data_EMD-52886
# 
_entry.id   EMD-52886 
# 
_audit_conform.dict_name       mmcif_pdbx.dic 
_audit_conform.dict_version    5.410 
_audit_conform.dict_location   http://mmcif.pdb.org/dictionaries/ascii/mmcif_pdbx.dic 
# 
loop_
_database_2.database_id 
_database_2.database_code 
_database_2.pdbx_database_accession 
_database_2.pdbx_DOI 
PDB   9Q85         pdb_00009q85 10.2210/pdb9q85/pdb 
WWPDB D_1292145710 ?            ?                   
EMDB  EMD-52886    ?            ?                   
# 
loop_
_pdbx_audit_revision_history.ordinal 
_pdbx_audit_revision_history.data_content_type 
_pdbx_audit_revision_history.major_revision 
_pdbx_audit_revision_history.minor_revision 
_pdbx_audit_revision_history.revision_date 
_pdbx_audit_revision_history.part_number 
1 'Structure model' 1 0 2026-02-18 ? 
2 'EM metadata'     1 0 2026-02-18 ? 
3 'Additional map'  1 0 2026-02-18 1 
4 Image             1 0 2026-02-18 ? 
5 'Primary map'     1 0 2026-02-18 ? 
6 'Structure model' 1 1 2026-03-04 ? 
7 'EM metadata'     1 1 2026-03-04 ? 
8 'Structure model' 1 2 2026-03-18 ? 
9 'EM metadata'     1 2 2026-03-18 ? 
# 
loop_
_pdbx_audit_revision_details.ordinal 
_pdbx_audit_revision_details.revision_ordinal 
_pdbx_audit_revision_details.data_content_type 
_pdbx_audit_revision_details.provider 
_pdbx_audit_revision_details.type 
_pdbx_audit_revision_details.description 
_pdbx_audit_revision_details.details 
1 1 'Structure model' repository 'Initial release' ? ? 
2 2 'EM metadata'     repository 'Initial release' ? ? 
3 3 'Additional map'  repository 'Initial release' ? ? 
4 4 Image             repository 'Initial release' ? ? 
5 5 'Primary map'     repository 'Initial release' ? ? 
# 
loop_
_pdbx_audit_revision_group.ordinal 
_pdbx_audit_revision_group.revision_ordinal 
_pdbx_audit_revision_group.data_content_type 
_pdbx_audit_revision_group.group 
1 6 'Structure model' 'Data collection'      
2 6 'Structure model' 'Database references'  
3 7 'EM metadata'     'Database references'  
4 7 'EM metadata'     'Experimental summary' 
5 8 'Structure model' 'Data collection'      
6 8 'Structure model' 'Database references'  
7 9 'EM metadata'     'Database references'  
8 9 'EM metadata'     'Experimental summary' 
# 
loop_
_pdbx_audit_revision_category.ordinal 
_pdbx_audit_revision_category.revision_ordinal 
_pdbx_audit_revision_category.data_content_type 
_pdbx_audit_revision_category.category 
1  6 'Structure model' citation        
2  6 'Structure model' citation_author 
3  6 'Structure model' em_admin        
4  7 'EM metadata'     citation        
5  7 'EM metadata'     citation_author 
6  7 'EM metadata'     em_admin        
7  8 'Structure model' citation        
8  8 'Structure model' em_admin        
9  9 'EM metadata'     citation        
10 9 'EM metadata'     em_admin        
# 
loop_
_pdbx_audit_revision_item.ordinal 
_pdbx_audit_revision_item.revision_ordinal 
_pdbx_audit_revision_item.data_content_type 
_pdbx_audit_revision_item.item 
1  6 'Structure model' '_citation.country'                 
2  6 'Structure model' '_citation.journal_abbrev'          
3  6 'Structure model' '_citation.journal_id_ASTM'         
4  6 'Structure model' '_citation.journal_id_CSD'          
5  6 'Structure model' '_citation.journal_id_ISSN'         
6  6 'Structure model' '_citation.pdbx_database_id_DOI'    
7  6 'Structure model' '_citation.pdbx_database_id_PubMed' 
8  6 'Structure model' '_citation.title'                   
9  6 'Structure model' '_citation.year'                    
10 6 'Structure model' '_em_admin.last_update'             
11 7 'EM metadata'     '_citation.country'                 
12 7 'EM metadata'     '_citation.journal_abbrev'          
13 7 'EM metadata'     '_citation.journal_id_ASTM'         
14 7 'EM metadata'     '_citation.journal_id_CSD'          
15 7 'EM metadata'     '_citation.journal_id_ISSN'         
16 7 'EM metadata'     '_citation.pdbx_database_id_DOI'    
17 7 'EM metadata'     '_citation.pdbx_database_id_PubMed' 
18 7 'EM metadata'     '_citation.title'                   
19 7 'EM metadata'     '_citation.year'                    
20 7 'EM metadata'     '_em_admin.last_update'             
21 8 'Structure model' '_citation.journal_volume'          
22 8 'Structure model' '_citation.page_first'              
23 8 'Structure model' '_citation.page_last'               
24 8 'Structure model' '_em_admin.last_update'             
25 9 'EM metadata'     '_citation.journal_volume'          
26 9 'EM metadata'     '_citation.page_first'              
27 9 'EM metadata'     '_citation.page_last'               
28 9 'EM metadata'     '_em_admin.last_update'             
# 
loop_
_pdbx_database_related.db_name 
_pdbx_database_related.details 
_pdbx_database_related.db_id 
_pdbx_database_related.content_type 
EMDB 'Consensus map'                                                                                                              
EMD-52883 'consensus EM volume'  
EMDB 'Focused map 1, mask covers Cas9 and Cas1 subunits'                                                                          
EMD-52884 'focused EM volume'    
EMDB 'Focused map 2, mask covers Cas1 and Csn2 subunits'                                                                          
EMD-52885 'focused EM volume'    
EMDB 'PAM-bound Cas9-Cas1-Cas2-Csn2 supercomplex in the locked conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system' 
EMD-52886 'associated EM volume' 
# 
_citation.abstract                  ? 
