2026-01-21 REL PDBe PDBe PDBe 2025-02-27 2025-11-05 2025-11-05 2026-01-21 German Research Foundation (DFG) FR 1140/11-2 Germany German Research Foundation (DFG) 278002225 Germany Cryo-EM Map of the membrane arm of respiratory complex I V96P/N142M (nuoE) used for the creation of a composite map Seifermann T Wohlwend D Friedrich T Respiratory chain, Complex I, NADH ubiquinone oxidoreductase, electron transport, proton transport, MEMBRANE PROTEIN Wohlwend D Seifermann T Gnandt E Vranas M Gerhardt S Friedrich T Structural changes shifting the redox potential of the outlying cluster N1a in respiratory complex I. Structure UK 34 175 183.e3 2026 41265450 doi:10.1016/j.str.2025.10.016 0969-2126 2005 STRUE6 EMD-52895 other EM volume
Composite Map used for model building
 PDB 9Q8I unspecified
Composite Map used for model building
 EMDB EMD-52895 other EM volume
Composite Map used for model building
 EMDB EMD-52969 associated EM volume Respiratory complex I Respiratory complex I 0 1 2 3 4 5 6 7 8 9 10 11 12 13 Escherichia coli 0.542 singleParticle particle 10 6.0 50.0 NaCl sodium chloride 50.0 C6H13NO4S MES 5.0 MgCl2 magnesium chloride 10.0 C3H8O3 glycerol 0.005 C47H88O22 lauryl maltose neopentyl glycol
50 mM MES/NaOH, pH 6.0, 50 mM NaCl, 5 mM MgCl2, 10 % (v/v) glycerol, 0.005 % (w/v) LMNG
 Quantifoil R1.2/1.3 COPPER 200 CARBON HOLEY 12.0 ETHANE 90 277 FEI VITROBOT MARK IV
Blotting time for 6 s with filter paper.
Fos-choline-10 (0.2% (v/v) final concentration) was added before blotting. blotted for 6 s with filter paper and flash frozen in liquid ethane cooled by liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific) at 90% humidity and 277 K.
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.5 2.2 TFS FALCON 4i (4k x 4k) 40.0 1 PHASE FLIPPING AND AMPLITUDE CORRECTION 7Z7S 2.32 FSC 0.143 CUT-OFF cryoSPARC 4.7.0 95103 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_52969.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 457.60004 457.60004 457.60004 90.0 90.0 90.0 X Y Z -0.42577317 1.0823483 -0.0005603492 0.022577448 1.144 1.144 1.144 0.25 AUTHOR Focused Map of the membrane arm of respiratory complex I used for construction of a composite Map emd_52969_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 457.60004 457.60004 457.60004 90.0 90.0 90.0 X Y Z -0.724973 1.3094333 0.0000122759775 0.08091464 1.144 1.144 1.144 AUTHOR Half Map A of the focused Map of the membrane arm emd_52969_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 457.60004 457.60004 457.60004 90.0 90.0 90.0 X Y Z -0.6897644 1.338128 0.000003474302 0.08117799 1.144 1.144 1.144 AUTHOR Half Map B of the focused Map of the membrane arm