2026-03-18 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY PDBe PDBe 2025-02-22 2026-02-18 2026-02-18 2026-03-18 Research Council of Lithuania S-MIP-19-32 Lithuania PAM-bound Cas9-Cas1-Cas2-Csn2 supercomplex in the locked conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system Sasnauskas G Gaizauskaite U Tamulaitiene G CIRSPR-Cas, spacer acquisition, Csn2, Cas1, Cas2, CRISPR integrase, DNA BINDING PROTEIN Gaizauskaite U Tamulaitiene G Silanskas A Gasiunas G Siksnys V Sasnauskas G Structural insights into Cas9-mediated prespacer selection in CRISPR-Cas adaptation. Mol.Cell US 86 791 804.e9 2026 41702403 doi:10.1016/j.molcel.2026.01.022 1097-2765 2168 MOCEFL EMDB EMD-52885 associated EM volume PAM-bound Cas9-Cas1-Cas2-Csn2 supercomplex in the locked conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system PAM-bound Cas9-Cas1-Cas2-Csn2 supercomplex in the locked conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system 0 1 2 3 4 5 6 7 8 Streptococcus thermophilus DGCC 7710 singleParticle particle 7.5 ETHANE FEI VITROBOT MARK IV TFS GLACIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 200 50.0 2.7 1.0 2.0 92000.0 NITROGEN FEI FALCON III (4k x 4k) COUNTING 46.33 30.0 1 cryoSPARC 4.5.3 PHASE FLIPPING AND AMPLITUDE CORRECTION cryoSPARC ab-initio C1 FOURIER SPACE 4.2 FSC 0.143 CUT-OFF cryoSPARC 4.6.2 24394 MAXIMUM LIKELIHOOD cryoSPARC 4.5.3 MAXIMUM LIKELIHOOD cryoSPARC 4.6.2 emd_52885.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 336 336 336 0 0 0 336 336 336 369.6 369.6 369.6 90.0 90.0 90.0 X Y Z -0.4841025 1.4286265 0.0005322988 0.049251083 1.1 1.1 1.1 0.3 AUTHOR Unsharpened focused map obtained with a mask covering Cas1 and Csn2 subunits 9h2g PDB experimental model emd_52885_msk_1.map emd_52885_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 336 336 336 0 0 0 336 336 336 369.6 369.6 369.6 90.0 90.0 90.0 X Y Z -1.032411 1.7886301 0.0019910722 0.12806009 1.1 1.1 1.1 AUTHOR Half map 2 emd_52885_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 336 336 336 0 0 0 336 336 336 369.6 369.6 369.6 90.0 90.0 90.0 X Y Z -0.99610233 1.8923303 0.0018682355 0.12649567 1.1 1.1 1.1 AUTHOR Half map 1