_citation.abstract_id_CAS           ? 
_citation.book_id_ISBN              ? 
_citation.book_publisher            ? 
_citation.book_publisher_city       ? 
_citation.book_title                ? 
_citation.coordinate_linkage        ? 
_citation.country                   US 
_citation.database_id_Medline       ? 
_citation.details                   ? 
_citation.id                        primary 
_citation.journal_abbrev            Mol.Cell 
_citation.journal_id_ASTM           MOCEFL 
_citation.journal_id_CSD            2168 
_citation.journal_id_ISSN           1097-2765 
_citation.journal_full              ? 
_citation.journal_issue             ? 
_citation.journal_volume            86 
_citation.language                  ? 
_citation.page_first                791 
_citation.page_last                 804.e9 
_citation.title                     'Structural insights into Cas9-mediated prespacer selection in CRISPR-Cas adaptation.' 
_citation.year                      2026 
_citation.database_id_CSD           ? 
_citation.pdbx_database_id_DOI      10.1016/j.molcel.2026.01.022 
_citation.pdbx_database_id_PubMed   41702403 
_citation.pdbx_database_id_patent   ? 
_citation.unpublished_flag          ? 
# 
loop_
_citation_author.citation_id 
_citation_author.name 
_citation_author.ordinal 
_citation_author.identifier_ORCID 
primary 'Gaizauskaite, U.' 1 ? 
primary 'Tamulaitiene, G.' 2 ? 
primary 'Silanskas, A.'    3 ? 
primary 'Gasiunas, G.'     4 ? 
primary 'Siksnys, V.'      5 ? 
primary 'Sasnauskas, G.'   6 ? 
# 
loop_
_entity.id 
_entity.type 
_entity.src_method 
_entity.pdbx_description 
_entity.formula_weight 
_entity.pdbx_number_of_molecules 
_entity.pdbx_ec 
_entity.pdbx_mutation 
_entity.pdbx_fragment 
_entity.details 
1 polymer     man 'CRISPR-associated endonuclease Cas9'     162498.531 1 3.1.-.- ? ? ?                   
2 polymer     syn crRNA                                     13609.083  1 ?       ? ? ?                   
3 polymer     syn tracrRNA                                  24119.357  1 ?       ? ? ?                   
4 polymer     syn 'DNA (43-MER)'                            15003.586  1 ?       ? ? ?                   
5 polymer     syn 'DNA (43-MER)'                            15182.787  1 ?       ? ? ?                   
6 polymer     man 'CRISPR-associated endonuclease Cas1'     34854.875  2 3.1.-.- ? ? 'C-terminal Histag' 
7 polymer     man 'CRISPR-associated endoribonuclease Cas2' 13431.560  1 3.1.-.- ? ? ?                   
8 polymer     man 'CRISPR-associated protein Csn2'          27459.561  4 ?       ? ? ?                   
9 non-polymer syn 'CALCIUM ION'                             40.078     1 ?       ? ? ?                   
