2026-03-18 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update EXPERIMENTAL_SUMMARY citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update DATABASE_REFERENCES citation em_admin _citation.journal_volume _citation.page_first _citation.page_last _em_admin.last_update EXPERIMENTAL_SUMMARY citation em_admin _citation.journal_volume _citation.page_first _citation.page_last _em_admin.last_update PDBe PDBe 2025-02-22 2026-02-18 2026-02-18 2026-03-18 Research Council of Lithuania S-MIP-19-32 Lithuania PAM-bound Cas9-Cas1-Cas2-Csn2 supercomplex in the locked conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system Sasnauskas G Gaizauskaite U Tamulaitiene G CIRSPR-Cas, spacer acquisition, Csn2, Cas1, Cas2, CRISPR integrase, DNA BINDING PROTEIN Gaizauskaite U Tamulaitiene G Silanskas A Gasiunas G Siksnys V Sasnauskas G Structural insights into Cas9-mediated prespacer selection in CRISPR-Cas adaptation. Mol.Cell US 86 791 804.e9 2026 41702403 doi:10.1016/j.molcel.2026.01.022 1097-2765 2168 MOCEFL EMD-52883 consensus EM volume
Consensus map
 EMD-52884 focused EM volume
Focused map 1, mask covers Cas9 and Cas1 subunits
 EMD-52885 focused EM volume
Focused map 2, mask covers Cas1 and Csn2 subunits
 9q85 FULLOVERLAP EMDB EMD-52883 consensus EM volume
Consensus map
 EMDB EMD-52884 focused EM volume
Focused map 1, mask covers Cas9 and Cas1 subunits
 EMDB EMD-52885 focused EM volume
Focused map 2, mask covers Cas1 and Csn2 subunits
 EMDB EMD-52886 associated EM volume
PAM-bound Cas9-Cas1-Cas2-Csn2 supercomplex in the locked conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system
 PAM-bound Cas9-Cas1-Cas2-Csn2 supercomplex in the locked conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system PAM-bound Cas9-Cas1-Cas2-Csn2 supercomplex in the locked conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system 0 1 2 3 4 5 6 7 8 Streptococcus thermophilus DGCC 7710 CRISPR-associated endonuclease Cas9 Streptococcus thermophilus DGCC 7710 0.16249853099999997 1 Escherichia coli BL21(DE3) LEVO MTKPYSIGLDIGTNSVGWAVITDNYKVPSKKMKVLGNTSKKYIKKNLLGVLLFDSGITAEGRRLKRTARRRYTRRRNRIL YLQEIFSTEMATLDDAFFQRLDDSFLVPDDKRDSKYPIFGNLVEEKVYHDEFPTIYHLRKYLADSTKKADLRLVYLALAH MIKYRGHFLIEGEFNSKNNDIQKNFQDFLDTYNAIFESDLSLENSKQLEEIVKDKISKLEKKDRILKLFPGEKNSGIFSE FLKLIVGNQADFRKCFNLDEKASLHFSKESYDEDLETLLGYIGDDYSDVFLKAKKLYDAILLSGFLTVTDNETEAPLSSA MIKRYNEHKEDLALLKEYIRNISLKTYNEVFKDDTKNGYAGYIDGKTNQEDFYVYLKNLLAEFEGADYFLEKIDREDFLR KQRTFDNGSIPYQIHLQEMRAILDKQAKFYPFLAKNKERIEKILTFRIPYYVGPLARGNSDFAWSIRKRNEKITPWNFED VIDKESSAEAFINRMTSFDLYLPEEKVLPKHSLLYETFNVYNELTKVRFIAESMRDYQFLDSKQKKDIVRLYFKDKRKVT DKDIIEYLHAIYGYDGIELKGIEKQFNSSLSTYHDLLNIINDKEFLDDSSNEAIIEEIIHTLTIFEDREMIKQRLSKFEN IFDKSVLKKLSRRHYTGWGKLSAKLINGIRDEKSGNTILDYLIDDGISNRNFMQLIHDDALSFKKKIQKAQIIGDEDKGN IKEVVKSLPGSPAIKKGILQSIKIVDELVKVMGGRKPESIVVEMARENQYTNQGKSNSQQRLKRLEKSLKELGSKILKEN IPAKLSKIDNNALQNDRLYLYYLQNGKDMYTGDDLDIDRLSNYDIDHIIPQAFLKDNSIDNKVLVSSASNRGKSDDFPSL EVVKKRKTFWYQLLKSKLISQRKFDNLTKAERGGLLPEDKAGFIQRQLVETRQITKHVARLLDEKFNNKKDENNRAVRTV KIITLKSTLVSQFRKDFELYKVREINDFHHAHDAYLNAVIASALLKKYPKLEPEFVYGDYPKYNSFRERKSATEKVYFYS NIMNIFKKSISLADGRVIERPLIEVNEETGESVWNKESDLATVRRVLSYPQVNVVKKVEEQNHGLDRGKPKGLFNANLSS KPKPNSNENLVGAKEYLDPKKYGGYAGISNSFAVLVKGTIEKGAKKKITNVLEFQGISILDRINYRKDKLNFLLEKGYKD IELIIELPKYSLFELSDGSRRMLASILSTNNKRGEIHKGNQIFLSQKFVKLLYHAKRISNTINENHRKYVENHKKEFEEL FYYILEFNENYVGAKKNGKLLNSAFQSWQNHSIDELCSSFIGPTGSERKGLFELTSRGSAADFEFLGVKIPRYRDYTPSS LLKDATLIHQSVTGLYETRIDLAKLGEGLEGHHHHHH G3ECR1 3.1.