2025-10-29 REL PDBe PDBe PDBe 2025-02-24 2025-10-29 2025-10-29 2025-10-29 Medical Research Council (MRC, United Kingdom) MC_UU_00015/2 United Kingdom Flavoprotein (NUHM and NUBM)-containing state of Pichia pastoris mitochondrial complex I reconstituted into proteoliposomes Grba DN Hirst J NADH:ubiquinone oxidoreductase nanodisc membrane protein complex, MEMBRANE PROTEIN Lee CS Grba DN Wright JJ Ivanov BS Hirst J Global conformations of Pichia pastoris complex I are distinguished by the binding of a unique interdomain bridging subunit. Sci Adv US 11 eadz0693 eadz0693 2025 41032597 doi:10.1126/sciadv.adz0693 2375-2548 EMD-52878 consensus EM volume EMD-52877 consensus EM volume EMD-52876 consensus EM volume EMD-52875 consensus EM volume EMD-52890 consensus EM volume EMD-52891 consensus EM volume EMD-52892 consensus EM volume EMDB EMD-52878 consensus EM volume EMDB EMD-52877 consensus EM volume EMDB EMD-52876 consensus EM volume EMDB EMD-52875 consensus EM volume EMDB EMD-52890 consensus EM volume EMDB EMD-52891 consensus EM volume EMDB EMD-52892 consensus EM volume EMDB EMD-52893 associated EM volume PDB 9IHR unspecified PDB 9IHQ unspecified PDB 9IHP unspecified PDB 9IHO unspecified The mitochondrial complex I of Pichia pastoris reconstituted into proteoliposomes The mitochondrial complex I of Pichia pastoris reconstituted into proteoliposomes 0 Komagataella phaffii singleParticle particle 0.1 7.0 25.0 NaCl sodium chloride 20.0 MES
20 mM MES, 25 mM NaCL, pH 7 @4 degrees celsius
 Quantifoil R1.2/1.3 GOLD 300 GRAPHENE OXIDE CONTINUOUS GLOW DISCHARGE AIR 0.039
10 mA, 0.39 mBar, 15 seconds
 ETHANE 100 277.15 FEI VITROBOT MARK III
LMNG-solubilised Pichia pastoris CI reconstituted into proteoliposomes (8:1:1 ratio of DOPC:DOPE:CDL [0.5 mg total] and 20 nM final Q10).
 FEI TALOS ARCTICA FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 50.0 1.8 3.3000000000000003 73000.0 NITROGEN FEI FALCON III (4k x 4k) COUNTING 1 854 1.69 50.37 1 116618 RELION 4.0 PHASE FLIPPING AND AMPLITUDE CORRECTION EMD-52878
Taken from higher resolution dataset in the same publication
 3 C1 4.7 FSC 0.143 CUT-OFF RELION 4.0 20260 PROJECTION MATCHING 1 cryoSPARC 3.3. MAXIMUM LIKELIHOOD RELION 4.0 4 RELION 4.0
4 classes were run in RELION with a mask around the flavoprotein region and an increased regularisation factor of T = 50. No alignment was used in this classification.
 emd_52893.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 493.2 493.2 493.2 90.0 90.0 90.0 X Y Z -0.04923268 0.14315237 -0.00038922692 0.006090744 1.37 1.37 1.37 0.0255 AUTHOR Final consensus map. Globally sharpened (postprocessed) with relion. emd_52893_msk_1.map emd_52893_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 0 0 0 128 128 128 493.1968 493.1968 493.1968 90.0 90.0 90.0 X Y Z 0.0 1.0 0.030765383 0.16842547 3.8531 3.8531 3.8531 AUTHOR Mask used in final refine3D job in RElION (initially auto-generated in cryoSPARC). emd_52893_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 493.2 493.2 493.2 90.0 90.0 90.0 X Y Z -0.04237042 0.101043984 -0.000384094 0.0048792697 1.37 1.37 1.37 AUTHOR Half map 2 emd_52893_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 493.2 493.2 493.2 90.0 90.0 90.0 X Y Z -0.049790204 0.09371008 -0.00039435984 0.004884986 1.37 1.37 1.37 AUTHOR Half map 1