# 
loop_
_entity_poly.entity_id 
_entity_poly.type 
_entity_poly.pdbx_seq_one_letter_code 
1 'polypeptide(L)'        
;MTKPYSIGLDIGTNSVGWAVITDNYKVPSKKMKVLGNTSKKYIKKNLLGVLLFDSGITAEGRRLKRTARRRYTRRRNRIL

LLKDATLIHQSVTGLYETRIDLAKLGEGLEGHHHHHH
;
2 polyribonucleotide      CGCUAAAGAGGAAGAGGACAGUUUUAGAGCUGUGUUGUUUCG 
3 polyribonucleotide      CGAAACAACACAGCGAGUUAAAAUAAGGCUUAGUCCGUACUCAACUUGAAAAGGUGGCACCGAUUCGGUGUUUUU 
4 polydeoxyribonucleotide 
;(DG)(DA)(DT)(DG)(DT)(DC)(DT)(DT)(DC)(DC)(DA)(DA)(DT)(DT)(DT)(DA)(DC)(DT)(DA)(DC)
(DT)(DC)(DG)(DT)(DT)(DC)(DT)(DG)(DG)(DT)(DG)(DT)(DT)(DT)(DC)(DT)(DC)(DG)(DA)(DG)
(DG)(DC)(DG)(DT)(DC)(DA)(DG)(DC)(DC)
;
5 polydeoxyribonucleotide 
;(DG)(DG)(DC)(DT)(DG)(DA)(DC)(DG)(DC)(DC)(DT)(DC)(DG)(DA)(DG)(DA)(DA)(DA)(DC)(DA)
(DC)(DC)(DA)(DG)(DA)(DA)(DC)(DG)(DA)(DG)(DT)(DA)(DG)(DT)(DA)(DA)(DA)(DT)(DT)(DG)
(DG)(DA)(DA)(DG)(DA)(DC)(DA)(DT)(DC)
;
6 'polypeptide(L)'        
;MAGWRTVVVNIHSKLSYKNNHLIFRNSYKTEMIHLSEIDILLLETTDIVLTTMLVKRLVDENILVIFCDDKRLPTAFLTP
YYARHDSSLQIARQIAWKENVKCEVWTAIIAQKILNQSYYLGECSFFEKSQSIMELYHGLERFDPSNREGHSARIYFNTL
FGNDFTRESDNDINAALDYGYTLLLSMFAREVVVCGCMTQIGLKHANQFNQFNLASDIMEPFRPIIDRIVYQNRHNNFVK
IKKELFSIFSETYLYNGKEMYLSNIVSDYTKKVIKALNQLGEEIPEFRMSGHHHHHH
;
7 'polypeptide(L)'        
;MSYRYMRMILMFDMPTDTAEERKAYRKFRKFLLSEGFIMHQFSVYSKLLLNHTANTAMVGRLKANNPKKGNITILTVTEK
QFARMIYLYGDKNTSIANSEERLVFLGDNYCDED
;
8 'polypeptide(L)'        
;MGHHHHHHENLYFQGGMKINFSLLDEPMEVNLGTVLVIEDVSVFAQLVKEFYQYDEQSNLTIFDSKIRSIRSSELLLITD
ILGYDINTSQVLKLLHTDIVSQLNDKPEVRSEIDSLVSLITDIIMAECIENELDIEYDEITLLELIKALGVRIETKSCTV
FEKIFEILQIFKYLVKKRILVFVNSLSYFSKDEIYQILEYTKLSQADVLFLEPRQIEGIQQFILDKDYILMPYNN
;
# 
_pdbx_entity_nonpoly.entity_id   9 
_pdbx_entity_nonpoly.name        'CALCIUM ION' 
_pdbx_entity_nonpoly.comp_id     CA 
# 
loop_
_entity_src_gen.entity_id 
_entity_src_gen.pdbx_src_id 
_entity_src_gen.pdbx_alt_source_flag 
_entity_src_gen.pdbx_seq_type 
_entity_src_gen.pdbx_beg_seq_num 
_entity_src_gen.pdbx_end_seq_num 
_entity_src_gen.gene_src_common_name 
_entity_src_gen.gene_src_genus 
_entity_src_gen.pdbx_gene_src_gene 
_entity_src_gen.gene_src_species 
_entity_src_gen.gene_src_strain 
_entity_src_gen.gene_src_tissue 
_entity_src_gen.gene_src_tissue_fraction 
_entity_src_gen.gene_src_details 
_entity_src_gen.pdbx_gene_src_fragment 
_entity_src_gen.pdbx_gene_src_scientific_name 
_entity_src_gen.pdbx_gene_src_ncbi_taxonomy_id 
_entity_src_gen.pdbx_gene_src_variant 
_entity_src_gen.pdbx_gene_src_cell_line 
_entity_src_gen.pdbx_gene_src_atcc 
_entity_src_gen.pdbx_gene_src_culture_collection 
_entity_src_gen.pdbx_gene_src_organ 
_entity_src_gen.pdbx_gene_src_organelle 
_entity_src_gen.pdbx_gene_src_cell 
_entity_src_gen.pdbx_gene_src_cellular_location 
_entity_src_gen.host_org_common_name 
_entity_src_gen.pdbx_host_org_scientific_name 
_entity_src_gen.pdbx_host_org_ncbi_taxonomy_id 
_entity_src_gen.host_org_genus 
_entity_src_gen.pdbx_host_org_gene 
_entity_src_gen.pdbx_host_org_organ 
_entity_src_gen.host_org_species 
_entity_src_gen.pdbx_host_org_tissue 
_entity_src_gen.pdbx_host_org_tissue_fraction 
_entity_src_gen.pdbx_host_org_strain 
_entity_src_gen.pdbx_host_org_variant 
_entity_src_gen.pdbx_host_org_cell_line 
_entity_src_gen.pdbx_host_org_atcc 
_entity_src_gen.pdbx_host_org_culture_collection 
_entity_src_gen.pdbx_host_org_cell 
_entity_src_gen.pdbx_host_org_organelle 
_entity_src_gen.pdbx_host_org_cellular_location 
_entity_src_gen.pdbx_host_org_vector_type 
_entity_src_gen.pdbx_host_org_vector 
_entity_src_gen.host_org_details 
_entity_src_gen.expression_system_id 
_entity_src_gen.plasmid_name 
_entity_src_gen.plasmid_details 
_entity_src_gen.pdbx_description 
1 1 sample 'Biological sequence' 1 1397 ? ? 'cas9, csn1' ? ? ? ? ? ? 'Streptococcus thermophilus DGCC 7710' 1268061 ? ? ? ? ? ? ? 