-.- crRNA synthetic construct 0.013609083 1 CGCUAAAGAGGAAGAGGACAGUUUUAGAGCUGUGUUGUUUCG tracrRNA synthetic construct 0.024119356999999998 1 CGAAACAACACAGCGAGUUAAAAUAAGGCUUAGUCCGUACUCAACUUGAAAAGGUGGCACCGAUUCGGUGUUUUU DNA (43-MER) synthetic construct 0.015003586 1 (DG)(DA)(DT)(DG)(DT)(DC)(DT)(DT)(DC)(DC)(DA)(DA)(DT)(DT)(DT)(DA)(DC)(DT)(DA)(DC) (DT)(DC)(DG)(DT)(DT)(DC)(DT)(DG)(DG)(DT)(DG)(DT)(DT)(DT)(DC)(DT)(DC)(DG)(DA)(DG) (DG)(DC)(DG)(DT)(DC)(DA)(DG)(DC)(DC) DNA DNA (43-MER) synthetic construct 0.015182787 1 (DG)(DG)(DC)(DT)(DG)(DA)(DC)(DG)(DC)(DC)(DT)(DC)(DG)(DA)(DG)(DA)(DA)(DA)(DC)(DA) (DC)(DC)(DA)(DG)(DA)(DA)(DC)(DG)(DA)(DG)(DT)(DA)(DG)(DT)(DA)(DA)(DA)(DT)(DT)(DG) (DG)(DA)(DA)(DG)(DA)(DC)(DA)(DT)(DC) DNA CRISPR-associated endonuclease Cas1 Streptococcus thermophilus DGCC 7710 0.034854875
C-terminal Histag
2 Escherichia coli BL21(DE3) LEVO MAGWRTVVVNIHSKLSYKNNHLIFRNSYKTEMIHLSEIDILLLETTDIVLTTMLVKRLVDENILVIFCDDKRLPTAFLTP YYARHDSSLQIARQIAWKENVKCEVWTAIIAQKILNQSYYLGECSFFEKSQSIMELYHGLERFDPSNREGHSARIYFNTL FGNDFTRESDNDINAALDYGYTLLLSMFAREVVVCGCMTQIGLKHANQFNQFNLASDIMEPFRPIIDRIVYQNRHNNFVK IKKELFSIFSETYLYNGKEMYLSNIVSDYTKKVIKALNQLGEEIPEFRMSGHHHHHH G3ECR2 3.1.-.- CRISPR-associated endoribonuclease Cas2 Streptococcus thermophilus DGCC 7710 0.013431559999999999 1 Escherichia coli BL21(DE3) LEVO MSYRYMRMILMFDMPTDTAEERKAYRKFRKFLLSEGFIMHQFSVYSKLLLNHTANTAMVGRLKANNPKKGNITILTVTEK QFARMIYLYGDKNTSIANSEERLVFLGDNYCDED G3ECR3 3.1.-.- CRISPR-associated protein Csn2 Streptococcus thermophilus DGCC 7710 0.027459561 4 Escherichia coli BL21(DE3) LEVO MGHHHHHHENLYFQGGMKINFSLLDEPMEVNLGTVLVIEDVSVFAQLVKEFYQYDEQSNLTIFDSKIRSIRSSELLLITD ILGYDINTSQVLKLLHTDIVSQLNDKPEVRSEIDSLVSLITDIIMAECIENELDIEYDEITLLELIKALGVRIETKSCTV FEKIFEILQIFKYLVKKRILVFVNSLSYFSKDEIYQILEYTKLSQADVLFLEPRQIEGIQQFILDKDYILMPYNN G3ECR4 CALCIUM ION 4.0078e-05 1 CA singleParticle particle 7.5 Quantifoil R1.2/1.3 COPPER 300 GLOW DISCHARGE AIR ETHANE FEI VITROBOT MARK IV TFS GLACIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 200 50.0 2.7 1.0 2.0 92000.0 NITROGEN FEI FALCON III (4k x 4k) COUNTING 46.33 30.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION cryoSPARC ab-initio C1 FOURIER SPACE 3.62 FSC 0.143 CUT-OFF cryoSPARC 4.6.2 24394 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_52886.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 336 336 336 0 0 0 336 336 336 369.6 369.6 369.6 90.0 90.0 90.0 Z Y X -7.6418295 29.663097 0.001021816 1.0077693 1.1 1.1 1.1 6.0 AUTHOR Unsharpened composite map 9h2g PDB experimental model emd_52886_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 336 336 336 0 0 0 336 336 336 369.6 369.6 369.6 90.0 90.0 90.0 Z Y X -27.117273000000001 35.50705 0.000000000001686 1.0 1.1 1.1 1.1 AUTHOR Sharpened composite map. Obtained with phenix.auto_sharpen_1.21.2-5419 b_iso_to_d procedure, 3.6A