTITNLRDKTKVLDLYGGQTADGTTIQVFNSNGGDNQIWTMSNP</string>
                    <external_references type="UNIPROTKB">B1INP8</external_references>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
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        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
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                        <ph>7.5</ph>
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                        <model>Quantifoil R1.2/1.3</model>
                        <material>COPPER</material>
                        <mesh>300</mesh>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                            <film_topology>HOLEY</film_topology>
                        </support_film>
                        <pretreatment>
                            <type>GLOW DISCHARGE</type>
                            <time units="s">120</time>
                        </pretreatment>
                        <details>Graphene oxide coating</details>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
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                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">50.0</c2_aperture_diameter>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_defocus_min units="µm">0.6</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.4</nominal_defocus_max>
                    <nominal_magnification>105000.0</nominal_magnification>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <specialist_optics>
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                            <slit_width units="eV">20</slit_width>
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                            <film_or_detector_model>GATAN K3 (6k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">40.0</average_electron_dose_per_image>
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                    <type>PHASE FLIPPING AND AMPLITUDE CORRECTION</type>
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                <final_reconstruction>
                    <resolution units="Å" res_type="BY AUTHOR">3.38</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
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                    <type>PROJECTION MATCHING</type>
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                    <type>PROJECTION MATCHING</type>
                    <projection_matching_processing/>
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