? ? 'Escherichia coli BL21(DE3)' 469008 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
6 1 sample 'Biological sequence' 1 297  ? ? cas1         ? ? ? ? ? ? 'Streptococcus thermophilus DGCC 7710' 1268061 ? ? ? ? ? ? ? 
? ? 'Escherichia coli BL21(DE3)' 469008 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
7 1 sample 'Biological sequence' 1 114  ? ? cas2         ? ? ? ? ? ? 'Streptococcus thermophilus DGCC 7710' 1268061 ? ? ? ? ? ? ? 
? ? 'Escherichia coli BL21(DE3)' 469008 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
8 1 sample 'Biological sequence' 1 235  ? ? csn2         ? ? ? ? ? ? 'Streptococcus thermophilus DGCC 7710' 1268061 ? ? ? ? ? ? ? 
? ? 'Escherichia coli BL21(DE3)' 469008 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 
# 
loop_
_pdbx_entity_src_syn.entity_id 
_pdbx_entity_src_syn.pdbx_src_id 
_pdbx_entity_src_syn.pdbx_alt_source_flag 
_pdbx_entity_src_syn.pdbx_beg_seq_num 
_pdbx_entity_src_syn.pdbx_end_seq_num 
_pdbx_entity_src_syn.organism_scientific 
_pdbx_entity_src_syn.organism_common_name 
_pdbx_entity_src_syn.ncbi_taxonomy_id 
_pdbx_entity_src_syn.details 
2 1 sample 1 42 'synthetic construct' ? 32630 ? 
3 1 sample 1 75 'synthetic construct' ? 32630 ? 
4 1 sample 1 49 'synthetic construct' ? 32630 ? 
5 1 sample 1 49 'synthetic construct' ? 32630 ? 
# 
loop_
_chem_comp.id 
_chem_comp.type 
_chem_comp.mon_nstd_flag 
_chem_comp.name 
_chem_comp.pdbx_synonyms 
_chem_comp.formula 
_chem_comp.formula_weight 
A   'RNA linking'       y "ADENOSINE-5'-MONOPHOSPHATE"         ? 'C10 H14 N5 O7 P' 347.221 
ALA 'L-peptide linking' y ALANINE                              ? 'C3 H7 N O2'      89.093  
ARG 'L-peptide linking' y ARGININE                             ? 'C6 H15 N4 O2 1'  175.209 
ASN 'L-peptide linking' y ASPARAGINE                           ? 'C4 H8 N2 O3'     132.118 
ASP 'L-peptide linking' y 'ASPARTIC ACID'                      ? 'C4 H7 N O4'      133.103 
C   'RNA linking'       y "CYTIDINE-5'-MONOPHOSPHATE"          ? 'C9 H14 N3 O8 P'  323.197 
CA  non-polymer         . 'CALCIUM ION'                        ? 'Ca 2'            40.078  
CYS 'L-peptide linking' y CYSTEINE                             ? 'C3 H7 N O2 S'    121.158 
DA  'DNA linking'       y "2'-DEOXYADENOSINE-5'-MONOPHOSPHATE" ? 'C10 H14 N5 O6 P' 331.222 
DC  'DNA linking'       y "2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE"  ? 'C9 H14 N3 O7 P'  307.197 
DG  'DNA linking'       y "2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE" ? 'C10 H14 N5 O7 P' 347.221 
DT  'DNA linking'       y "THYMIDINE-5'-MONOPHOSPHATE"         ? 'C10 H15 N2 O8 P' 322.208 
G   'RNA linking'       y "GUANOSINE-5'-MONOPHOSPHATE"         ? 'C10 H14 N5 O8 P' 363.221 
GLN 'L-peptide linking' y GLUTAMINE                            ? 'C5 H10 N2 O3'    146.144 
GLU 'L-peptide linking' y 'GLUTAMIC ACID'                      ? 'C5 H9 N O4'      147.129 
GLY 'peptide linking'   y GLYCINE                              ? 'C2 H5 N O2'      75.067  
HIS 'L-peptide linking' y HISTIDINE                            ? 'C6 H10 N3 O2 1'  156.162 
ILE 'L-peptide linking' y ISOLEUCINE                           ? 'C6 H13 N O2'     131.173 
LEU 'L-peptide linking' y LEUCINE                              ? 'C6 H13 N O2'     131.173 
LYS 'L-peptide linking' y LYSINE                               ? 'C6 H15 N2 O2 1'  147.195 
MET 'L-peptide linking' y METHIONINE                           ? 'C5 H11 N O2 S'   149.211 
PHE 'L-peptide linking' y PHENYLALANINE                        ? 'C9 H11 N O2'     165.189 
PRO 'L-peptide linking' y PROLINE                              ? 'C5 H9 N O2'      115.130 
SER 'L-peptide linking' y SERINE                               ? 'C3 H7 N O3'      105.093 
THR 'L-peptide linking' y THREONINE                            ? 'C4 H9 N O3'      119.119 
TRP 'L-peptide linking' y TRYPTOPHAN                           ? 'C11 H12 N2 O2'   204.225 
TYR 'L-peptide linking' y TYROSINE                             ? 'C9 H11 N O3'     181.189 
U   'RNA linking'       y "URIDINE-5'-MONOPHOSPHATE"           ? 'C9 H13 N2 O9 P'  324.181 
VAL 'L-peptide linking' y VALINE                               ? 'C5 H11 N O2'     117.146 
# 
_exptl.entry_id         EMD-52886 
_exptl.details          ? 
_exptl.method           'ELECTRON MICROSCOPY' 
_exptl.method_details   ? 
# 
_struct_keywords.entry_id        EMD-52886 
_struct_keywords.text            'CIRSPR-Cas, spacer acquisition, Csn2, Cas1, Cas2, CRISPR integrase, DNA BINDING PROTEIN' 
_struct_keywords.pdbx_keywords   'DNA BINDING PROTEIN' 
_struct_keywords.pdbx_details    ? 
# 
loop_
_struct_ref.id 
_struct_ref.db_name 
_struct_ref.db_code 
_struct_ref.entity_id 
1 UNP CAS9_STRTR 1 
2 PDB 9Q85       2 
3 PDB 9Q85       3 
4 PDB 9Q85       4 
5 PDB 9Q85       5 
6 UNP CAS1_STRTR 6 
7 UNP CAS2_STRTR 7 
8 UNP CSN2_STRTR 8 
# 
_em_3d_fitting.id                1 
_em_3d_fitting.entry_id          EMD-52886 
_em_3d_fitting.method            ? 
_em_3d_fitting.target_criteria   ? 
_em_3d_fitting.details           ? 
_em_3d_fitting.overall_b_value   ? 
_em_3d_fitting.ref_space         ? 
_em_3d_fitting.ref_protocol      ? 
# 
_em_3d_fitting_list.id                            1 
_em_3d_fitting_list.3d_fitting_id                 1 
_em_3d_fitting_list.pdb_entry_id                  9h2g 
_em_3d_fitting_list.pdb_chain_id                  ? 
_em_3d_fitting_list.pdb_chain_residue_range       ? 
_em_3d_fitting_list.details                       ? 
_em_3d_fitting_list.chain_id                      ? 
_em_3d_fitting_list.chain_residue_range           ? 
_em_3d_fitting_list.source_name                   PDB 
_em_3d_fitting_list.type                          'experimental model' 
_em_3d_fitting_list.accession_code                9h2g 
_em_3d_fitting_list.initial_refinement_model_id   1 
# 
_em_3d_reconstruction.entry_id                    EMD-52886 
_em_3d_reconstruction.id                          1 
_em_3d_reconstruction.method                      ? 
_em_3d_reconstruction.algorithm                   'FOURIER SPACE' 
_em_3d_reconstruction.citation_id                 ? 
_em_3d_reconstruction.details                     ? 
_em_3d_reconstruction.resolution                  3.62 
_em_3d_reconstruction.resolution_method           'FSC 0.143 CUT-OFF' 
_em_3d_reconstruction.magnification_calibration   ? 
_em_3d_reconstruction.nominal_pixel_size          ? 
_em_3d_reconstruction.actual_pixel_size           ? 
_em_3d_reconstruction.num_particles               24394 
_em_3d_reconstruction.euler_angles_details        ? 
_em_3d_reconstruction.num_class_averages          ? 
_em_3d_reconstruction.fsc_type                    'EVEN/ODD MAPS REFINED TOTALLY INDEPENDENT (GOLD STANDARD)' 
_em_3d_reconstruction.refinement_type             ? 
_em_3d_reconstruction.image_processing_id         1 
_em_3d_reconstruction.symmetry_type               POINT 
# 
_em_buffer.id            1 
_em_buffer.specimen_id   1 
_em_buffer.name          ? 
_em_buffer.details       ? 
_em_buffer.pH            7.5 
# 
_em_entity_assembly.id                   1 
_em_entity_assembly.parent_id            0 
_em_entity_assembly.source               RECOMBINANT 
_em_entity_assembly.type                 COMPLEX 
_em_entity_assembly.name                 
'PAM-bound Cas9-Cas1-Cas2-Csn2 supercomplex in the locked conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system' 
_em_entity_assembly.details              ? 
_em_entity_assembly.go_id                ? 
_em_entity_assembly.ipr_id               ? 
_em_entity_assembly.synonym              ? 
_em_entity_assembly.number_of_copies     ? 
_em_entity_assembly.oligomeric_details   ? 
_em_entity_assembly.entity_id_list       '1, 2, 3, 4, 5, 6, 7, 8' 
_em_entity_assembly.mutant_flag          ? 
_em_entity_assembly.chimera              NO 
# 
_em_imaging.entry_id                        EMD-52886 
_em_imaging.id                              1 
_em_imaging.astigmatism                     ? 
_em_imaging.electron_beam_tilt_params       ? 
_em_imaging.residual_tilt                   ? 
_em_imaging.sample_support_id               1 
_em_imaging.detector_id                     1 
_em_imaging.scans_id                        1 
_em_imaging.microscope_id                   ? 
_em_imaging.microscope_model                'TFS GLACIOS' 
_em_imaging.specimen_holder_type            ? 
_em_imaging.specimen_holder_model           ? 
_em_imaging.details                         ? 
_em_imaging.date                            ? 
_em_imaging.accelerating_voltage            200 
_em_imaging.illumination_mode               OTHER 
_em_imaging.mode                            'BRIGHT FIELD' 
_em_imaging.nominal_cs                      2.7 
_em_imaging.nominal_defocus_min             1000 
_em_imaging.nominal_defocus_max             2000 
_em_imaging.calibrated_defocus_min          ? 
_em_imaging.calibrated_defocus_max          ? 
_em_imaging.tilt_angle_min                  ? 
_em_imaging.tilt_angle_max                  ? 
_em_imaging.nominal_magnification           92000 
_em_imaging.calibrated_magnification        ? 
_em_imaging.electron_source                 'FIELD EMISSION GUN' 
_em_imaging.citation_id                     ? 
_em_imaging.temperature                     ? 
_em_imaging.detector_distance               ? 
_em_imaging.recording_temperature_minimum   ? 
_em_imaging.recording_temperature_maximum   ? 
_em_imaging.alignment_procedure             ? 
_em_imaging.c2_aperture_diameter            50 
_em_imaging.specimen_id                     1 
_em_imaging.cryogen                         NITROGEN 
_em_imaging.objective_aperture              ? 
_em_imaging.microscope_serial_number        ? 
_em_imaging.microscope_version              ? 
# 
_em_sample_support.id               1 
_em_sample_support.film_material    ? 
_em_sample_support.method           ? 
_em_sample_support.grid_material    COPPER 
_em_sample_support.grid_mesh_size   300 
_em_sample_support.grid_type        'Quantifoil R1.2/1.3' 
_em_sample_support.pretreatment     ? 
_em_sample_support.details          ? 
_em_sample_support.specimen_id      1 
_em_sample_support.citation_id      ? 
# 
_em_vitrification.entry_id                EMD-52886 
_em_vitrification.id                      1 
_em_vitrification.sample_preparation_id   ? 
_em_vitrification.specimen_id             1 
_em_vitrification.cryogen_name            ETHANE 
_em_vitrification.humidity                ? 
_em_vitrification.temp                    ? 
_em_vitrification.chamber_temperature     ? 
_em_vitrification.instrument              'FEI VITROBOT MARK IV' 
_em_vitrification.method                  ? 
_em_vitrification.time_resolved_state     ? 
_em_vitrification.citation_id             ? 
_em_vitrification.details                 ? 
# 
_em_experiment.entry_id                EMD-52886 
_em_experiment.id                      1 
_em_experiment.reconstruction_method   'SINGLE PARTICLE' 
_em_experiment.aggregation_state       PARTICLE 
_em_experiment.entity_assembly_id      1 
# 
_em_single_particle_entity.entry_id              EMD-52886 
_em_single_particle_entity.id                    1 
_em_single_particle_entity.image_processing_id   1 
_em_single_particle_entity.point_symmetry        C1 
# 
_em_admin.current_status        REL 
_em_admin.deposition_date       2025-02-22 
_em_admin.deposition_site       PDBE 
_em_admin.details               ? 
_em_admin.entry_id              EMD-52886 
_em_admin.last_update           2026-03-18 
_em_admin.map_release_date      2026-02-18 
_em_admin.header_release_date   2026-02-18 
_em_admin.obsoleted_date        ? 
_em_admin.title                 
'PAM-bound Cas9-Cas1-Cas2-Csn2 supercomplex in the locked conformation, Streptococcus thermophilus DGCC 7710 CRISPR3 system' 
_em_admin.process_site          PDBE 
_em_admin.composite_map         YES 
# 
loop_
_em_author_list.author 
_em_author_list.identifier_ORCID 
_em_author_list.ordinal 
'Sasnauskas, G.'   ? 1 
'Gaizauskaite, U.' ? 2 
'Tamulaitiene, G.' ? 3 
# 
_em_ctf_correction.amplitude_correction              ? 
_em_ctf_correction.amplitude_correction_factor       ? 
_em_ctf_correction.amplitude_correction_space        ? 
_em_ctf_correction.correction_operation              ? 
_em_ctf_correction.details                           ? 
_em_ctf_correction.em_image_processing_id            1 
_em_ctf_correction.id                                1 
_em_ctf_correction.phase_reversal                    ? 
_em_ctf_correction.phase_reversal_anisotropic        ? 
_em_ctf_correction.phase_reversal_correction_space   ? 
_em_ctf_correction.type                              'PHASE FLIPPING AND AMPLITUDE CORRECTION' 
# 
_em_db_reference.access_code    9Q85 
_em_db_reference.db_name        PDB 
_em_db_reference.details        ? 
_em_db_reference.id             1 
_em_db_reference.relationship   'IN FRAME' 
# 
_em_entity_assembly_molwt.entity_assembly_id   1 
_em_entity_assembly_molwt.experimental_flag    NO 
_em_entity_assembly_molwt.id                   1 
_em_entity_assembly_molwt.units                ? 
_em_entity_assembly_molwt.value                ? 
_em_entity_assembly_molwt.method               ? 
# 
_em_entity_assembly_naturalsource.cell                 ? 
_em_entity_assembly_naturalsource.cellular_location    ? 
_em_entity_assembly_naturalsource.entity_assembly_id   1 
_em_entity_assembly_naturalsource.id                   2 
_em_entity_assembly_naturalsource.ncbi_tax_id          1268061 
_em_entity_assembly_naturalsource.organism             'Streptococcus thermophilus DGCC 7710' 
_em_entity_assembly_naturalsource.organelle            ? 
_em_entity_assembly_naturalsource.organ                ? 
_em_entity_assembly_naturalsource.strain               ? 
_em_entity_assembly_naturalsource.tissue               ? 
_em_entity_assembly_naturalsource.details              ? 
# 
_em_entity_assembly_recombinant.cell                 ? 
_em_entity_assembly_recombinant.entity_assembly_id   1 
_em_entity_assembly_recombinant.id                   2 
_em_entity_assembly_recombinant.ncbi_tax_id          562 
_em_entity_assembly_recombinant.organism             'Escherichia coli' 
_em_entity_assembly_recombinant.plasmid              ? 
_em_entity_assembly_recombinant.strain               ? 
# 
loop_
_em_euler_angle_assignment.details 
_em_euler_angle_assignment.id 
_em_euler_angle_assignment.image_processing_id 
_em_euler_angle_assignment.order 
_em_euler_angle_assignment.proj_matching_angular_sampling 
_em_euler_angle_assignment.proj_matching_merit_function 
_em_euler_angle_assignment.proj_matching_num_projections 
_em_euler_angle_assignment.type 
? 1 1 INITIAL ? ? ? 'MAXIMUM LIKELIHOOD' 
? 2 1 FINAL   ? ? ? 'MAXIMUM LIKELIHOOD' 
# 
_em_grid_pretreatment.atmosphere          air 
_em_grid_pretreatment.id                  1 
_em_grid_pretreatment.pressure            ? 
_em_grid_pretreatment.sample_support_id   1 
_em_grid_pretreatment.time                20 
_em_grid_pretreatment.type                'GLOW DISCHARGE' 
# 
_em_image_processing.details              ? 
_em_image_processing.id                   1 
_em_image_processing.image_recording_id   1 
# 
_em_image_recording.average_exposure_time               46.33 
_em_image_recording.avg_electron_dose_per_subtomogram   ? 
_em_image_recording.avg_electron_dose_per_image         30 
_em_image_recording.details                             ? 
_em_image_recording.detector_mode                       COUNTING 
_em_image_recording.film_or_detector_model              'FEI FALCON III (4k x 4k)' 
_em_image_recording.id                                  1 
_em_image_recording.imaging_id                          1 
_em_image_recording.num_diffraction_images              ? 
_em_image_recording.num_grids_imaged                    ? 
_em_image_recording.num_real_images                     ? 
# 
loop_
_em_map.annotation_details 
_em_map.axis_order_fast 
_em_map.axis_order_medium 
_em_map.axis_order_slow 
_em_map.cell_a 
_em_map.cell_b 
_em_map.cell_c 
_em_map.cell_alpha 
_em_map.cell_beta 
_em_map.cell_gamma 
_em_map.contour_level 
_em_map.contour_level_source 
_em_map.data_type 
_em_map.dimensions_col 
_em_map.dimensions_row 
_em_map.dimensions_sec 
_em_map.endian_type 
_em_map.file 
_em_map.format 
_em_map.id 
_em_map.partition 
_em_map.entry_id 
_em_map.label 
_em_map.limit_col 
_em_map.limit_row 
_em_map.limit_sec 
_em_map.origin_col 
_em_map.origin_row 
_em_map.origin_sec 
_em_map.pixel_spacing_x 
_em_map.pixel_spacing_y 
_em_map.pixel_spacing_z 
_em_map.size_kb 
_em_map.spacing_x 
_em_map.spacing_y 
_em_map.spacing_z 
_em_map.statistics_average 
_em_map.statistics_maximum 
_em_map.statistics_minimum 
_em_map.statistics_std 
_em_map.symmetry_space_group 
_em_map.type 
'Unsharpened composite map'                                                                         Z Y X 369.6 369.6 369.6 90.0 
90.0 90.0 6 author 'Image stored as floating point number (4 bytes)' 336 336 336 big emd_52886.map.gz              CCP4 1 1 
EMD-52886 ::::EMDATABANK.org::::EMD-52886:::: 335 335 335 0 0 0 1.1 1.1 1.1 151733248 336 336 336 0.001021816   29.663097 
-7.6418295 1.0077693 1 'primary map'    
'Sharpened composite map. Obtained with phenix.auto_sharpen_1.21.2-5419 b_iso_to_d procedure, 3.6A' Z Y X 369.6 369.6 369.6 90.0 
90.0 90.0 5 author 'Image stored as floating point number (4 bytes)' 336 336 336 big emd_52886_additional_1.map.gz CCP4 2 1 
EMD-52886 ::::EMDATABANK.org::::EMD-52886:::: 335 335 335 0 0 0 1.1 1.1 1.1 151733248 336 336 336 1.6859196E-12 35.50705  
-27.117273 1.0       1 'additional map' 
# 
loop_
_em_software.category 
_em_software.details 
_em_software.id 
_em_software.image_processing_id 
_em_software.fitting_id 
_em_software.imaging_id 
_em_software.name 
_em_software.version 
'PARTICLE SELECTION'       ? 1  1 ? ? ?         ?           
'MODEL REFINEMENT'         ? 2  ? ? ? PHENIX    1.21.2_5419 
'IMAGE ACQUISITION'        ? 3  1 1 1 ?         ?           
MASKING                    ? 4  1 1 1 ?         ?           
'CTF CORRECTION'           ? 5  1 ? ? ?         ?           
'LAYERLINE INDEXING'       ? 6  1 1 1 ?         ?           
'DIFFRACTION INDEXING'     ? 7  1 1 1 ?         ?           
'MODEL FITTING'            ? 8  1 1 1 ?         ?           
OTHER                      ? 9  1 1 1 ?         ?           
'INITIAL EULER ASSIGNMENT' ? 10 1 ? ? ?         ?           
'FINAL EULER ASSIGNMENT'   ? 11 1 ? ? ?         ?           
CLASSIFICATION             ? 12 1 ? ? ?         ?           
RECONSTRUCTION             ? 13 1 ? ? cryoSPARC 4.6.2       
# 
_em_specimen.concentration           ? 
_em_specimen.details                 ? 
_em_specimen.embedding_applied       NO 
_em_specimen.experiment_id           1 
_em_specimen.id                      1 
_em_specimen.shadowing_applied       NO 
_em_specimen.staining_applied        NO 
_em_specimen.vitrification_applied   YES 
# 
_em_start_model.details                          ? 
_em_start_model.emdb_id                          ? 
_em_start_model.id                               1 
_em_start_model.image_processing_id              1 
_em_start_model.insilico_model                   'cryoSPARC ab-initio' 
_em_start_model.orthogonal_tilt_angle1           ? 
_em_start_model.orthogonal_tilt_angle2           ? 
_em_start_model.orthogonal_tilt_num_images       ? 
_em_start_model.other                            ? 
_em_start_model.pdb_id                           ? 
_em_start_model.random_conical_tilt_angle        ? 
_em_start_model.random_conical_tilt_num_images   ? 
_em_start_model.type                             'INSILICO MODEL' 
# 
_pdbx_audit_support.funding_organization   'Research Council of Lithuania' 
_pdbx_audit_support.country                Lithuania 
_pdbx_audit_support.grant_number           S-MIP-19-32 
_pdbx_audit_support.details                ? 
_pdbx_audit_support.ordinal                1 
# 
_pdbx_initial_refinement_model.id               1 
_pdbx_initial_refinement_model.entity_id_list   ? 
_pdbx_initial_refinement_model.type             'experimental model' 
_pdbx_initial_refinement_model.source_name      PDB 
_pdbx_initial_refinement_model.accession_code   9h2g 
_pdbx_initial_refinement_model.details          ? 
# 
